Forum for Science, Industry and Business

Sponsored by:     3M 
Search our Site:

 

Scientists Create a New Way to Study T Cell Signaling

18.11.2005


An experiment that began as a “fantasy pipe dream” just three years ago is now a reality. Researchers with the Lawrence Berkeley National Laboratory (Berkeley Lab) and the University of California at Berkeley, combining nanotechnology with biochemistry, have created unique synthetic membranes that, for the first time ever, enable them to directly control signaling activity in living T cells from the immune system. Already their experiments have yielded surprising results.


These fluorescently labeled electron micrographs show immunological synapses formed by T cell receptors (green) and adhesion molecules (red). Image (A) shows the synapse in its natural bull’s eye shape; in image (B) chromium lines were used to pattern the synapse with parallel lines; (C) the synapse was patterned into a square grid; and (D), the synapse was patterned into concentric hexagons.


This watercolor painting by Raghuveer Parthasarathy, a member of Jay Groves research group, shows a hybrid interface between a living T cell and a synthetic membrane on a substrate that has been patterned with chromium lines. T cell receptors (TCRs) are communicating with their corresponding signaling ligands on the membrane. By controlling the spatial arrangements of the signaling ligands, scientists can control the T cell’s overall response.



“This marriage of inorganic nanotechnology with organic molecules and cells enables us to go inside a living cell and physically move around its signaling molecules with molecular precision,” said Jay Groves, a chemist who holds a joint appointment with Berkeley Lab’s Physical Biosciences Division and UC Berkeley’s Chemistry Department. “Our experimental beaker has now become the inside of living cells and we can watch chemical reactions take place there.”

Groves is the principal co-author, along with Michael Dustin, a cellular immunologist at New York University (NYU), of a paper published in the November 18, 2005 issue of the journal Science, entitled: “Altered TCR Signaling from Geometrically Repatterned Immunological Synapses.” The lead author is Kaspar Mossman, a graduate student in Groves’ research group, and the second co-author is Gabriele Campi, a graduate student at NYU with Dustin.


“Scientists, including ourselves, have been posing elaborate theories about how the strength and duration of signals that activate T cells are controlled by immunological synapses, without having been able to do direct experimentation of key factors,” said Groves. “Three years ago, we had this fantasy pipe dream about an experiment to measure how alterations in the geometric shapes of the synapses – what we call spatial mutations – would affect T cell signaling. Then we realized, we have the tools to create nanoscale patterns, we can do this.”

The human immune system is a remarkable collaboration of different types of cells, working together to protect our bodies from bacterial, parasitic, fungal or viral infections, and against the growth of tumors. The process starts when “antigens,” special markers on the surface of a cell, identify another cell as “non-self,” and signal the cellular warriors of the immune system to kill the invader. Leading this attack will be the T cells, lymphocytes from the thymus. It is well established that the key to T cell activation is the molecular signal coming off antigen-presenting cell surfaces. This signal must be enhanced and sustained long enough for the T cells to commit to mounting an immune response, and then must be cut off in time to avoid antigen-induced cell suicide or “apoptosis” of the T cells.

It has also been established that the control center for T cell signaling is at the junction or point of contact between T cells and antigens, dubbed the “immunological synapse” because it resembles the synapse between two communicating nerve cells. At the immunological synapse, a central cluster of T cell receptors surrounded by a ring of adhesion molecules form what co-author Dustin has described as a sort of “bull’s-eye.” The center of this bull’s eye has been dubbed the “central supramolecular activation cluster,” or c-SMAC, because it was believed to be the source of T cell activation.

“The original idea behind the c-SMAC was that the larger the T cell receptor cluster, the stronger the T cell activation signal,” said Groves. “This simple vision of strength in numbers had begun to show cracks, and now we have demonstrated that just the opposite is true, the coalescence of the c-SMAC cluster extinguishes the T cell activation signal. The duration of the activation signal is related to the spatial organization of the T cell receptors rather than cluster size.”

Groves and his colleagues constructed their synthetic membranes out of lipids which they assembled onto a substrate of solid silica so that the membranes were able to float freely a few nanometers above the substrate. This enabled the researchers to preserve the membranes in their naturally fluid state, allowing lipids and T cell receptor proteins to diffuse and interact freely over macroscopic distances.

“The fluidity of our membranes created artificial antigen-presenting cell surfaces that enabled the formation of functional immunological synapses with living T cells,” said Groves.

Groves and his colleagues were able to spatially mutate the geometric shapes of the immunological synapses by embedding the silica substrate with chrome lines that were only 100 nanometers (about one ten-millionth of an inch) wide. These ultra-narrow chrome lines served as barriers that restricted the motion of membrane lipids and T cell receptor proteins. Using electron-beam lithography, the researchers were able to configure the chrome lines into several distinct patterns, including simple parallel lines, grids, and a series of concentric hexagons.

“By changing the shape of the immunological synapse, we showed that the synapse signal starts out in an amplified mode, and that the transport of the T cell receptors towards the center weakens and eventually extinguishes the signal, irrespective of the degree of clustering,” Groves said. “This may help explain why diseases of the autoimmune system are so difficult to treat. T cell receptor proteins do not respond like a conventional target, where if you hit the bull’s eye you trigger a signal. The spatial position of the receptor determines the type of signal it triggers.”

