Forum for Science, Industry and Business

Sponsored by:     3M 
Search our Site:

 

What fuels Salmonella's invasion strategy?

06.05.2014

Certain strains of Salmonella bacteria such as Salmonella Typhimurium (S. Typhimurium) are among of the most common causes of food-borne gastroenteritis.

Other strains of Salmonella such as S. Typhi are responsible for typhoid fever, which causes 200,000 deaths around the world each year. Ensuring food is clear of contamination, and water is clean are key to reducing the effects Salmonella can have, but we also need more effective ways to combat Salmonella once it's inside our bodies.


This is Salmonella Typhimurium.

Credit: Institute of Food Research

To address this the Institute of Food Research, strategically supported by the Biotechnology and Biological Sciences Research Council, has been studying S. Typhimurium bacteria to understand, not only how they transmit through the food chain, but why they are so effective and dangerous once inside us.

If we consume food or water contaminated with S. Typhimurium, the first stage of infection is to get into the cells that line our gut. These epithelial cells are adapted to defend against such attacks, but Salmonella has a wealth of strategies to overcome these and make it more virulent. It also needs these virulence genes to overcome the cells of the immune system, which it invades to move around the body.

We are learning a lot about these virulence genes, but until this new study, published in the journal PLOS ONE, we didn't know how Salmonella fuelled itself for this. A source of energy and nutrition is vital, and knowing what Salmonella uses could inform new strategies to prevent infection.

To discover more about Salmonella's feeding habits, Dr Arthur Thompson and his team constructed S. Typhimurium strains lacking certain key genes in important metabolic pathways. They then examined how well these mutated strains reproduced in human epithelial cells, grown in cultures.

"We found that glucose is the major nutrient used by S. Typhimurium," said Dr Thompson. Salmonella converts glucose to pyruvate in a process called glycolysis, which also releases energy needed to fuel growth and reproduction.

Knocking out one enzyme in glycolysis, and enzymes used to transport glucose into the bacteria severely reduced S. Typhimurium's ability to reproduce in epithelial cells, but didn't eradicate it completely. "This suggests that although S. Typhimurium requires glucose, it is also able to use other nutrients, and that's something we're now studying," said Dr Thompson.

This contrasts with previous findings from similar experiments on macrophage cells by the IFR team, as for successful macrophage invasion, glycolysis is absolutely essential. Macrophages are the immune cells sent to destroy Salmonella, but instead Salmonella invades the macrophages. Infected macrophages can carry Salmonella around the body causing a potentially fatal systemic infection.

"We now have a much more complete picture of the nutritional needs of Salmonella, which is important since this information may also suggest new ways to develop potential therapeutic interventions," said Dr Thompson.

Andrew Chapple | Eurek Alert!
Further information:
http://www.ifr.ac.uk

More articles from Life Sciences:

nachricht Programming cells with computer-like logic
27.07.2017 | Wyss Institute for Biologically Inspired Engineering at Harvard

nachricht Identified the component that allows a lethal bacteria to spread resistance to antibiotics
27.07.2017 | Institute for Research in Biomedicine (IRB Barcelona)

All articles from Life Sciences >>>

The most recent press releases about innovation >>>

Die letzten 5 Focus-News des innovations-reports im Überblick:

Im Focus: Physicists Design Ultrafocused Pulses

Physicists working with researcher Oriol Romero-Isart devised a new simple scheme to theoretically generate arbitrarily short and focused electromagnetic fields. This new tool could be used for precise sensing and in microscopy.

Microwaves, heat radiation, light and X-radiation are examples for electromagnetic waves. Many applications require to focus the electromagnetic fields to...

Im Focus: Carbon Nanotubes Turn Electrical Current into Light-emitting Quasi-particles

Strong light-matter coupling in these semiconducting tubes may hold the key to electrically pumped lasers

Light-matter quasi-particles can be generated electrically in semiconducting carbon nanotubes. Material scientists and physicists from Heidelberg University...

Im Focus: Flexible proximity sensor creates smart surfaces

Fraunhofer IPA has developed a proximity sensor made from silicone and carbon nanotubes (CNT) which detects objects and determines their position. The materials and printing process used mean that the sensor is extremely flexible, economical and can be used for large surfaces. Industry and research partners can use and further develop this innovation straight away.

At first glance, the proximity sensor appears to be nothing special: a thin, elastic layer of silicone onto which black square surfaces are printed, but these...

Im Focus: 3-D scanning with water

3-D shape acquisition using water displacement as the shape sensor for the reconstruction of complex objects

A global team of computer scientists and engineers have developed an innovative technique that more completely reconstructs challenging 3D objects. An ancient...

Im Focus: Manipulating Electron Spins Without Loss of Information

Physicists have developed a new technique that uses electrical voltages to control the electron spin on a chip. The newly-developed method provides protection from spin decay, meaning that the contained information can be maintained and transmitted over comparatively large distances, as has been demonstrated by a team from the University of Basel’s Department of Physics and the Swiss Nanoscience Institute. The results have been published in Physical Review X.

For several years, researchers have been trying to use the spin of an electron to store and transmit information. The spin of each electron is always coupled...

All Focus news of the innovation-report >>>

Anzeige

Anzeige

Event News

Clash of Realities 2017: Registration now open. International Conference at TH Köln

26.07.2017 | Event News

Closing the Sustainability Circle: Protection of Food with Biobased Materials

21.07.2017 | Event News

»We are bringing Additive Manufacturing to SMEs«

19.07.2017 | Event News

 
Latest News

Programming cells with computer-like logic

27.07.2017 | Life Sciences

Identified the component that allows a lethal bacteria to spread resistance to antibiotics

27.07.2017 | Life Sciences

Malaria Already Endemic in the Mediterranean by the Roman Period

27.07.2017 | Health and Medicine

VideoLinks
B2B-VideoLinks
More VideoLinks >>>