Forum for Science, Industry and Business

Sponsored by:     3M 
Search our Site:


Small-molecule inhibitor uncovers protein role in melanoma cell migration

A nuclear protein of previously unknown function has been shown to regulate the migration of tumor cells in the spread of melanoma, the most deadly form of skin cancer.

Researchers at the RIKEN Advanced Science Institute made the discovery by means of a small-molecule inhibitor they identified using a powerful new chemical array screening technique.

Characterizing the functions of proteins in the cell, whose role in mediating complex metabolic and signaling networks is central to cellular biochemistry, is essential for developing new medicines and treatments. Small-molecule inhibitors have proven an effective tool for doing this, binding to target proteins and disrupting their normal function in order to reveal their network of cellular interactions.

Human pirin is a nuclear protein known to play a role in a variety of biological processes, yet one with a function which remains unclear. To identify inhibitors for this protein, the team used a technique they developed called chemical array screening, in which many small-compound molecules are immobilized onto glass slides and incubated with the target protein. From more than 20,000 molecules screened, the team identified one they named triphenyl compound A (TPh A) that binds to pirin with high affinity.

Using X-ray crystallography, the team determined how TPh A binds to pirin at a resolution of 2.35 Å. They went on to show that TPh A inhibits interaction between pirin and its binding partner, Bcl3, and that it also inhibits the migration of melanoma cells by reducing expression of the tumor mobility protein SNAI2.

Reported in Nature Chemical Biology, the findings establish for the first time the role of pirin in melanoma cell migration and elucidate its structure through its binding with TPh A. They also demonstrate the power of chemical array screening, whose further application promises to greatly expand our understanding of proteins and their interactions in the cell.

For more information, please contact:

Dr. Hiroyuki Osada
Chemical Biology Core Facility, Advanced Computational Sciences Department
RIKEN Advanced Science Institute
Tel: +81-(0)48-467-9542 / Fax: +81-(0)48-467-4669
Ms. Tomoko Ikawa (PI officer)
Global Relations Office
Tel: +81-(0)48-462-1225 / Fax: +81-(0)48-463-3687
Isao Miyazaki, Siro Simizu, Hideo Okumura, Satoshi Takagi and Hiroyuki Osada. A small-molecule inhibitor shows that pirin regulates migration of melanoma cells. Nature Chemical Biology (2010).

gro-pr | Research asia research news
Further information:

More articles from Life Sciences:

nachricht Novel mechanisms of action discovered for the skin cancer medication Imiquimod
21.10.2016 | Technische Universität München

nachricht Second research flight into zero gravity
21.10.2016 | Universität Zürich

All articles from Life Sciences >>>

The most recent press releases about innovation >>>

Die letzten 5 Focus-News des innovations-reports im Überblick:

Im Focus: New 3-D wiring technique brings scalable quantum computers closer to reality

Researchers from the Institute for Quantum Computing (IQC) at the University of Waterloo led the development of a new extensible wiring technique capable of controlling superconducting quantum bits, representing a significant step towards to the realization of a scalable quantum computer.

"The quantum socket is a wiring method that uses three-dimensional wires based on spring-loaded pins to address individual qubits," said Jeremy Béjanin, a PhD...

Im Focus: Scientists develop a semiconductor nanocomposite material that moves in response to light

In a paper in Scientific Reports, a research team at Worcester Polytechnic Institute describes a novel light-activated phenomenon that could become the basis for applications as diverse as microscopic robotic grippers and more efficient solar cells.

A research team at Worcester Polytechnic Institute (WPI) has developed a revolutionary, light-activated semiconductor nanocomposite material that can be used...

Im Focus: Diamonds aren't forever: Sandia, Harvard team create first quantum computer bridge

By forcefully embedding two silicon atoms in a diamond matrix, Sandia researchers have demonstrated for the first time on a single chip all the components needed to create a quantum bridge to link quantum computers together.

"People have already built small quantum computers," says Sandia researcher Ryan Camacho. "Maybe the first useful one won't be a single giant quantum computer...

Im Focus: New Products - Highlights of COMPAMED 2016

COMPAMED has become the leading international marketplace for suppliers of medical manufacturing. The trade fair, which takes place every November and is co-located to MEDICA in Dusseldorf, has been steadily growing over the past years and shows that medical technology remains a rapidly growing market.

In 2016, the joint pavilion by the IVAM Microtechnology Network, the Product Market “High-tech for Medical Devices”, will be located in Hall 8a again and will...

Im Focus: Ultra-thin ferroelectric material for next-generation electronics

'Ferroelectric' materials can switch between different states of electrical polarization in response to an external electric field. This flexibility means they show promise for many applications, for example in electronic devices and computer memory. Current ferroelectric materials are highly valued for their thermal and chemical stability and rapid electro-mechanical responses, but creating a material that is scalable down to the tiny sizes needed for technologies like silicon-based semiconductors (Si-based CMOS) has proven challenging.

Now, Hiroshi Funakubo and co-workers at the Tokyo Institute of Technology, in collaboration with researchers across Japan, have conducted experiments to...

All Focus news of the innovation-report >>>



Event News

#IC2S2: When Social Science meets Computer Science - GESIS will host the IC2S2 conference 2017

14.10.2016 | Event News

Agricultural Trade Developments and Potentials in Central Asia and the South Caucasus

14.10.2016 | Event News

World Health Summit – Day Three: A Call to Action

12.10.2016 | Event News

Latest News

Resolving the mystery of preeclampsia

21.10.2016 | Health and Medicine

Stanford researchers create new special-purpose computer that may someday save us billions

21.10.2016 | Information Technology

From ancient fossils to future cars

21.10.2016 | Materials Sciences

More VideoLinks >>>