Forum for Science, Industry and Business

Sponsored by:     3M 
Search our Site:

 

Prions and retroviruses – an unholy alliance?

07.09.2007
Expression of endogenous retroviruses is changed after prion infection

In work originating from the Bavarian Research Cooperation Prions (FORPRION), which ended in 2007, a team led by the scientist Prof. Dr. Christine Leib-Mösch has been able to show that prion proteins may activate endogenous retroviruses in infected brain cells.

In the Institute of Molecular Virology of the GSF – National Research Center for Environment and Health in Neuherberg/Munich (Helmholtz Association of German Research Centres) the group is continuing to search for cellular components whose make-up is changed as a result of a prion infection. In collaboration with colleagues from the Technical University of Munich and the University of Heidelberg, the group used micro-array technologies – micro-arrays are chips with thousands or tens of thousands of DNA or protein probes - and could demonstrate that the expression of endogenous retroviruses is influenced by infectious prion proteins in tests with mouse cells.

Prions – an abbreviation for proteinaceous infectious particles – work as a trigger to a set of diseases of the brain and nervous system, the so-called spongiform encephalopathies. These include BSE in cattle, scrapie in sheep and Creutzfeldt Jakob’s Disease in humans. Prions are structural variants of a normal protein found in healthy tissues – especially in the brain. The devastating effect of infectious prions is that, once they have entered the organism, they can modify the normal "healthy" prion proteins to create more infectious prions, and thus cause the illness to progress. However, as yet, little is known about the molecular mechanisms of pathogenesis, the role of co-factors and the interaction of prion proteins with cellular components.

Retroviruses insert their genetic information into the genome of host cells. In the case of endogenous retroviruses, this involves retroviral infections from long ago, which were transmitted through many generations by means of the germ line. Nearly ten percent of the genome of mice and humans consists of endogenous retroviral sequences that have accumulated during the course of evolution. Indeed, most structural genes of endogenous retroviruses are inactive, but many regulatory elements, such as binding sites for transcription factors, often remain active and can influence neighbouring cellular genes.

The GSF scientists infected mouse neural cells kept in culture with infectious prion proteins and subsequently analysed the expression patterns of endogenous retroviruses. The results showed that the expression of a set of endogenous retroviral sequences is influenced by the prion infection: in comparison with uninfected cells, the expression partly increased but also partly decreased – depending on the cell line and the type of endogenous retroviruses. These effects could be suppressed by pentosan-polysulphate, an anti-prion drug, which means that the influence of the expression can be attributed to the prions and not to some secondary effects.

These observations suggest that prion proteins may stimulate the production of retroviral particles by activation of endogenous retroviruses. Subsequently, these retrovirus-like particles could transport prion proteins from cell to cell, and thus spread the infection.

These studies were carried out within the scope of the “Bavarian Research Cooperation Prions” (FORPRION) in the Association of Bavarian Research Cooperations. FORPRION was founded in 2001 following the appearance of the first BSE cases in Bavaria and was financed equally from the budgets of the Bavarian State Ministry for Science, Research and Art, and the Bavarian State Ministry of Health Food and Consumer Affairs.

Through basic and applied research the consortium aims to make progress in the diagnosis and therapy of human and animal prion diseases, as well as in the field of preventive consumer protection. FORPRION linked up 25 projects, based at five Bavarian universities and in institutes of the Max Planck Society. The financial support of the Bavarian Research Cooperation Prions FORPRION ended in June, 2007.

Michael van den Heuvel | alfa
Further information:
http://www.gsf.de/neu/Aktuelles/Presse/2007/prionen_en.php

Further reports about: Cooperation FORPRION Infection endogenous prion protein prions retroviral retroviruses

More articles from Life Sciences:

nachricht How cheetahs stay fit and healthy
24.03.2017 | Forschungsverbund Berlin e.V.

nachricht When Air is in Short Supply - Shedding light on plant stress reactions when oxygen runs short
23.03.2017 | Institut für Pflanzenbiochemie

All articles from Life Sciences >>>

The most recent press releases about innovation >>>

Die letzten 5 Focus-News des innovations-reports im Überblick:

Im Focus: Giant Magnetic Fields in the Universe

Astronomers from Bonn and Tautenburg in Thuringia (Germany) used the 100-m radio telescope at Effelsberg to observe several galaxy clusters. At the edges of these large accumulations of dark matter, stellar systems (galaxies), hot gas, and charged particles, they found magnetic fields that are exceptionally ordered over distances of many million light years. This makes them the most extended magnetic fields in the universe known so far.

The results will be published on March 22 in the journal „Astronomy & Astrophysics“.

Galaxy clusters are the largest gravitationally bound structures in the universe. With a typical extent of about 10 million light years, i.e. 100 times the...

Im Focus: Tracing down linear ubiquitination

Researchers at the Goethe University Frankfurt, together with partners from the University of Tübingen in Germany and Queen Mary University as well as Francis Crick Institute from London (UK) have developed a novel technology to decipher the secret ubiquitin code.

Ubiquitin is a small protein that can be linked to other cellular proteins, thereby controlling and modulating their functions. The attachment occurs in many...

Im Focus: Perovskite edges can be tuned for optoelectronic performance

Layered 2D material improves efficiency for solar cells and LEDs

In the eternal search for next generation high-efficiency solar cells and LEDs, scientists at Los Alamos National Laboratory and their partners are creating...

Im Focus: Polymer-coated silicon nanosheets as alternative to graphene: A perfect team for nanoelectronics

Silicon nanosheets are thin, two-dimensional layers with exceptional optoelectronic properties very similar to those of graphene. Albeit, the nanosheets are less stable. Now researchers at the Technical University of Munich (TUM) have, for the first time ever, produced a composite material combining silicon nanosheets and a polymer that is both UV-resistant and easy to process. This brings the scientists a significant step closer to industrial applications like flexible displays and photosensors.

Silicon nanosheets are thin, two-dimensional layers with exceptional optoelectronic properties very similar to those of graphene. Albeit, the nanosheets are...

Im Focus: Researchers Imitate Molecular Crowding in Cells

Enzymes behave differently in a test tube compared with the molecular scrum of a living cell. Chemists from the University of Basel have now been able to simulate these confined natural conditions in artificial vesicles for the first time. As reported in the academic journal Small, the results are offering better insight into the development of nanoreactors and artificial organelles.

Enzymes behave differently in a test tube compared with the molecular scrum of a living cell. Chemists from the University of Basel have now been able to...

All Focus news of the innovation-report >>>

Anzeige

Anzeige

Event News

International Land Use Symposium ILUS 2017: Call for Abstracts and Registration open

20.03.2017 | Event News

CONNECT 2017: International congress on connective tissue

14.03.2017 | Event News

ICTM Conference: Turbine Construction between Big Data and Additive Manufacturing

07.03.2017 | Event News

 
Latest News

NASA examines Peru's deadly rainfall

24.03.2017 | Earth Sciences

What does congenital Zika syndrome look like?

24.03.2017 | Health and Medicine

Steep rise of the Bernese Alps

24.03.2017 | Earth Sciences

VideoLinks
B2B-VideoLinks
More VideoLinks >>>