Forum for Science, Industry and Business

Sponsored by:     3M 
Search our Site:

 

Scientists uncover mechanism by which plants inherit epigenetic modifications

21.09.2012
Small RNAs guide epigenetic modifications of DNA that lead to genome reprogramming

Cold Spring Harbor, N.Y. – During embryonic development in humans and other mammals, sperm and egg cells are essentially wiped clean of chemical modifications to DNA called epigenetic marks. They are then held in reserve to await fertilization.

In flowering plants the scenario is dramatically different. Germ cells don't even appear until the post-embryonic period – sometimes not until many years later. When they do appear, only some epigenetic marks are wiped away; some remain, carried over from prior generations – although until now little was known about how or to what extent.

"What we did know," says Professor and HHMI-GBMF Investigator Rob Martienssen, Ph.D., of Cold Spring Harbor Laboratory (CSHL), "was that epigenetic inheritance – the inheritance by offspring of chemical "tags" present in parental DNA that modify the expression of genes – is much more widespread in plants than in animals."

In new research published online today in the journal Cell, Martienssen and colleagues show that genome reprogramming through these epigenetic mechanisms is guided by small RNAs and is passed on to the next generation.

Some DNA is tagged with epigenetic marks

It has long been known that in plants, as the male germline pollen grains develop, they give rise to two sperm cells, and a structure called the vegetative nucleus, also known as the "nurse cell" because it provides energy and nourishment to the sperm cells.

The DNA in germ cells can exist in two dramatically different states: in one, it is very densely packed and essentially inaccessible to the cellular machinery that enables individual genes to be "expressed." In the other, in which the packing is much looser, genes can be expressed. In the latter state, because the genetic material is accessible, it is can also be modified by various chemical groups (two common ones are methyl and acetyl) which tend to attach to the DNA at specific locations.

These chemical tags are called epigenetic marks. The attachment of, for instance, a methyl group to a particular stretch of DNA containing a gene tends to prevent that gene from being accessed by the gene-expression machinery, and thus prevents the gene from being expressed.

Inherited methylation patterns are guided by small RNAs

Probing further into the set of modifications on the DNA in plant pollen grains, Martienssen and colleagues decided to look at the particular set of chemical marks called methyl groups. When they separated out pollen grains in different stages of development they found distinct patterns of the attachment of methyl groups to DNA.

They also noticed the corresponding accumulation of small RNAs, including two classes of so-called short-interfering RNAs (siRNAs) – tiny RNA molecules, 21 or 24 nucleotides in length -- involved in silencing gene expression. These small siRNAs act as guides to where methylation will occur, silencing gene expression.

Previous work by the Martienssen lab and their collaborators, including a team of pollen specialists from the Instituto Gulbenkian de Ciencia in Lisbon, Portugal, has shown that these epigenetic mechanisms are important for keeping transposons in check. Also known as "jumping genes" for their ability to be expressed and then re-insert themselves at random into a different area of the genome, transposons are dangerous because they can cause damage to DNA and disrupt genetic function.

In the current study, Martienssen's team discovered that while in sperm, some areas of DNA containing transposons had "lost" methyl groups, and thus had the potential to be expressed, the same stretches of DNA were observed to be methylated in the seed embryo. This was associated with the accumulation of 21 nucleotide long siRNA in the mature pollen and 24 nucelotide long siRNA in the seed embryo. Martienssen speculates that the loss of methylation in the sperm and subsequent re-methylation during fertilization may reflect an ancient mechanism for transposon recognition and silencing.

A second important observation made by the team was of the loss of methylation in "nurse cells." Methylation at these same sites is retained in the associated sperm cells, and, too, is associated with accumulation of 24 nucleotide siRNA. This process results in areas of recurrent epigenetic marking that are pre-methylated in the germline sperm and carried on to the next generation.

"This is what, at least in part, enables plants to inherit acquired traits from prior generations – something that we mammals can rarely do," Martienssen observes.

Being able to trace the inheritance of traits – both wanted and unwanted -- in plants, and notably in agricultural crops, is important for farmers. Martienssen predicts that "defining inheritance through epigenetic modifications will influence the ways people think about cross-breeding to select for desired traits." Such traits as resistance to temperature variation in crops have important agricultural and economic implications.

"Reprogramming of DNA Methylation in Pollen Guides Epigenetic Inheritance via Small RNA" is published online in Cell on September 20, 2012. The authors are: Joseph P. Calarco, Filipe Borges, Mark T Donoghue, Frédéric Van Ex, Pauline E Jullien, Telma Lopes, Rui Gardner, Frédéric Berger, José A Feijó, Jörg D Becker, Robert A Martienssen. The paper can be obtained online at Cell press.

