Forum for Science, Industry and Business

Sponsored by:     3M 
Search our Site:

 

3 proteins may play important role in nerve-cell repair

13.04.2007
Some mature brain cells can grow new extensions when the amount of three particular proteins on their surface increases, a new study shows.

The research examined three related receptor proteins, called GPR3, GPR6 and GPR12, on nerve cells in the brains of rats.

When researchers increased the amount of the three proteins, the cells grew extensions that were up to three times longer than those on nerve cells with normal levels of the proteins, and the extensions grew four to eight times faster than in control cells.

"Our findings suggest that these three proteins could be important targets for treating stroke, brain and spinal cord injuries and also neurodegenerative diseases," says principal investigator Yoshinaga Saeki of the Ohio State University Medical Center.

... more about:
»Axon »GPR12 »GPR3 »cAMP »extensions »nerve cells

The study is published in the April 6 issue of the Journal of Biological Chemistry.

Increased amounts of the proteins were associated with a significant rise in the level of an important signaling molecule inside the nerve cells called cAMP. This molecule plays a key role in regulating nerve-cell growth, differentiation and survival, and the regeneration of long parts of the cell called axons that carry the nerve impulses.

Levels of cAMP drop in mammalian nerve cells as they mature, and this is thought to explain, in part, why mature nerve cells cannot regenerate damaged axons.

"Our findings provide additional evidence that cAMP plays an important role in axon growth and suggest that these receptors are likely to play a major role in regulating cAMP production in nerve cells," says Saeki, an associate professor of neurological surgery and chief of Ohio State's Dardinger Laboratory for Neuro-oncology and Neurosciences.

In this study, first author Shigeru Tanaka, a postdoctoral fellow in Saeki's laboratory, and his colleagues used nerve cells obtained from the brain tissue of rats and mouse neuroblastoma cells growing in culture to learn more about the three proteins and their involvement in cAMP regulation.

The researchers added additional copies of the three genes to the cells to increase the levels of the proteins, and then used a laboratory technique called RNA intereference to turn off production of the proteins.

Of the three molecules, GPR3 was the most abundant in the nerve cells, while GPR12 was the most potent at stimulating growth of the nerve extensions. The study showed that blocking GPR3 greatly slows the growth of the nerve extensions. The researchers reversed this effect by restoring either GPR3 or GPR12 in the cells.

High levels of the three proteins were also linked to higher levels of cAMP, with GPR6 and GPR12 increasing the level twofold to threefold.

"Taken together," Saeki says, "our findings indicate that these three proteins improve growth of neuronal extensions even in the presence of inhibitory molecules, and we are very keen to find out whether the approach can be translated in preclinical animal models of stroke or spinal cord injury."

Darrell E. Ward | EurekAlert!
Further information:
http://www.osumc.edu

Further reports about: Axon GPR12 GPR3 cAMP extensions nerve cells

More articles from Life Sciences:

nachricht Meadows beat out shrubs when it comes to storing carbon
23.11.2017 | Norwegian University of Science and Technology

nachricht Migrating Cells: Folds in the cell membrane supply material for necessary blebs
23.11.2017 | Westfälische Wilhelms-Universität Münster

All articles from Life Sciences >>>

The most recent press releases about innovation >>>

Die letzten 5 Focus-News des innovations-reports im Überblick:

Im Focus: New proton record: Researchers measure magnetic moment with greatest possible precision

High-precision measurement of the g-factor eleven times more precise than before / Results indicate a strong similarity between protons and antiprotons

The magnetic moment of an individual proton is inconceivably small, but can still be quantified. The basis for undertaking this measurement was laid over ten...

Im Focus: Frictional Heat Powers Hydrothermal Activity on Enceladus

Computer simulation shows how the icy moon heats water in a porous rock core

Heat from the friction of rocks caused by tidal forces could be the “engine” for the hydrothermal activity on Saturn's moon Enceladus. This presupposes that...

Im Focus: Nanoparticles help with malaria diagnosis – new rapid test in development

The WHO reports an estimated 429,000 malaria deaths each year. The disease mostly affects tropical and subtropical regions and in particular the African continent. The Fraunhofer Institute for Silicate Research ISC teamed up with the Fraunhofer Institute for Molecular Biology and Applied Ecology IME and the Institute of Tropical Medicine at the University of Tübingen for a new test method to detect malaria parasites in blood. The idea of the research project “NanoFRET” is to develop a highly sensitive and reliable rapid diagnostic test so that patient treatment can begin as early as possible.

Malaria is caused by parasites transmitted by mosquito bite. The most dangerous form of malaria is malaria tropica. Left untreated, it is fatal in most cases....

Im Focus: A “cosmic snake” reveals the structure of remote galaxies

The formation of stars in distant galaxies is still largely unexplored. For the first time, astron-omers at the University of Geneva have now been able to closely observe a star system six billion light-years away. In doing so, they are confirming earlier simulations made by the University of Zurich. One special effect is made possible by the multiple reflections of images that run through the cosmos like a snake.

Today, astronomers have a pretty accurate idea of how stars were formed in the recent cosmic past. But do these laws also apply to older galaxies? For around a...

Im Focus: Visual intelligence is not the same as IQ

Just because someone is smart and well-motivated doesn't mean he or she can learn the visual skills needed to excel at tasks like matching fingerprints, interpreting medical X-rays, keeping track of aircraft on radar displays or forensic face matching.

That is the implication of a new study which shows for the first time that there is a broad range of differences in people's visual ability and that these...

All Focus news of the innovation-report >>>

Anzeige

Anzeige

Event News

Ecology Across Borders: International conference brings together 1,500 ecologists

15.11.2017 | Event News

Road into laboratory: Users discuss biaxial fatigue-testing for car and truck wheel

15.11.2017 | Event News

#Berlin5GWeek: The right network for Industry 4.0

30.10.2017 | Event News

 
Latest News

Heidelberg Researchers Study Unique Underwater Stalactites

24.11.2017 | Earth Sciences

Species may appear deceptively resilient to climate change

24.11.2017 | Ecology, The Environment and Conservation

Lightning, with a chance of antimatter

24.11.2017 | Earth Sciences

VideoLinks
B2B-VideoLinks
More VideoLinks >>>