Speed is a primary concern in pneumonic plague, which kills in three to four days and potentially could be used in a terrorist attack. The bacterium that causes plague, Yersinia pestis, is vulnerable to antibiotics, but by the time an unusual infection becomes evident, Yersinia often has gained an unbeatable upper hand.
"By the time most doctors recognize an infection as plague, rather than the flu, it's already too late to begin antibiotic treatment," says senior author William Goldman, Ph.D., professor of molecular microbiology. "That makes pneumonic plague a concern both because of its rare natural outbreaks, one of which began in the Congo in 2005, and because of its potential use as a bioweapon."
Yersinia is best known for causing the Black Death in the Middle Ages in Europe, when historians estimate it killed a third or more of the population. Depending on how Yersinia is introduced, the versatile pathogen can modify itself to infect the lungs (pneumonic plague), the lymph glands (bubonic plague), or the bloodstream and organs (septicemic plague). Bubonic plague was spread by bites from infected fleas; pneumonic plague can spread through droplets of moisture expelled by coughing and sneezing.
With pilot project funding from the Midwest Regional Center for Excellence in Biodefense and Emerging Infectious Diseases Research, Wyndham Lathem, Ph.D., a postdoctoral fellow in Goldman's laboratory, developed a mouse model of pneumonic plague and showed that it had many similarities to human infection. In mice, pneumonic plague causes the lungs to fill up with a fluid composed of bacteria, inflammatory cells and other substances. Shortly before infected mice die, the bacteria also begin showing up in the spleen and other organs, spreading there via the bloodstream.
Previous research had suggested that pneumonic plague might be spreading in the body in part through use of a protein known as plasminogen activator (PLA). The protein is a protease, which degrades other proteins. Goldman, Lathem and colleagues thought PLA might be a tool Yersinia uses to break open protective blood clots that form around pockets of infection. This clotting response is believed to be a way the body attempts to limit the spread of infections: Surround a pathogen with blood clots, and it can't reproduce and spread. Scientists speculated that breaking open the clots might be how Yersinia opened a path from the lungs into the blood.
When scientists infected mice with Yersinia that lacked PLA, though, they found infection ebbing in the lungs but spreading to the spleen. The mice still died, but it took them several days longer to do so. They concluded that the aggressive pneumonia and rapid death of pneumonic plague appears to depend on the activity of PLA.
"Pharmaceutical companies have large libraries of protease inhibitors, so hopefully someone will start the search soon for an inhibitor of PLA that is specific and non-toxic enough to be used as an adjunct treatment," Goldman says. "That might give us enough time to use antibiotics to save patients afflicted with pneumonic plague." Goldman hopes to conduct follow-up studies to learn more about how plague exploits PLA.
Michael C. Purdy | EurekAlert!
Link Discovered between Immune System, Brain Structure and Memory
26.04.2017 | Universität Basel
Researchers develop eco-friendly, 4-in-1 catalyst
25.04.2017 | Brown University
More and more automobile companies are focusing on body parts made of carbon fiber reinforced plastics (CFRP). However, manufacturing and repair costs must be further reduced in order to make CFRP more economical in use. Together with the Volkswagen AG and five other partners in the project HolQueSt 3D, the Laser Zentrum Hannover e.V. (LZH) has developed laser processes for the automatic trimming, drilling and repair of three-dimensional components.
Automated manufacturing processes are the basis for ultimately establishing the series production of CFRP components. In the project HolQueSt 3D, the LZH has...
Reflecting the structure of composites found in nature and the ancient world, researchers at the University of Illinois at Urbana-Champaign have synthesized thin carbon nanotube (CNT) textiles that exhibit both high electrical conductivity and a level of toughness that is about fifty times higher than copper films, currently used in electronics.
"The structural robustness of thin metal films has significant importance for the reliable operation of smart skin and flexible electronics including...
The nearby, giant radio galaxy M87 hosts a supermassive black hole (BH) and is well-known for its bright jet dominating the spectrum over ten orders of magnitude in frequency. Due to its proximity, jet prominence, and the large black hole mass, M87 is the best laboratory for investigating the formation, acceleration, and collimation of relativistic jets. A research team led by Silke Britzen from the Max Planck Institute for Radio Astronomy in Bonn, Germany, has found strong indication for turbulent processes connecting the accretion disk and the jet of that galaxy providing insights into the longstanding problem of the origin of astrophysical jets.
Supermassive black holes form some of the most enigmatic phenomena in astrophysics. Their enormous energy output is supposed to be generated by the...
The probability to find a certain number of photons inside a laser pulse usually corresponds to a classical distribution of independent events, the so-called...
Microprocessors based on atomically thin materials hold the promise of the evolution of traditional processors as well as new applications in the field of flexible electronics. Now, a TU Wien research team led by Thomas Müller has made a breakthrough in this field as part of an ongoing research project.
Two-dimensional materials, or 2D materials for short, are extremely versatile, although – or often more precisely because – they are made up of just one or a...
20.04.2017 | Event News
18.04.2017 | Event News
03.04.2017 | Event News
26.04.2017 | Materials Sciences
26.04.2017 | Agricultural and Forestry Science
26.04.2017 | Physics and Astronomy