The research described in this release was supported by the following grants and funding agencies: NIH grant R01 GM067014 to R.A.M., grants PTDC/AGR-GPL/103778/2008 and PTDC/BIA-BCM/103787/2008 from Fundação para a Ciência e a Tecnologia (FCT) to J.D.B. and FCT grant PTDC/BIA-BCM/108044/2008 to J.A.F. F. Berger and P.E.J. were supported by Temasek Lifescience Laboratory. J.P.C. was supported by a graduate student fellowship from NSERC and by a grant from the Fred C. Gloeckner Foundation, F. Borges was supported by a FCT PhD fellowship SFRH/BD/48761/2008, and F.V.E. was funded by a Herbert Hoover postdoctoral fellowship from the Belgian American Educational Foundation.

About Cold Spring Harbor Laboratory

Founded in 1890, Cold Spring Harbor Laboratory (CSHL) has shaped contemporary biomedical research and education with programs in cancer, neuroscience, plant biology and quantitative biology. CSHL is ranked number one in the world by Thomson Reuters for impact of its research in molecular biology and genetics. The Laboratory has been home to eight Nobel Prize winners. Today, CSHL's multidisciplinary scientific community is more than 360 scientists strong and its Meetings & Courses program hosts more than 12,500 scientists from around the world each year to its Long Island campus and its China center. Tens of thousands more benefit from the research, reviews, and ideas published in journals and books distributed internationally by CSHL Press. The Laboratory's education arm also includes a graduate school and programs for undergraduates as well as middle and high school students and teachers. CSHL is a private, not-for-profit institution on the north shore of Long Island. For more information, visit www.cshl.edu.

Written by: Edward Brydon, Science Writer | ebrydon@cshl.edu | 516-367-6822

Edward Brydon | EurekAlert!
Further information:
http://www.cshl.edu

More articles from Life Sciences:

nachricht A novel socio-ecological approach helps identifying suitable wolf habitats
17.02.2017 | Universität Zürich

nachricht New, ultra-flexible probes form reliable, scar-free integration with the brain
16.02.2017 | University of Texas at Austin

All articles from Life Sciences >>>

The most recent press releases about innovation >>>

Die letzten 5 Focus-News des innovations-reports im Überblick:

Im Focus: Breakthrough with a chain of gold atoms

In the field of nanoscience, an international team of physicists with participants from Konstanz has achieved a breakthrough in understanding heat transport

In the field of nanoscience, an international team of physicists with participants from Konstanz has achieved a breakthrough in understanding heat transport

Im Focus: DNA repair: a new letter in the cell alphabet

Results reveal how discoveries may be hidden in scientific “blind spots”

Cells need to repair damaged DNA in our genes to prevent the development of cancer and other diseases. Our cells therefore activate and send “repair-proteins”...

Im Focus: Dresdner scientists print tomorrow’s world

The Fraunhofer IWS Dresden and Technische Universität Dresden inaugurated their jointly operated Center for Additive Manufacturing Dresden (AMCD) with a festive ceremony on February 7, 2017. Scientists from various disciplines perform research on materials, additive manufacturing processes and innovative technologies, which build up components in a layer by layer process. This technology opens up new horizons for component design and combinations of functions. For example during fabrication, electrical conductors and sensors are already able to be additively manufactured into components. They provide information about stress conditions of a product during operation.

The 3D-printing technology, or additive manufacturing as it is often called, has long made the step out of scientific research laboratories into industrial...

Im Focus: Mimicking nature's cellular architectures via 3-D printing

Research offers new level of control over the structure of 3-D printed materials

Nature does amazing things with limited design materials. Grass, for example, can support its own weight, resist strong wind loads, and recover after being...

Im Focus: Three Magnetic States for Each Hole

Nanometer-scale magnetic perforated grids could create new possibilities for computing. Together with international colleagues, scientists from the Helmholtz Zentrum Dresden-Rossendorf (HZDR) have shown how a cobalt grid can be reliably programmed at room temperature. In addition they discovered that for every hole ("antidot") three magnetic states can be configured. The results have been published in the journal "Scientific Reports".

Physicist Dr. Rantej Bali from the HZDR, together with scientists from Singapore and Australia, designed a special grid structure in a thin layer of cobalt in...

All Focus news of the innovation-report >>>

Anzeige

Anzeige

Event News

Booth and panel discussion – The Lindau Nobel Laureate Meetings at the AAAS 2017 Annual Meeting

13.02.2017 | Event News

Complex Loading versus Hidden Reserves

10.02.2017 | Event News

International Conference on Crystal Growth in Freiburg

09.02.2017 | Event News

 
Latest News

Biocompatible 3-D tracking system has potential to improve robot-assisted surgery

17.02.2017 | Medical Engineering

Real-time MRI analysis powered by supercomputers

17.02.2017 | Medical Engineering

Antibiotic effective against drug-resistant bacteria in pediatric skin infections

17.02.2017 | Health and Medicine

VideoLinks
B2B-VideoLinks
More VideoLinks >>>