The research is a collaboration between molecular biologists and physicists. ”The molecular biologists have knowledge of how the cell functions and of the interplay between the intercellular parts, while the physicists have the expertise and the technique to be able to measure and analyze the physical processes.” says Lene Oddershede, physicist at the Niels Bohr Institute, University of Copenhagen. This interdisciplinary work between physics and biology has been very fruitful and will be published April 3rd in the prestigious scientific journal PNAS, Proceedings of the National Academy of Sciences.
The researchers have investigated how a virus exploits the machinery of human cells to produce the proteins which the virus needs in order to replicate to billions of new vira. The virus penetrates into the host cell where it liberates its RNA which is a copy of the heritage material, DNA. RNA is like a 'cook book' which contains the recipes of which proteins the virus needs for replication.
The work process of a virus
The cell has ribosomes, a kind of 'molecular motors', which move along the RNA and read the code for the proteins to be produced to fulfill the needs of the living cell. The task of the ribosomes is to read the code of the host cell, but the virus has the special trick that its RNA resembles that of the host cell, and hence, the ribosomes of the host cell will start reading the viral RNA and produce the proteins requested by the virus. In order words, the virus can be viewed as a parasite, exploiting the human cell to live and replicate in.
Viral RNA resembles human RNA, but it has a tendency to curl up into 'pseudoknots', a three dimensional structure. When the ribosome walking along an RNA encounters a pseudoknot it needs to unravel the pseudoknot before it can proceed. Question is, how does it do that? Lene Oddershede at the Niels Bohr Institute, University of Copenhagen has developed optical tweezers which can investigate and manipulate molecules at the nano-meter scale. Using a tightly focussed laserbeam this instrument can grab the ends of the RNA tether and follow the process of how the pseudoknot is mechanically unfolded.
A crucial slip of the cellular motor
In their investigations the researchers use a pseudoknot which is related to bird flu. When the ribosome encounters a pseudoknot it has to unravel the knot before the reading can proceed. During this process the ribosome sometimes slips backwards and, like the letters making up a word, it now reads a new RNA sequence and hence uses another recipe to construct the protein. The researchers have found that the stronger the pseudoknot the more often this backwards slipping happens. The different protein formed is the protein needed by the virus, with possible serious consequences for the hosting organism. This is the manner in which many vira, e.g. HIV, trick the cell into producing something which it never would have done otherwise. Understanding the role of the pseudoknots can be an important step in developing a viral vaccine.
Gertie Skaarup | EurekAlert!
Protein 'spy' gains new abilities
28.04.2017 | Rice University
How Plants Form Their Sugar Transport Routes
28.04.2017 | Universität Heidelberg
More and more automobile companies are focusing on body parts made of carbon fiber reinforced plastics (CFRP). However, manufacturing and repair costs must be further reduced in order to make CFRP more economical in use. Together with the Volkswagen AG and five other partners in the project HolQueSt 3D, the Laser Zentrum Hannover e.V. (LZH) has developed laser processes for the automatic trimming, drilling and repair of three-dimensional components.
Automated manufacturing processes are the basis for ultimately establishing the series production of CFRP components. In the project HolQueSt 3D, the LZH has...
Reflecting the structure of composites found in nature and the ancient world, researchers at the University of Illinois at Urbana-Champaign have synthesized thin carbon nanotube (CNT) textiles that exhibit both high electrical conductivity and a level of toughness that is about fifty times higher than copper films, currently used in electronics.
"The structural robustness of thin metal films has significant importance for the reliable operation of smart skin and flexible electronics including...
The nearby, giant radio galaxy M87 hosts a supermassive black hole (BH) and is well-known for its bright jet dominating the spectrum over ten orders of magnitude in frequency. Due to its proximity, jet prominence, and the large black hole mass, M87 is the best laboratory for investigating the formation, acceleration, and collimation of relativistic jets. A research team led by Silke Britzen from the Max Planck Institute for Radio Astronomy in Bonn, Germany, has found strong indication for turbulent processes connecting the accretion disk and the jet of that galaxy providing insights into the longstanding problem of the origin of astrophysical jets.
Supermassive black holes form some of the most enigmatic phenomena in astrophysics. Their enormous energy output is supposed to be generated by the...
The probability to find a certain number of photons inside a laser pulse usually corresponds to a classical distribution of independent events, the so-called...
Microprocessors based on atomically thin materials hold the promise of the evolution of traditional processors as well as new applications in the field of flexible electronics. Now, a TU Wien research team led by Thomas Müller has made a breakthrough in this field as part of an ongoing research project.
Two-dimensional materials, or 2D materials for short, are extremely versatile, although – or often more precisely because – they are made up of just one or a...
28.04.2017 | Event News
20.04.2017 | Event News
18.04.2017 | Event News
28.04.2017 | Life Sciences
28.04.2017 | Life Sciences
28.04.2017 | Life Sciences