UCSB study shows that key protein in epithelial cells plays important roles in how cells sense direction
What do sled dogs and cell clusters have in common? According to research by UC Santa Barbara’s Denise Montell, they both travel in groups and need a leader to make sure they all follow in the same direction.
An optical sensor of mechanical tension (blue) inserted between E-cadherin (green).
Photo Credit: Illustration by Peter Allen
Montell, Duggan Professor of Molecular Cellular and Developmental Biology, and colleagues worked on three independent projects involving E-cadherin, a protein found in epithelial cells throughout the body. The researchers used fruit-fly ovaries to uncover the role played by E-cadherin in collective cell migration. Their findings are reported today in the journal Cell.
According to traditional scientific dogma, E-cadherin acts like the mortar between bricks, holding cells together and preventing motility. Montell’s team found the opposite: Cadherin is actually promoting the ability of cells to move and migrate. “It’s doing it in three different ways in three different parts of the cell,” Montell said. “In each spot in the cell, cadherin is doing something different and all of those function together to orchestrate the movement of cells.”
Montell’s team sought to understand the E-cadherin-mediated migration of these cells with three separate approaches that converged on one idea: the guidance of cell movement. “This kind of motility is similar to what tumor cells do when they metastasize,” Montell said, “and it’s part of the normal development of different tissues.”
One researcher studied the interaction between the lead cell and the following cells; hence, the sled-dog analogy. The lead cell is tethered by E-caderin to other cells in the cluster and pulls them in the proper direction in the same way the lead sled dog guides its team.
While E-cadherin is distributed throughout the cell cluster, the lead cell — determined by the strong force of E-cadherin that causes the cell to protrude in the direction of the movement — can communicate with the side and rear cells and prohibit them from protruding. This creates a front and back to the entire cluster of cells.
Lead author Danfeng Cai, a graduate student in the Denise Montell Lab, suppressed cadherin in different cell types and analyzed the visible guidance defects by making movies of the migrating cells. When cells lacked E-cadherin, they were unable to migrate as an organized group, and the direction of their movement was random and irregular.
Another member of the research team designed an optical sensor of mechanical tension — a kind of microscopic spring — and inserted it into the cadherin protein. This enabled the measurement of force on the E-cadherin molecules. The results showed that even though the distribution of the protein was uniform, the force per molecule was stronger at the front. “It’s kind of amazing that you can make that measurement in living cells,” Montell said.
In addition to highlighting E-cadherin’s role in cell migration, the paper introduces new experimental tools the team used to probe and analyze E-cadherin in living tissue, the first being the optical sensor of mechanical tension. The researchers found that E-cadherin molecules implanted with the sensor were fully functional. This allowed them to generate transgenic flies containing only E-cadherin molecules with optical sensors.
“This in vivo tension sensor could revolutionize the area of research that strives to elucidate the interplay between biochemical signals and mechanical forces during morphogenesis,” Montell said. “In contrast to other approaches that attempt to measure forces in tissues, such as laser cutting, this one is nonperturbing.”
A second tool Montell’s team developed and reported for the first time is morphodynamic profiling. This quantitative approach allowed the researchers to compare changing cell morphologies over time between different genotypes.
Data from the cell movement imaging were mathematically translated into graphs showing cell protrusion and retraction velocities at different points in time. An analysis of 26 parameters showed that the E-cadherin and the chemoattractant receptors thought to be the guiding cell movement had statistically indistinguishable phenotypes, indicating that E-cadherin and the classic guidance receptors function in the same pathway.
“E-cadherin is serving multiple purposes,” Montell concluded, “all of which function together to coordinate the collective ability of these cells to sense direction. Our work demonstrates three completely different approaches that all show the same result.” While E-cadherin serves different functions in different subcellular locations, it appears to drive cohesive cell migration, an entirely new role for this much-studied molecule.
Julie Cohen | Eurek Alert!
Symbiotic bacteria: from hitchhiker to beetle bodyguard
28.04.2017 | Johannes Gutenberg-Universität Mainz
Nose2Brain – Better Therapy for Multiple Sclerosis
28.04.2017 | Fraunhofer-Institut für Grenzflächen- und Bioverfahrenstechnik IGB
More and more automobile companies are focusing on body parts made of carbon fiber reinforced plastics (CFRP). However, manufacturing and repair costs must be further reduced in order to make CFRP more economical in use. Together with the Volkswagen AG and five other partners in the project HolQueSt 3D, the Laser Zentrum Hannover e.V. (LZH) has developed laser processes for the automatic trimming, drilling and repair of three-dimensional components.
Automated manufacturing processes are the basis for ultimately establishing the series production of CFRP components. In the project HolQueSt 3D, the LZH has...
Reflecting the structure of composites found in nature and the ancient world, researchers at the University of Illinois at Urbana-Champaign have synthesized thin carbon nanotube (CNT) textiles that exhibit both high electrical conductivity and a level of toughness that is about fifty times higher than copper films, currently used in electronics.
"The structural robustness of thin metal films has significant importance for the reliable operation of smart skin and flexible electronics including...
The nearby, giant radio galaxy M87 hosts a supermassive black hole (BH) and is well-known for its bright jet dominating the spectrum over ten orders of magnitude in frequency. Due to its proximity, jet prominence, and the large black hole mass, M87 is the best laboratory for investigating the formation, acceleration, and collimation of relativistic jets. A research team led by Silke Britzen from the Max Planck Institute for Radio Astronomy in Bonn, Germany, has found strong indication for turbulent processes connecting the accretion disk and the jet of that galaxy providing insights into the longstanding problem of the origin of astrophysical jets.
Supermassive black holes form some of the most enigmatic phenomena in astrophysics. Their enormous energy output is supposed to be generated by the...
The probability to find a certain number of photons inside a laser pulse usually corresponds to a classical distribution of independent events, the so-called...
Microprocessors based on atomically thin materials hold the promise of the evolution of traditional processors as well as new applications in the field of flexible electronics. Now, a TU Wien research team led by Thomas Müller has made a breakthrough in this field as part of an ongoing research project.
Two-dimensional materials, or 2D materials for short, are extremely versatile, although – or often more precisely because – they are made up of just one or a...
28.04.2017 | Event News
20.04.2017 | Event News
18.04.2017 | Event News
28.04.2017 | Medical Engineering
28.04.2017 | Earth Sciences
28.04.2017 | Life Sciences