Scientists also create modified plants to identify and characterize the functions of specific genes. The current issue of Cold Spring Harbor Protocols—released online today (www.cshprotocols.org)—includes a set of techniques for the creation of transgenic plants.
One of the protocols, freely available at http://www.cshprotocols.org/cgi/content/full/2006/30/pdb.prot4668, describes the use of a bacterium, Agrobacterium, to create transgenic Arabidopsis plants. Arabidopsis is used in many studies due to its short reproductive cycle, ease of cultivation, and close relatedness to economically important species such as broccoli and cauliflower. Agrobacterium contains a small chromosome—called the Ti plasmid—into which scientists can insert a gene of interest.
This ‘transgene’ is transferred to Arabidopsis through natural infection with Agrobacterium.
The highlighted article from CSH Protocols describes three techniques that encourage Agrobacterium to infect Arabidopsis plants: dipping an Arabidopsis flower directly into a solution containing Agrobacterium, mechanically forcing the Agrobacterium into the plant cells by applying vacuum, and simply spraying an Agrobacterium suspension onto the plants.
Cells communicate in a dynamic code
19.02.2018 | California Institute of Technology
Studying mitosis' structure to understand the inside of cancer cells
19.02.2018 | Biophysical Society
For the first time, a team of researchers at the Max-Planck Institute (MPI) for Polymer Research in Mainz, Germany, has succeeded in making an integrated circuit (IC) from just a monolayer of a semiconducting polymer via a bottom-up, self-assembly approach.
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Breakthrough provides a new concept of the design of molecular motors, sensors and electricity generators at nanoscale
Researchers from the Institute of Organic Chemistry and Biochemistry of the CAS (IOCB Prague), Institute of Physics of the CAS (IP CAS) and Palacký University...
For photographers and scientists, lenses are lifesavers. They reflect and refract light, making possible the imaging systems that drive discovery through the microscope and preserve history through cameras.
But today's glass-based lenses are bulky and resist miniaturization. Next-generation technologies, such as ultrathin cameras or tiny microscopes, require...
Scientists from the University of Zurich have succeeded for the first time in tracking individual stem cells and their neuronal progeny over months within the intact adult brain. This study sheds light on how new neurons are produced throughout life.
The generation of new nerve cells was once thought to taper off at the end of embryonic development. However, recent research has shown that the adult brain...
Theoretical physicists propose to use negative interference to control heat flow in quantum devices. Study published in Physical Review Letters
Quantum computer parts are sensitive and need to be cooled to very low temperatures. Their tiny size makes them particularly susceptible to a temperature...
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