Forum for Science, Industry and Business

Sponsored by:     3M 
Search our Site:

 

New technique elucidates dynamics of plant cell metabolites

14.09.2011
A new technique developed by researchers at RIKEN has clarified the location and dynamics of specific metabolites in a single cell of the alga Chara australis. The findings reveal that these metabolites are regulated and fluctuate under stress conditions, providing insight into previously-unknown functions of the vacuole in cellular processes.

Metabolites, the intermediates and products of chemical reactions that sustain all living organisms, play a central role in cellular processes including growth, differentiation and defense. Despite their importance, however, our understanding of the role of these metabolites in the cell is incomplete due to the lack of techniques for analyzing them at high spatial resolution.

In a paper to appear in the Journal Plant Physiology, researchers at the RIKEN Plant Science Center (PSC) in Yokohama, Japan describe a novel technique which enables such high-resolution metabolite analysis. Their research focuses on Chara australis, an alga whose single, extremely large (up to 20cm long) internodal cell provides a unique opportunity to study the detailed location and dynamics of metabolites inside the cell.

Their technique separates the cell into two parts, isolating its cytoplasm (containing plastids, mitochondria, nuclei, endomembrane system and cell wall) from its vacuole, a large water-filled compartment containing organic and inorganic molecules. Using this technique, the researchers demonstrate that the concentrations of 125 known metabolites in the vacuole and cytoplasm fluctuate asynchronously under stress conditions, suggesting that metabolites are spatially regulated in the cell.

By shedding light on the detailed distribution of metabolites in the cell, this finding marks a major advance in our understanding of plant cell metabolism. The technique used likewise charts new ground, providing unprecedented detail on organelle-specific metabolite concentration and highlighting the usefulness of C. autralis as a model organism for biological studies at the single-cell level.

For more information, please contact:

Akira Oikawa
Metabolomic Function Research Group (Tsuruoka)
RIKEN Plant Science Center
Tel: +81-(0)235-25-3580 / Fax: +81-(0)235-25-3580
Kazuki Saito
Metabolomic Function Research Group
RIKEN Plant Science Center
Tel: +81-(0)45-503-9488 / Fax: +81-(0)45-503-9489
Global Relations Office
RIKEN
Tel: +81-(0)48-462-1225 / Fax: +81-(0)48-463-3687
Email: koho@riken.jp
Reach us on Twitter: @rikenresearch
Reference
Akira Oikawa, Fumio Matsuda, Munehiro Kikuyama, Tetsuro Mimura and Kazuki Saito, "Metabolomics of a single vacuole reveals metabolic dynamism in an alga Chara australis." Plant Physiology, 2011, DOI: 10.1104/pp.111.183772

About RIKEN

RIKEN is Japan’s flagship research institute devoted to basic and applied research. Over 2500 papers by RIKEN researchers are published every year in reputable scientific and technical journals, covering topics ranging across a broad spectrum of disciplines including physics, chemistry, biology, medical science and engineering. RIKEN’s advanced research environment and strong emphasis on interdisciplinary collaboration has earned itself an unparalleled reputation for scientific excellence in Japan and around the world.

About the Plant Science Center

With rapid industrialization and a world population set to top 9 billion within the next 30 years, the need to increase our food production capacity is more urgent today than it ever has been before. Avoiding a global crisis demands rapid advances in plant science research to boost crop yields and ensure a reliable supply of food, energy and plant-based materials.

The RIKEN Plant Science Center (PSC), located at the RIKEN Yokohama Institute in Yokohama City, Japan, is at the forefront of research efforts to uncover mechanisms underlying plant metabolism, morphology and development, and apply these findings to improving plant production. With laboratories ranging in subject area from metabolomics, to functional genomics, to plant regulation and productivity, to plant evolution and adaptation, the PSC's broad scope grants it a unique position in the network of modern plant science research. In cooperation with universities, research institutes and industry, the PSC is working to ensure a stable supply of food, materials, and energy to support a growing world population and its pressing health and environmental needs.

gro-pr | Research asia research news
Further information:
http://www.riken.jp
http://www.researchsea.com

More articles from Life Sciences:

nachricht How brains surrender to sleep
23.06.2017 | IMP - Forschungsinstitut für Molekulare Pathologie GmbH

nachricht A new technique isolates neuronal activity during memory consolidation
22.06.2017 | Spanish National Research Council (CSIC)

All articles from Life Sciences >>>

The most recent press releases about innovation >>>

Die letzten 5 Focus-News des innovations-reports im Überblick:

Im Focus: Can we see monkeys from space? Emerging technologies to map biodiversity

An international team of scientists has proposed a new multi-disciplinary approach in which an array of new technologies will allow us to map biodiversity and the risks that wildlife is facing at the scale of whole landscapes. The findings are published in Nature Ecology and Evolution. This international research is led by the Kunming Institute of Zoology from China, University of East Anglia, University of Leicester and the Leibniz Institute for Zoo and Wildlife Research.

Using a combination of satellite and ground data, the team proposes that it is now possible to map biodiversity with an accuracy that has not been previously...

Im Focus: Climate satellite: Tracking methane with robust laser technology

Heatwaves in the Arctic, longer periods of vegetation in Europe, severe floods in West Africa – starting in 2021, scientists want to explore the emissions of the greenhouse gas methane with the German-French satellite MERLIN. This is made possible by a new robust laser system of the Fraunhofer Institute for Laser Technology ILT in Aachen, which achieves unprecedented measurement accuracy.

Methane is primarily the result of the decomposition of organic matter. The gas has a 25 times greater warming potential than carbon dioxide, but is not as...

Im Focus: How protons move through a fuel cell

Hydrogen is regarded as the energy source of the future: It is produced with solar power and can be used to generate heat and electricity in fuel cells. Empa researchers have now succeeded in decoding the movement of hydrogen ions in crystals – a key step towards more efficient energy conversion in the hydrogen industry of tomorrow.

As charge carriers, electrons and ions play the leading role in electrochemical energy storage devices and converters such as batteries and fuel cells. Proton...

Im Focus: A unique data centre for cosmological simulations

Scientists from the Excellence Cluster Universe at the Ludwig-Maximilians-Universität Munich have establised "Cosmowebportal", a unique data centre for cosmological simulations located at the Leibniz Supercomputing Centre (LRZ) of the Bavarian Academy of Sciences. The complete results of a series of large hydrodynamical cosmological simulations are available, with data volumes typically exceeding several hundred terabytes. Scientists worldwide can interactively explore these complex simulations via a web interface and directly access the results.

With current telescopes, scientists can observe our Universe’s galaxies and galaxy clusters and their distribution along an invisible cosmic web. From the...

Im Focus: Scientists develop molecular thermometer for contactless measurement using infrared light

Temperature measurements possible even on the smallest scale / Molecular ruby for use in material sciences, biology, and medicine

Chemists at Johannes Gutenberg University Mainz (JGU) in cooperation with researchers of the German Federal Institute for Materials Research and Testing (BAM)...

All Focus news of the innovation-report >>>

Anzeige

Anzeige

Event News

Plants are networkers

19.06.2017 | Event News

Digital Survival Training for Executives

13.06.2017 | Event News

Global Learning Council Summit 2017

13.06.2017 | Event News

 
Latest News

Quantum thermometer or optical refrigerator?

23.06.2017 | Physics and Astronomy

A 100-year-old physics problem has been solved at EPFL

23.06.2017 | Physics and Astronomy

Equipping form with function

23.06.2017 | Information Technology

VideoLinks
B2B-VideoLinks
More VideoLinks >>>