Forum for Science, Industry and Business

Sponsored by:     3M 
Search our Site:

 

A low expression of MX2 gene exists in the white blood cells of narcoleptics

02.08.2007
The first report to identify the biological markers of narcolepsy using gene expression in white blood cells finds that the MX2 gene, which is relevant to the immune system, is significantly less expressed in narcoleptics compared with normal subjects. This underlies the abnormalities in the blood cells of persons suffering from narcolepsy, according to a study published in the August 1st issue of the journal SLEEP.

Susumu Tanaka, PhD, of the Tokyo Institute of Psychiatry in Japan, conducted the study from a pool of total ribonucleic acid (RNA) – a nucleic acid polymer that plays several important roles in the processes that translate genetic information from DNA into protein products – from 12 patients with narcolepsy with cataplexy and from 12 age- and sex-matched healthy controls. The pooled samples were initially screened for candidate genes for narcolepsy by differential display analysis using annealing control primers (ACP).

The second screening of the samples was carried out by semiquantitative polymerase chain reaction (PCR) – a biochemistry and molecular biology technique for isolating and exponentially amplifying a fragment or sequence of interest of DNA, via enzymatic replication, without using a living organism – using gene-specific primers.

Finally, the expression levels of the candidate genes were further confirmed by quantitative real-time PCR using a new set of samples: 20 patients with narcolepsy with cataplexy and 20 healthy controls.

... more about:
»MX2 »White »disorder »narcolepsy »narcoleptic »sleep

According to the study, the second screening revealed differential expression of four candidate genes, among which MX2 was confirmed as a significantly down-regulated gene in the white blood cells of narcoleptic patients by quantitative real-time PCR.

“In narcolepsy, it has been suggested that specific alterations in the immune system occur, and it’s important for pathophysiology of this disorder,” said Tanaka. “We thought that we can get these specific alterations by a differential display method using white blood cells. In this study, we applied the new ACP technology to RNA extracted from blood cells and successfully identified the MX2 gene as a dysregulated gene in narcoleptic patients. However, the direct relationship of this gene in narcolepsy has not been elucidated. Further study is needed to explore the functional relationship between the MX2 gene and narcolepsy and characterize the effect of interferons in narcolepsy.”

Narcolepsy is a sleep disorder that causes people to fall asleep uncontrollably during the day. It also includes features of dreaming that occur while awake. Other common symptoms include sleep paralysis, hallucinations and cataplexy.

About one out of every 2,000 people is known to have narcolepsy. The chance that you have narcolepsy is higher when a relative also has it. It is very rare for more than two people in the same family to have this sleep disorder. It affects the same number of men and women.

Those who suspect they might be suffering from narcolepsy, or another sleep disorder, are urged to consult with their primary care doctor or a sleep specialist.

Jim Arcuri | EurekAlert!
Further information:
http://www.aasmnet.org

Further reports about: MX2 White disorder narcolepsy narcoleptic sleep

More articles from Life Sciences:

nachricht 'Y' a protein unicorn might matter in glaucoma
23.10.2017 | Georgia Institute of Technology

nachricht Microfluidics probe 'cholesterol' of the oil industry
23.10.2017 | Rice University

All articles from Life Sciences >>>

The most recent press releases about innovation >>>

Die letzten 5 Focus-News des innovations-reports im Überblick:

Im Focus: Salmonella as a tumour medication

HZI researchers developed a bacterial strain that can be used in cancer therapy

Salmonellae are dangerous pathogens that enter the body via contaminated food and can cause severe infections. But these bacteria are also known to target...

Im Focus: Neutron star merger directly observed for the first time

University of Maryland researchers contribute to historic detection of gravitational waves and light created by event

On August 17, 2017, at 12:41:04 UTC, scientists made the first direct observation of a merger between two neutron stars--the dense, collapsed cores that remain...

Im Focus: Breaking: the first light from two neutron stars merging

Seven new papers describe the first-ever detection of light from a gravitational wave source. The event, caused by two neutron stars colliding and merging together, was dubbed GW170817 because it sent ripples through space-time that reached Earth on 2017 August 17. Around the world, hundreds of excited astronomers mobilized quickly and were able to observe the event using numerous telescopes, providing a wealth of new data.

Previous detections of gravitational waves have all involved the merger of two black holes, a feat that won the 2017 Nobel Prize in Physics earlier this month....

Im Focus: Smart sensors for efficient processes

Material defects in end products can quickly result in failures in many areas of industry, and have a massive impact on the safe use of their products. This is why, in the field of quality assurance, intelligent, nondestructive sensor systems play a key role. They allow testing components and parts in a rapid and cost-efficient manner without destroying the actual product or changing its surface. Experts from the Fraunhofer IZFP in Saarbrücken will be presenting two exhibits at the Blechexpo in Stuttgart from 7–10 November 2017 that allow fast, reliable, and automated characterization of materials and detection of defects (Hall 5, Booth 5306).

When quality testing uses time-consuming destructive test methods, it can result in enormous costs due to damaging or destroying the products. And given that...

Im Focus: Cold molecules on collision course

Using a new cooling technique MPQ scientists succeed at observing collisions in a dense beam of cold and slow dipolar molecules.

How do chemical reactions proceed at extremely low temperatures? The answer requires the investigation of molecular samples that are cold, dense, and slow at...

All Focus news of the innovation-report >>>

Anzeige

Anzeige

Event News

3rd Symposium on Driving Simulation

23.10.2017 | Event News

ASEAN Member States discuss the future role of renewable energy

17.10.2017 | Event News

World Health Summit 2017: International experts set the course for the future of Global Health

10.10.2017 | Event News

 
Latest News

Microfluidics probe 'cholesterol' of the oil industry

23.10.2017 | Life Sciences

Gamma rays will reach beyond the limits of light

23.10.2017 | Physics and Astronomy

The end of pneumonia? New vaccine offers hope

23.10.2017 | Health and Medicine

VideoLinks
B2B-VideoLinks
More VideoLinks >>>