Forum for Science, Industry and Business

Sponsored by:     3M 
Search our Site:


ADI - Modified arginine deiminase as anti-tumor drug

Arginine deiminase (ADI) produces depletion of arginine and has been studied as a potential antitumor drug for the treatment of arginine-auxotrophic tumors such as hepato-cellular carcinoma (HCC) and malignant melanoma. These tumors that are sensitive to arginine depletion do not express a certain key enzyme in the synthesis of arginine from citrulline. Recent data showed that a pegylated form of ADI inhibits human melanomas and HCCs in vitro and in vivo. Furthermore, studies on human lymphatic leukemia cell lines confirmed ADI´s anti-angiogenic activity.

The main limitation for in vivo applications of numerous ADIs lies in their pH-dependant activity profile. ADI from Pseudomonas plecoglossicida (PpADI) for example has a pH optimum at 6.5. A shift from 6.5 to 7.5 (physiological conditions) results in an activity drop of approximately 80%. In order to shift the pH optimum, a directed-evolution protocol based on an adapted citrulline screening protocol in microtiter-plate format was developed and validated by the present invention. A proof of concept for ADI engineering resulted in an improved pH optimum and increased resistance under physiological and slightly alkaline conditions.

Further Information: PDF

PROvendis GmbH
Phone: +49 (0)208/94105 10

Dipl.-Ing. Alfred Schillert | TechnologieAllianz e.V.
Further information:

More articles from Technology Offerings:

nachricht Method and Device for Two-Dimensional Separation of Ionic Species
27.10.2016 | TechnologieAllianz e.V.

nachricht Shaft power plant
27.10.2016 | TechnologieAllianz e.V.

All articles from Technology Offerings >>>

The most recent press releases about innovation >>>

Die letzten 5 Focus-News des innovations-reports im Überblick:

Im Focus: Etching Microstructures with Lasers

Ultrafast lasers have introduced new possibilities in engraving ultrafine structures, and scientists are now also investigating how to use them to etch microstructures into thin glass. There are possible applications in analytics (lab on a chip) and especially in electronics and the consumer sector, where great interest has been shown.

This new method was born of a surprising phenomenon: irradiating glass in a particular way with an ultrafast laser has the effect of making the glass up to a...

Im Focus: Light-driven atomic rotations excite magnetic waves

Terahertz excitation of selected crystal vibrations leads to an effective magnetic field that drives coherent spin motion

Controlling functional properties by light is one of the grand goals in modern condensed matter physics and materials science. A new study now demonstrates how...

Im Focus: New 3-D wiring technique brings scalable quantum computers closer to reality

Researchers from the Institute for Quantum Computing (IQC) at the University of Waterloo led the development of a new extensible wiring technique capable of controlling superconducting quantum bits, representing a significant step towards to the realization of a scalable quantum computer.

"The quantum socket is a wiring method that uses three-dimensional wires based on spring-loaded pins to address individual qubits," said Jeremy Béjanin, a PhD...

Im Focus: Scientists develop a semiconductor nanocomposite material that moves in response to light

In a paper in Scientific Reports, a research team at Worcester Polytechnic Institute describes a novel light-activated phenomenon that could become the basis for applications as diverse as microscopic robotic grippers and more efficient solar cells.

A research team at Worcester Polytechnic Institute (WPI) has developed a revolutionary, light-activated semiconductor nanocomposite material that can be used...

Im Focus: Diamonds aren't forever: Sandia, Harvard team create first quantum computer bridge

By forcefully embedding two silicon atoms in a diamond matrix, Sandia researchers have demonstrated for the first time on a single chip all the components needed to create a quantum bridge to link quantum computers together.

"People have already built small quantum computers," says Sandia researcher Ryan Camacho. "Maybe the first useful one won't be a single giant quantum computer...

All Focus news of the innovation-report >>>



Event News

#IC2S2: When Social Science meets Computer Science - GESIS will host the IC2S2 conference 2017

14.10.2016 | Event News

Agricultural Trade Developments and Potentials in Central Asia and the South Caucasus

14.10.2016 | Event News

World Health Summit – Day Three: A Call to Action

12.10.2016 | Event News

Latest News

How nanoscience will improve our health and lives in the coming years

27.10.2016 | Materials Sciences

OU-led team discovers rare, newborn tri-star system using ALMA

27.10.2016 | Physics and Astronomy

'Neighbor maps' reveal the genome's 3-D shape

27.10.2016 | Life Sciences

More VideoLinks >>>