If scientists can learn more about the impact that spatial arrangement has on the immunological synapse and its signaling strength, the information could benefit the future development of drugs for treating autoimmune diseases. Such information should also help scientists better understand the chemical language by which cells communicate with one another.

Groves said this new technique for spatial mutation studies should be applicable to many intercellular signaling systems. Already, he and his colleagues have begun applying it to study neuronal synapse formation, and cell signaling mechanisms in the development of cancer. They are also using it to look at the dynamic range of signaling over which T cell receptors can respond.

“Essentially, these experiments amount to using inorganic nanotechnology to physically grab a protein in a living cell and move it to another position in that cell – then watch how the cell responds,” said Groves. “We used it to study the T cell as a paradigm system, but the theme here is much more general. Whereas the spatial position of molecules is rarely thought to play an important role in the outcome of a chemical reaction, with our experimental technique we are seeing that, in living cells, this is not the case. The spatial position encodes information which can be directly translated into altered chemical outcomes.”

The earliest indications that spatial positions could influence T cell signaling and that the synaptic pattern might actually help to extinguish the signal came from the work of Arup Chakraborty, a chemical engineering professor who, at the time, held a joint Berkeley Lab/UC Berkeley appointment and is now with MIT University. Chakraborty is a pioneer in the use of computer simulations, called “experiments in silico,” for studying important problems in cellular immunology. In 2003, his computational models indicated that the immunological synapse is responsible for intense but self-limited T cell signaling.

Berkeley Lab is a U.S. Department of Energy national laboratory located in Berkeley, California. It conducts unclassified scientific research and is managed by the University of California.

Lynn Yarris | EurekAlert!
Further information:
http://www.lbl.gov

More articles from Life Sciences:

nachricht Study shines light on brain cells that coordinate movement
26.06.2017 | University of Washington Health Sciences/UW Medicine

nachricht New insight into a central biological dogma on ion transport
26.06.2017 | Aarhus University

All articles from Life Sciences >>>

The most recent press releases about innovation >>>

Die letzten 5 Focus-News des innovations-reports im Überblick:

Im Focus: Can we see monkeys from space? Emerging technologies to map biodiversity

An international team of scientists has proposed a new multi-disciplinary approach in which an array of new technologies will allow us to map biodiversity and the risks that wildlife is facing at the scale of whole landscapes. The findings are published in Nature Ecology and Evolution. This international research is led by the Kunming Institute of Zoology from China, University of East Anglia, University of Leicester and the Leibniz Institute for Zoo and Wildlife Research.

Using a combination of satellite and ground data, the team proposes that it is now possible to map biodiversity with an accuracy that has not been previously...

Im Focus: Climate satellite: Tracking methane with robust laser technology

Heatwaves in the Arctic, longer periods of vegetation in Europe, severe floods in West Africa – starting in 2021, scientists want to explore the emissions of the greenhouse gas methane with the German-French satellite MERLIN. This is made possible by a new robust laser system of the Fraunhofer Institute for Laser Technology ILT in Aachen, which achieves unprecedented measurement accuracy.

Methane is primarily the result of the decomposition of organic matter. The gas has a 25 times greater warming potential than carbon dioxide, but is not as...

Im Focus: How protons move through a fuel cell

Hydrogen is regarded as the energy source of the future: It is produced with solar power and can be used to generate heat and electricity in fuel cells. Empa researchers have now succeeded in decoding the movement of hydrogen ions in crystals – a key step towards more efficient energy conversion in the hydrogen industry of tomorrow.

As charge carriers, electrons and ions play the leading role in electrochemical energy storage devices and converters such as batteries and fuel cells. Proton...

Im Focus: A unique data centre for cosmological simulations

Scientists from the Excellence Cluster Universe at the Ludwig-Maximilians-Universität Munich have establised "Cosmowebportal", a unique data centre for cosmological simulations located at the Leibniz Supercomputing Centre (LRZ) of the Bavarian Academy of Sciences. The complete results of a series of large hydrodynamical cosmological simulations are available, with data volumes typically exceeding several hundred terabytes. Scientists worldwide can interactively explore these complex simulations via a web interface and directly access the results.

With current telescopes, scientists can observe our Universe’s galaxies and galaxy clusters and their distribution along an invisible cosmic web. From the...

Im Focus: Scientists develop molecular thermometer for contactless measurement using infrared light

Temperature measurements possible even on the smallest scale / Molecular ruby for use in material sciences, biology, and medicine

Chemists at Johannes Gutenberg University Mainz (JGU) in cooperation with researchers of the German Federal Institute for Materials Research and Testing (BAM)...

All Focus news of the innovation-report >>>

Anzeige

Anzeige

Event News

Plants are networkers

19.06.2017 | Event News

Digital Survival Training for Executives

13.06.2017 | Event News

Global Learning Council Summit 2017

13.06.2017 | Event News

 
Latest News

Study shines light on brain cells that coordinate movement

26.06.2017 | Life Sciences

Smooth propagation of spin waves using gold

26.06.2017 | Physics and Astronomy

Switchable DNA mini-machines store information

26.06.2017 | Information Technology

VideoLinks
B2B-VideoLinks
More VideoLinks >>>