In a paper that appeared online last week in the journal Pigment Cell & Melanoma Research, a team of UNC researchers tested whether DNA methylation profiling could be accomplished on melanoma and mole tissues that had been preserved in fixatives for typical pathology examination after biopsy.
They found that results on tissues prepared in this way were reliable and DNA methylation distinguished malignant melanomas from non-malignant moles.
Melanoma is one of the only forms of cancer that is still on the rise and is the most common form of cancer in young adults. The incidence of melanoma in women under age 30 has increased more than 50 percent since 1980.
"When melanoma is diagnosed early, the prognosis is good. However, once it spreads, it is very difficult to treat. Melanomas and moles can appear similar on the skin and under the microscope making diagnosis of some melanomas difficult. That's why we wanted to determine whether a test for DNA methylation is feasible as a tool for diagnosis," added Nancy Thomas, MD, PhD, professor of dermatology and a member of UNC Lineberger Comprehensive Cancer Center.
Kathleen Conway Dorsey, Ph.D, added, "We are very excited because, with this study, we have shown that this type of testing is feasible and that it has the potential to reliably distinguish between melanoma and benign skin lesions. Devising a molecular test that could aid in the early specific diagnosis of melanoma could have significant benefit for patients." Conway is assistant research professor of epidemiology at UNC's Gillings School of Global Public Health and a member of UNC Lineberger Comprehensive Cancer Center.
The team's research pinpointed sites on 22 genes that have significantly different methylation levels between melanomas and non-melanoma lesions, as well as 12 locations that are highly predictive of melanoma. According to Thomas, another goal of the team is to develop a DNA-methylation test for melanoma tumor DNA that is shed into the bloodstream and that can serve as a measure for disease activity.
"If this test can be developed, it opens the door to diagnose recurrence early and initiate treatment while tumors are more likely to respond to treatment. It would also give us another way to monitor patients for response to treatment and help us better optimize treatments for each patient," Thomas noted.
Other members of the research team include Sharon Edmiston, BS, Zakaria Khondker, MStat, Pamela Groben, MD, clinical professor of pathology & Laboratory Medicine, Xin Zhou, PhD, Pei Fen Kuan, PhD, research assistant professor of biostatistics, Honglin Hao, Craig Carson, PhD, and David Ollila, MD, associate professor of surgery, all at UNC-Chapel Hill. The team also included Haitao Chu, MD, PhD, of the University of Minnesota and Marianne Berwick, PhD, MPH, of the University of New Mexico.
The research was funded by the National Cancer Institute and a UNC Lineberger Pilot Grant.
Ellen de Graffenreid | EurekAlert!
Researchers identify new way to unmask melanoma cells to the immune system
17.01.2018 | Duke University Medical Center
Study advances gene therapy for glaucoma
17.01.2018 | University of Wisconsin-Madison
What enables electrons to be transferred swiftly, for example during photosynthesis? An interdisciplinary team of researchers has worked out the details of how...
For the first time, scientists have precisely measured the effective electrical charge of a single molecule in solution. This fundamental insight of an SNSF Professor could also pave the way for future medical diagnostics.
Electrical charge is one of the key properties that allows molecules to interact. Life itself depends on this phenomenon: many biological processes involve...
At the JEC World Composite Show in Paris in March 2018, the Fraunhofer Institute for Laser Technology ILT will be focusing on the latest trends and innovations in laser machining of composites. Among other things, researchers at the booth shared with the Aachen Center for Integrative Lightweight Production (AZL) will demonstrate how lasers can be used for joining, structuring, cutting and drilling composite materials.
No other industry has attracted as much public attention to composite materials as the automotive industry, which along with the aerospace industry is a driver...
Scientists at Tokyo Institute of Technology (Tokyo Tech) and Tohoku University have developed high-quality GFO epitaxial films and systematically investigated their ferroelectric and ferromagnetic properties. They also demonstrated the room-temperature magnetocapacitance effects of these GFO thin films.
Multiferroic materials show magnetically driven ferroelectricity. They are attracting increasing attention because of their fascinating properties such as...
The oceans are the largest global heat reservoir. As a result of man-made global warming, the temperature in the global climate system increases; around 90% of...
08.01.2018 | Event News
11.12.2017 | Event News
08.12.2017 | Event News
17.01.2018 | Ecology, The Environment and Conservation
17.01.2018 | Physics and Astronomy
17.01.2018 | Awards Funding