Forum for Science, Industry and Business

Sponsored by:     3M 
Search our Site:

 

Ultrasound idea: Prototype NIST/CU bioreactor evaluates engineered tissue while creating it

04.05.2012
Researchers at the National Institute of Standards and Technology (NIST) have developed a prototype bioreactor — a device for culturing cells to create engineered tissues — that both stimulates and evaluates tissue as it grows, mimicking natural processes while eliminating the need to stop periodically to cut up samples for analysis. Tissue created this way might someday be used to replace, for example, damaged or diseased cartilage in the knee and hip.

Conventional methods for evaluating the development and properties of engineered tissue are time-consuming, destructive and need to be repeated many times. By using ultrasound to monitor tissue during processing without destroying it, the novel bioreactor could be a faster and less expensive alternative.

"Most bioreactors don't do any type of nondestructive evaluation," says NIST postdoctoral researcher Jenni Popp, first author of a new paper* about the instrument. "Having some sort of ongoing evaluation of the developing tissue is definitely novel."

Cartilage is smooth connective tissue that supports joint motion. Natural cartilage is created by specialized cells that generate large amounts of structural proteins to weave a tough support material called extracellular matrix. Lacking blood vessels, cartilage has limited capability to heal from arthritis, sports injuries or other defects. Damage can be treated with drugs or joint replacement but results can be imperfect. Engineered tissue is used in some medical treatments but is not yet a routine alternative to metal or plastic joint replacements. The NIST bioreactor gives researchers a noninvasive way to monitor important structural changes in developing tissue.

The NIST/CU bioreactor can fit inside a standard incubator, which controls temperature and acidity in the growth environment. The bioreactor applies force to stimulate five small cubes of cartilage cells embedded in water-based gels. The mechanical force mimics the natural stimuli needed for the cells to create matrix proteins and develop the structure and properties of real cartilage. Ultrasound techniques monitor tissue changes over time, while a digital video microscope takes images.

Preliminary studies indicate the bioreactor both stimulates and monitors development of cells, matrix content and scaffolds to make three-dimensional engineered cartilage. The cell-laden gels were stimulated twice daily for an hour. Sulfated glycosaminoglycan (sGAG)—which combines with fibrous proteins to form the extracellular matrix—increased significantly after seven days. This structural change was detected by a significant decrease in ultrasound signals after seven days.

The research described in the new paper was performed at and led by NIST. The bioreactor is a collaborative project with several co-authors from the University of Colorado Boulder (CU) Department of Chemical and Biological Engineering.

NIST and CU researchers continue to develop ultrasonic measurement methods and plan to conduct longer experiments. The bioreactor is also being used by other academic researchers as a tool for validating mathematical models of biokinetics, the study of growth and movement in developing tissue.

* J.R. Popp, J.J. Roberts, D.V. Gallagher, K.S. Anseth, S.J. Bryant and T.P. Quinn. An instrumented bioreactor for mechanical stimulation and real-time, nondestructive evaluation of engineered cartilage tissue. Journal of Medical Devices, June 2012, Vol. 6, issue 2, 021006, posted online April 26.

Laura Ost | EurekAlert!
Further information:
http://www.nist.gov

More articles from Medical Engineering:

nachricht Virtual Reality in Medicine: New Opportunities for Diagnostics and Surgical Planning
07.12.2016 | Universität Basel

nachricht 3-D printed kidney phantoms aid nuclear medicine dosing calibration
06.12.2016 | Society of Nuclear Medicine

All articles from Medical Engineering >>>

The most recent press releases about innovation >>>

Die letzten 5 Focus-News des innovations-reports im Überblick:

Im Focus: Electron highway inside crystal

Physicists of the University of Würzburg have made an astonishing discovery in a specific type of topological insulators. The effect is due to the structure of the materials used. The researchers have now published their work in the journal Science.

Topological insulators are currently the hot topic in physics according to the newspaper Neue Zürcher Zeitung. Only a few weeks ago, their importance was...

Im Focus: Significantly more productivity in USP lasers

In recent years, lasers with ultrashort pulses (USP) down to the femtosecond range have become established on an industrial scale. They could advance some applications with the much-lauded “cold ablation” – if that meant they would then achieve more throughput. A new generation of process engineering that will address this issue in particular will be discussed at the “4th UKP Workshop – Ultrafast Laser Technology” in April 2017.

Even back in the 1990s, scientists were comparing materials processing with nanosecond, picosecond and femtosesecond pulses. The result was surprising:...

Im Focus: Shape matters when light meets atom

Mapping the interaction of a single atom with a single photon may inform design of quantum devices

Have you ever wondered how you see the world? Vision is about photons of light, which are packets of energy, interacting with the atoms or molecules in what...

Im Focus: Novel silicon etching technique crafts 3-D gradient refractive index micro-optics

A multi-institutional research collaboration has created a novel approach for fabricating three-dimensional micro-optics through the shape-defined formation of porous silicon (PSi), with broad impacts in integrated optoelectronics, imaging, and photovoltaics.

Working with colleagues at Stanford and The Dow Chemical Company, researchers at the University of Illinois at Urbana-Champaign fabricated 3-D birefringent...

Im Focus: Quantum Particles Form Droplets

In experiments with magnetic atoms conducted at extremely low temperatures, scientists have demonstrated a unique phase of matter: The atoms form a new type of quantum liquid or quantum droplet state. These so called quantum droplets may preserve their form in absence of external confinement because of quantum effects. The joint team of experimental physicists from Innsbruck and theoretical physicists from Hannover report on their findings in the journal Physical Review X.

“Our Quantum droplets are in the gas phase but they still drop like a rock,” explains experimental physicist Francesca Ferlaino when talking about the...

All Focus news of the innovation-report >>>

Anzeige

Anzeige

Event News

ICTM Conference 2017: Production technology for turbomachine manufacturing of the future

16.11.2016 | Event News

Innovation Day Laser Technology – Laser Additive Manufacturing

01.11.2016 | Event News

#IC2S2: When Social Science meets Computer Science - GESIS will host the IC2S2 conference 2017

14.10.2016 | Event News

 
Latest News

Researchers identify potentially druggable mutant p53 proteins that promote cancer growth

09.12.2016 | Life Sciences

Scientists produce a new roadmap for guiding development & conservation in the Amazon

09.12.2016 | Ecology, The Environment and Conservation

Satellites, airport visibility readings shed light on troops' exposure to air pollution

09.12.2016 | Health and Medicine

VideoLinks
B2B-VideoLinks
More VideoLinks >>>