Forum for Science, Industry and Business

Sponsored by:     3M 
Search our Site:

 

From worm to man

10.08.2011
Flatworms provide new insight into organ regeneration and the evolution of mammalian kidneys

Our bodies are perfectly capable of renewing billions of cells every day but fail miserably when it comes to replacing damaged organs such as kidneys. Using the flatworm Schmidtea mediterranea—famous for its capacity to regrow complete animals from minuscule flecks of tissue—as an eloquent example, researchers at the Stowers Institute for Medical Research demonstrated how our distant evolutionary cousins regenerate their excretory systems from scratch.


Planaria have a network of fine tubules (shown in white) running throughout their bodies. The tubules are connected to bulb-like structures called flame cells. These cells contain cilia, which move the fluids toward the excretory pores located throughout the epidermis. Credit: Courtesy of the Sanchéz Alvarado lab, Stowers Institute for Medical Research

In the process, the Stowers team led by Howard Hughes Medical Institute and Stowers investigator Alejandro Sanchéz Alvarado, Ph.D., not only established flatworms as a valuable model system to study tissue maintenance and organ regeneration but also provided new clues about the evolutionary origin of mammalian kidneys. Their study is published in the current issue of the journal Development.

"The past ten years of planaria research have shown that planarians use pretty much the same molecular toolkit for building their bodies as other animals," says first author Jochen Rink, Ph.D., formerly a postdoctoral researcher in the Sanchéz lab and now a group leader at the Max-Planck-Institute of Molecular Cell Biology and Genetics in Dresden, Germany. "Because of this fundamental similarity between all animals, understanding how a worm assembles its excretory system may very well be relevant to understanding kidney physiology and regeneration in humans," he adds.

Most animals studied in the lab fall within two main branches on the evolutionary tree of life: mice, rats, and salamanders are part of the vertebrate lineage, while fruit flies and the roundworm Ceanorhabditis elegans belong to the lineage encompassing molting animals. "People have drawn conclusions about the evolution of certain attributes by looking exclusively at these two branches," says Sanchéz Alvarado. "That's akin to trying to work out your family tree but ignoring your entire maternal lineage."

In contrast, flatworms, also known as planaria, are part of a branch that has been largely ignored by modern molecular biology. "The so called lophotrochozoa are an incredibly large and diverse group of animals we know very little about," explains Sanchéz Alvarado.

What was known is that—unlike fruit flies and C. elegans—planaria possess very complex excretory systems akin in many respects to mammalian kidneys. "Planarian protonephridia are so far the only invertebrate model system that combines pressure filtration with filtrate modification similar to mammalian nephrons, the basic functional unit of the kidney," explains Rink. Their anatomy, however, was not well understood.

When Rink and co-first author Hanh Thi-Kim Vu, Ph.D., a graduate student in the Sanchez Alvarado lab, analyzed their structure with the help of electron microscopy, the scientists found that planarian protonephridia are complex epithelial organs that organized in a consistent and hierarchical manner: a distal tubule branching out into proximal tubules, each of which is topped by a ciliated flame cell—so named because under a microscope the beating of the cilia resembles a flickering candle.

But more importantly, they noticed that the endothelia forming the tubules are composed of a fixed succession of different cell types along their length reminiscent of the mammalian condition. "This suggests to us that the mammalian kidney originated before the branches split," says Sanchéz Alvarado. "The homologous structures in fruit flies and C. elegans diversified and diverged till they no longer resembled their common ancestor."

Protonephridia are distributed throughout a flatworm's body. To study their development researchers could simply cut the animals' heads off and watch how they regrew the missing body part including excretory tubules within a week. They found that protonephridial tubules originated from a precursor structure, which undergoes extensive branching morphogenesis, the same process that also shapes vertebrate organs such as lung, kidneys or mammary glands.

When Rink interfered with the expression of EGFR5, short for epidermal growth factor receptor, during the regeneration process protonephridia could no longer undergo branching morphogenesis. If he did the same in non-regenerating planaria, they were unable to maintain the structural integrity of their protonephridia, which told him that under normal conditions the organ is constantly maintained through cell renewal.

"We take it for granted that we go to bed with two sets of fully functional kidneys and that we wake up with them the next morning but we don't understand the fundamental processes that give rise to this very well choreographed maintenance of an organism's form and function," says Sanchéz Alvarado. "We can now start to use planaria as a model to begin to understand how an adult animals maintain their form and function over a very long time."

