“Most microscopes can only study cell function in two dimensions,” said Dr. Gaddum Duemani Reddy, an M.D./Ph.D. student at BCM at Houston and Rice University and also first author of the study. “To look at different planes, you have move your preparation (of cells) or the objective lens. That takes time, and we are looking at processes that happen in milliseconds.”
To solve that problem, he said, they developed a “trick” to quickly move a laser beam in three dimensions and then adapted that laser beam to the multi-photon microscope they were using. That allowed them to “see” the neuron’s function in three dimensions, giving them a much better view of its activity.
A multiphoton microscope looks much like a conventional, upright microscope but it has an adaption that allows it to look at tissues in sections. A conventional multiphoton microscope does that very slowly, he said.
“With ours, you can do it very quickly. We are starting to see how a single neuron behaves in our laboratory,” he said. The next step, he said, will be to use to it to look a clusters or colonies of neurons. This will enable them to actually see the neuronal interactions.
“At present, the technology is applied in my lab to study information processing of single neurons in brain slice preparations by 3D multi-site optical recording,” said Dr. Peter Saggau, professor of neuroscience at BCM and the paper’s senior author.
He is collaborating with two other labs on using the technology in other ways. In one, he said, researchers plan to use the technology to monitor nerve activity in the brains of lab animals in order study how populations of neurons communicate during visual stimulation. Another study attempts to use the technology to monitor stimulation of the acoustic nerve optically. Those scientists hope to reinstate hearing in lab animals whose inner ear receptors do not work.
NASA laser communications to provide Orion faster connections
30.03.2017 | NASA/Goddard Space Flight Center
Pinball at the atomic level
30.03.2017 | Max-Planck-Institut für Struktur und Dynamik der Materie
The Institute of Semiconductor Technology and the Institute of Physical and Theoretical Chemistry, both members of the Laboratory for Emerging Nanometrology (LENA), at Technische Universität Braunschweig are partners in a new European research project entitled ChipScope, which aims to develop a completely new and extremely small optical microscope capable of observing the interior of living cells in real time. A consortium of 7 partners from 5 countries will tackle this issue with very ambitious objectives during a four-year research program.
To demonstrate the usefulness of this new scientific tool, at the end of the project the developed chip-sized microscope will be used to observe in real-time...
Astronomers from Bonn and Tautenburg in Thuringia (Germany) used the 100-m radio telescope at Effelsberg to observe several galaxy clusters. At the edges of these large accumulations of dark matter, stellar systems (galaxies), hot gas, and charged particles, they found magnetic fields that are exceptionally ordered over distances of many million light years. This makes them the most extended magnetic fields in the universe known so far.
The results will be published on March 22 in the journal „Astronomy & Astrophysics“.
Galaxy clusters are the largest gravitationally bound structures in the universe. With a typical extent of about 10 million light years, i.e. 100 times the...
Researchers at the Goethe University Frankfurt, together with partners from the University of Tübingen in Germany and Queen Mary University as well as Francis Crick Institute from London (UK) have developed a novel technology to decipher the secret ubiquitin code.
Ubiquitin is a small protein that can be linked to other cellular proteins, thereby controlling and modulating their functions. The attachment occurs in many...
In the eternal search for next generation high-efficiency solar cells and LEDs, scientists at Los Alamos National Laboratory and their partners are creating...
Silicon nanosheets are thin, two-dimensional layers with exceptional optoelectronic properties very similar to those of graphene. Albeit, the nanosheets are less stable. Now researchers at the Technical University of Munich (TUM) have, for the first time ever, produced a composite material combining silicon nanosheets and a polymer that is both UV-resistant and easy to process. This brings the scientists a significant step closer to industrial applications like flexible displays and photosensors.
Silicon nanosheets are thin, two-dimensional layers with exceptional optoelectronic properties very similar to those of graphene. Albeit, the nanosheets are...
20.03.2017 | Event News
14.03.2017 | Event News
07.03.2017 | Event News
30.03.2017 | Health and Medicine
30.03.2017 | Health and Medicine
30.03.2017 | Medical Engineering