Forum for Science, Industry and Business

Sponsored by:     3M 
Search our Site:

 

Small-molecule inhibitor uncovers protein role in melanoma cell migration

16.08.2010
A nuclear protein of previously unknown function has been shown to regulate the migration of tumor cells in the spread of melanoma, the most deadly form of skin cancer.

Researchers at the RIKEN Advanced Science Institute made the discovery by means of a small-molecule inhibitor they identified using a powerful new chemical array screening technique.

Characterizing the functions of proteins in the cell, whose role in mediating complex metabolic and signaling networks is central to cellular biochemistry, is essential for developing new medicines and treatments. Small-molecule inhibitors have proven an effective tool for doing this, binding to target proteins and disrupting their normal function in order to reveal their network of cellular interactions.

Human pirin is a nuclear protein known to play a role in a variety of biological processes, yet one with a function which remains unclear. To identify inhibitors for this protein, the team used a technique they developed called chemical array screening, in which many small-compound molecules are immobilized onto glass slides and incubated with the target protein. From more than 20,000 molecules screened, the team identified one they named triphenyl compound A (TPh A) that binds to pirin with high affinity.

Using X-ray crystallography, the team determined how TPh A binds to pirin at a resolution of 2.35 Å. They went on to show that TPh A inhibits interaction between pirin and its binding partner, Bcl3, and that it also inhibits the migration of melanoma cells by reducing expression of the tumor mobility protein SNAI2.

Reported in Nature Chemical Biology, the findings establish for the first time the role of pirin in melanoma cell migration and elucidate its structure through its binding with TPh A. They also demonstrate the power of chemical array screening, whose further application promises to greatly expand our understanding of proteins and their interactions in the cell.

For more information, please contact:

Dr. Hiroyuki Osada
Chemical Biology Core Facility, Advanced Computational Sciences Department
RIKEN Advanced Science Institute
Tel: +81-(0)48-467-9542 / Fax: +81-(0)48-467-4669
Ms. Tomoko Ikawa (PI officer)
Global Relations Office
RIKEN
Tel: +81-(0)48-462-1225 / Fax: +81-(0)48-463-3687
Email: koho@riken.jp
Reference
Isao Miyazaki, Siro Simizu, Hideo Okumura, Satoshi Takagi and Hiroyuki Osada. A small-molecule inhibitor shows that pirin regulates migration of melanoma cells. Nature Chemical Biology (2010).

gro-pr | Research asia research news
Further information:
http://www.riken.jp
http://www.researchsea.com

More articles from Life Sciences:

nachricht Researchers uncover protein-based “cancer signature”
05.12.2016 | Universität Basel

nachricht The Nagoya Protocol Creates Disadvantages for Many Countries when Applied to Microorganisms
05.12.2016 | Leibniz-Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH

All articles from Life Sciences >>>

The most recent press releases about innovation >>>

Die letzten 5 Focus-News des innovations-reports im Überblick:

Im Focus: Shape matters when light meets atom

Mapping the interaction of a single atom with a single photon may inform design of quantum devices

Have you ever wondered how you see the world? Vision is about photons of light, which are packets of energy, interacting with the atoms or molecules in what...

Im Focus: Novel silicon etching technique crafts 3-D gradient refractive index micro-optics

A multi-institutional research collaboration has created a novel approach for fabricating three-dimensional micro-optics through the shape-defined formation of porous silicon (PSi), with broad impacts in integrated optoelectronics, imaging, and photovoltaics.

Working with colleagues at Stanford and The Dow Chemical Company, researchers at the University of Illinois at Urbana-Champaign fabricated 3-D birefringent...

Im Focus: Quantum Particles Form Droplets

In experiments with magnetic atoms conducted at extremely low temperatures, scientists have demonstrated a unique phase of matter: The atoms form a new type of quantum liquid or quantum droplet state. These so called quantum droplets may preserve their form in absence of external confinement because of quantum effects. The joint team of experimental physicists from Innsbruck and theoretical physicists from Hannover report on their findings in the journal Physical Review X.

“Our Quantum droplets are in the gas phase but they still drop like a rock,” explains experimental physicist Francesca Ferlaino when talking about the...

Im Focus: MADMAX: Max Planck Institute for Physics takes up axion research

The Max Planck Institute for Physics (MPP) is opening up a new research field. A workshop from November 21 - 22, 2016 will mark the start of activities for an innovative axion experiment. Axions are still only purely hypothetical particles. Their detection could solve two fundamental problems in particle physics: What dark matter consists of and why it has not yet been possible to directly observe a CP violation for the strong interaction.

The “MADMAX” project is the MPP’s commitment to axion research. Axions are so far only a theoretical prediction and are difficult to detect: on the one hand,...

Im Focus: Molecules change shape when wet

Broadband rotational spectroscopy unravels structural reshaping of isolated molecules in the gas phase to accommodate water

In two recent publications in the Journal of Chemical Physics and in the Journal of Physical Chemistry Letters, researchers around Melanie Schnell from the Max...

All Focus news of the innovation-report >>>

Anzeige

Anzeige

Event News

ICTM Conference 2017: Production technology for turbomachine manufacturing of the future

16.11.2016 | Event News

Innovation Day Laser Technology – Laser Additive Manufacturing

01.11.2016 | Event News

#IC2S2: When Social Science meets Computer Science - GESIS will host the IC2S2 conference 2017

14.10.2016 | Event News

 
Latest News

IHP presents the fastest silicon-based transistor in the world

05.12.2016 | Power and Electrical Engineering

InLight study: insights into chemical processes using light

05.12.2016 | Materials Sciences

High-precision magnetic field sensing

05.12.2016 | Power and Electrical Engineering

VideoLinks
B2B-VideoLinks
More VideoLinks >>>