Forum for Science, Industry and Business

Sponsored by:     3M 
Search our Site:

 

Stem cell microenvironment reverses malignant melanoma

18.11.2005


Northwestern University researchers have demonstrated how the microenvironments of two human embryonic stem cell (hESC) lines (federally approved) induced metastatic melanoma cells to revert to a normal, skin cell-like type with the ability to form colonies similar to hESCs. The researchers also showed that these melanoma cells were less invasive following culture on the microenvironments of hESCs.



"Our observations highlight the potential utility of isolating the factors within the hESC microenvironment responsible for influencing tumor cell fate and reversing the cancerous properties of metastatic tumor cells, such as melanoma," said Mary J. C. Hendrix, in whose laboratories at Children’s Memorial Research Center the experiments were conducted.

An article describing the findings by Hendrix and her laboratory group was published in the Nov. 17 online issue of the journal Stem Cells. Hendrix is president and scientific director of the Children’s Memorial Research Center at Northwestern University Feinberg School of Medicine and a member of the executive committees of The Robert H. Lurie Comprehensive Cancer Center and the Center for Genetic Medicine at Northwestern University. The Northwestern researchers used a unique, three-dimensional model to test whether the microenvironment supporting human embryonic stem cells (hESCs) would influence the behavior of human metastatic melanoma cells – since hESCs have the ability to develop into a variety of normal cell types – to assume a more normal melanocyte-like cell, the skin cell type of origin for melanoma.


The model, which was developed in Hendrix’s laboratories, consists of a three-dimensional collagen matrix preconditioned by hESCs, followed by their removal and subsequent application, or seeding, of metastatic melanoma cells onto the embryonic microenvironment, which was followed by molecular and functional analyses.

The team applied two different hESC lines, independently, onto three-dimensional collagen matrices and allowed the cells to form colonies and precondition their microenvironments for several days. The hESCs were removed and the matrix microenvironments were left intact. Then, human metastatic melanoma cells were seeded onto the hESC-preconditioned matrix microenvironment and were allowed to remain for several days. After this period, the metastatic melanoma cells exposed to the hECS microenvironment were reprogrammed to express a melanocyte-associated protein, called Melan-A, and form colonies similar to the hESC colonies. The melanoma cells reprogrammed by the hESC microenvironment were also less invasive than the tumor cells that had not been exposed to the embryonic matrices.

"These findings offer a new approach to investigating the possible effects of identifying the microenvironmental factors produced by hESCs on reversing the metastatic properties of tumor cells," Hendrix said.

Elizabeth Crown | EurekAlert!
Further information:
http://www.northwestern.edu

More articles from Life Sciences:

nachricht A Map of the Cell’s Power Station
18.08.2017 | Albert-Ludwigs-Universität Freiburg im Breisgau

nachricht On the way to developing a new active ingredient against chronic infections
18.08.2017 | Deutsches Zentrum für Infektionsforschung

All articles from Life Sciences >>>

The most recent press releases about innovation >>>

Die letzten 5 Focus-News des innovations-reports im Überblick:

Im Focus: Fizzy soda water could be key to clean manufacture of flat wonder material: Graphene

Whether you call it effervescent, fizzy, or sparkling, carbonated water is making a comeback as a beverage. Aside from quenching thirst, researchers at the University of Illinois at Urbana-Champaign have discovered a new use for these "bubbly" concoctions that will have major impact on the manufacturer of the world's thinnest, flattest, and one most useful materials -- graphene.

As graphene's popularity grows as an advanced "wonder" material, the speed and quality at which it can be manufactured will be paramount. With that in mind,...

Im Focus: Exotic quantum states made from light: Physicists create optical “wells” for a super-photon

Physicists at the University of Bonn have managed to create optical hollows and more complex patterns into which the light of a Bose-Einstein condensate flows. The creation of such highly low-loss structures for light is a prerequisite for complex light circuits, such as for quantum information processing for a new generation of computers. The researchers are now presenting their results in the journal Nature Photonics.

Light particles (photons) occur as tiny, indivisible portions. Many thousands of these light portions can be merged to form a single super-photon if they are...

Im Focus: Circular RNA linked to brain function

For the first time, scientists have shown that circular RNA is linked to brain function. When a RNA molecule called Cdr1as was deleted from the genome of mice, the animals had problems filtering out unnecessary information – like patients suffering from neuropsychiatric disorders.

While hundreds of circular RNAs (circRNAs) are abundant in mammalian brains, one big question has remained unanswered: What are they actually good for? In the...

Im Focus: RAVAN CubeSat measures Earth's outgoing energy

An experimental small satellite has successfully collected and delivered data on a key measurement for predicting changes in Earth's climate.

The Radiometer Assessment using Vertically Aligned Nanotubes (RAVAN) CubeSat was launched into low-Earth orbit on Nov. 11, 2016, in order to test new...

Im Focus: Scientists shine new light on the “other high temperature superconductor”

A study led by scientists of the Max Planck Institute for the Structure and Dynamics of Matter (MPSD) at the Center for Free-Electron Laser Science in Hamburg presents evidence of the coexistence of superconductivity and “charge-density-waves” in compounds of the poorly-studied family of bismuthates. This observation opens up new perspectives for a deeper understanding of the phenomenon of high-temperature superconductivity, a topic which is at the core of condensed matter research since more than 30 years. The paper by Nicoletti et al has been published in the PNAS.

Since the beginning of the 20th century, superconductivity had been observed in some metals at temperatures only a few degrees above the absolute zero (minus...

All Focus news of the innovation-report >>>

Anzeige

Anzeige

Event News

Call for Papers – ICNFT 2018, 5th International Conference on New Forming Technology

16.08.2017 | Event News

Sustainability is the business model of tomorrow

04.08.2017 | Event News

Clash of Realities 2017: Registration now open. International Conference at TH Köln

26.07.2017 | Event News

 
Latest News

A Map of the Cell’s Power Station

18.08.2017 | Life Sciences

Engineering team images tiny quasicrystals as they form

18.08.2017 | Physics and Astronomy

Researchers printed graphene-like materials with inkjet

18.08.2017 | Materials Sciences

VideoLinks
B2B-VideoLinks
More VideoLinks >>>