The work was funded in part by the Howard Hughes Medical Institute and by the National Institutes of Health, National Institute of General Medical Sciences.

About the Stowers Institute for Medical Research

The Stowers Institute for Medical Research is a non-profit, basic biomedical research organization dedicated to improving human health by studying the fundamental processes of life. Jim Stowers, founder of American Century Investments, and his wife Virginia opened the Institute in 2000. Since then, the Institute has spent over a half billion dollars in pursuit of its mission.

Currently the Institute is home to nearly 500 researchers and support personnel; over 20 independent research programs; and more than a dozen technology development and core facilities. Learn more about the Institute at http://www.stowers.org.

Gina Kirchweger | EurekAlert!
Further information:
http://www.stowers.org

More articles from Life Sciences:

nachricht Asian dust providing key nutrients for California's giant sequoias
28.03.2017 | University of California - Riverside

nachricht Chlamydia: How bacteria take over control
28.03.2017 | Julius-Maximilians-Universität Würzburg

All articles from Life Sciences >>>

The most recent press releases about innovation >>>

Die letzten 5 Focus-News des innovations-reports im Überblick:

Im Focus: A Challenging European Research Project to Develop New Tiny Microscopes

The Institute of Semiconductor Technology and the Institute of Physical and Theoretical Chemistry, both members of the Laboratory for Emerging Nanometrology (LENA), at Technische Universität Braunschweig are partners in a new European research project entitled ChipScope, which aims to develop a completely new and extremely small optical microscope capable of observing the interior of living cells in real time. A consortium of 7 partners from 5 countries will tackle this issue with very ambitious objectives during a four-year research program.

To demonstrate the usefulness of this new scientific tool, at the end of the project the developed chip-sized microscope will be used to observe in real-time...

Im Focus: Giant Magnetic Fields in the Universe

Astronomers from Bonn and Tautenburg in Thuringia (Germany) used the 100-m radio telescope at Effelsberg to observe several galaxy clusters. At the edges of these large accumulations of dark matter, stellar systems (galaxies), hot gas, and charged particles, they found magnetic fields that are exceptionally ordered over distances of many million light years. This makes them the most extended magnetic fields in the universe known so far.

The results will be published on March 22 in the journal „Astronomy & Astrophysics“.

Galaxy clusters are the largest gravitationally bound structures in the universe. With a typical extent of about 10 million light years, i.e. 100 times the...

Im Focus: Tracing down linear ubiquitination

Researchers at the Goethe University Frankfurt, together with partners from the University of Tübingen in Germany and Queen Mary University as well as Francis Crick Institute from London (UK) have developed a novel technology to decipher the secret ubiquitin code.

Ubiquitin is a small protein that can be linked to other cellular proteins, thereby controlling and modulating their functions. The attachment occurs in many...

Im Focus: Perovskite edges can be tuned for optoelectronic performance

Layered 2D material improves efficiency for solar cells and LEDs

In the eternal search for next generation high-efficiency solar cells and LEDs, scientists at Los Alamos National Laboratory and their partners are creating...

Im Focus: Polymer-coated silicon nanosheets as alternative to graphene: A perfect team for nanoelectronics

Silicon nanosheets are thin, two-dimensional layers with exceptional optoelectronic properties very similar to those of graphene. Albeit, the nanosheets are less stable. Now researchers at the Technical University of Munich (TUM) have, for the first time ever, produced a composite material combining silicon nanosheets and a polymer that is both UV-resistant and easy to process. This brings the scientists a significant step closer to industrial applications like flexible displays and photosensors.

Silicon nanosheets are thin, two-dimensional layers with exceptional optoelectronic properties very similar to those of graphene. Albeit, the nanosheets are...

All Focus news of the innovation-report >>>

Anzeige

Anzeige

Event News

International Land Use Symposium ILUS 2017: Call for Abstracts and Registration open

20.03.2017 | Event News

CONNECT 2017: International congress on connective tissue

14.03.2017 | Event News

ICTM Conference: Turbine Construction between Big Data and Additive Manufacturing

07.03.2017 | Event News

 
Latest News

Researchers create artificial materials atom-by-atom

28.03.2017 | Physics and Astronomy

Researchers show p300 protein may suppress leukemia in MDS patients

28.03.2017 | Health and Medicine

Asian dust providing key nutrients for California's giant sequoias

28.03.2017 | Life Sciences

VideoLinks
B2B-VideoLinks
More VideoLinks >>>