In this respect, E. coli is a model bacterium to study the continuum between commensal and pathogenic bacteria. Researchers at INRA in Toulouse, in collaboration with German universities in Würzburg and Göttingen and the Institut Pasteur in Paris, have shown for the first time that both commensal and pathogenic E. coli produce a substance which is toxic to the DNA in eukaryotic cells. The bacteria producing this toxin thus induce DNA breaks in host cells and disturb the cell cycle. This slowdown of eukaryotic cell proliferation may enhance bacterial colonization of the intestine. On the other hand, if these breaks are not repaired, they could give rise to a high level of mutations, which are the principal factors triggering cancer in man. The details of this work have been published in Science, August 11th 2006.
Colibactin, a new toxin which affects the host cell cycle
Certain strains of E. coli produce a toxin, which induces a toxic effect in host cells, characterised by gradual cell enlargement following the arrest of cell proliferation. INRA researchers in Toulouse, in collaboration with teams at the German universities of Würzburg and Göttingen and the Institut Pasteur in Paris, have demonstrated that these bacterial strains possess a "genomic island" in their genome, which contains all genes allowing the biosynthesis of a new toxin, which they have called "Colibactin". The researchers have shown that the bacteria producing this toxin induce serious lesions to the DNA of host cells, causing a blockade of the cell cycle of infected cells. Colibactin belongs to a new family of bacterial toxins, which are able to act on the cell cycle of eukaryotic cells. The INRA researchers have proposed to call this family the "cyclomodulins".
Colibactin is a non-protein toxin. The genes carried by the genomic island code for several enzymes belonging to the family of "polyketide synthetases" (PKS) and "nonribosomal polypeptide synthetases" (NRPS). Compounds arising from these biosynthetic pathways constitute a large family of natural products with a very broad range of biological activities and pharmacological properties. This family comprises numerous molecules which are of importance both agronomically (anti-parasite substances, such as avermectin) and medically (e.g. immunosuppressants, cholesterol-lowering agents, anticancer compounds and antibiotics (cyclosporine, lovastatin, bleomycin, erythromycin, etc.). This is the first time that an enzyme system of this type, producing a molecule active on eukaryote cells, has been characterised in E. coli, a bacterial species where genetic engineering is well mastered. This discovery provides a biotechnological key to producing new compounds of interest, and has been the subject of a patent application. It opens the way to novel therapeutic approaches as well as preventive opportunities.
Infectious diseases, cancer and anti-proliferative effects: is there a role for bacteria producing cyclomodulins?
The work reported in Science also raises an important question for public health. DNA double strand breaks are dangerous lesions affecting eukaryotic cells; if these are not repaired, they give rise to a high level of mutations, which are the principal triggers of cancer in man. Colibactin is produced by both commensal E. coli in the intestinal flora and pathogenic strains which are responsible for septicaemia, urinary tract infections and meningitis. The presence of these bacteria in the commensal flora may therefore constitute a predisposing factor for the development of certain cancers. Thus bacterial flora may participate in the development, differentiation and homeostasis of mucosa and hence the development of certain types of cancer, or protection against them.
Céline Goupil | alfa
Novel mechanisms of action discovered for the skin cancer medication Imiquimod
21.10.2016 | Technische Universität München
Second research flight into zero gravity
21.10.2016 | Universität Zürich
Researchers from the Institute for Quantum Computing (IQC) at the University of Waterloo led the development of a new extensible wiring technique capable of controlling superconducting quantum bits, representing a significant step towards to the realization of a scalable quantum computer.
"The quantum socket is a wiring method that uses three-dimensional wires based on spring-loaded pins to address individual qubits," said Jeremy Béjanin, a PhD...
In a paper in Scientific Reports, a research team at Worcester Polytechnic Institute describes a novel light-activated phenomenon that could become the basis for applications as diverse as microscopic robotic grippers and more efficient solar cells.
A research team at Worcester Polytechnic Institute (WPI) has developed a revolutionary, light-activated semiconductor nanocomposite material that can be used...
By forcefully embedding two silicon atoms in a diamond matrix, Sandia researchers have demonstrated for the first time on a single chip all the components needed to create a quantum bridge to link quantum computers together.
"People have already built small quantum computers," says Sandia researcher Ryan Camacho. "Maybe the first useful one won't be a single giant quantum computer...
COMPAMED has become the leading international marketplace for suppliers of medical manufacturing. The trade fair, which takes place every November and is co-located to MEDICA in Dusseldorf, has been steadily growing over the past years and shows that medical technology remains a rapidly growing market.
In 2016, the joint pavilion by the IVAM Microtechnology Network, the Product Market “High-tech for Medical Devices”, will be located in Hall 8a again and will...
'Ferroelectric' materials can switch between different states of electrical polarization in response to an external electric field. This flexibility means they show promise for many applications, for example in electronic devices and computer memory. Current ferroelectric materials are highly valued for their thermal and chemical stability and rapid electro-mechanical responses, but creating a material that is scalable down to the tiny sizes needed for technologies like silicon-based semiconductors (Si-based CMOS) has proven challenging.
Now, Hiroshi Funakubo and co-workers at the Tokyo Institute of Technology, in collaboration with researchers across Japan, have conducted experiments to...
14.10.2016 | Event News
14.10.2016 | Event News
12.10.2016 | Event News
21.10.2016 | Health and Medicine
21.10.2016 | Information Technology
21.10.2016 | Materials Sciences