Forum for Science, Industry and Business

Sponsored by:     3M 
Search our Site:

 

New purification process joins high throughput with high selectivity

18.05.2005


Penn State chemical engineers have demonstrated proof of concept for a new protein purification process that combines ultrafiltration’s high throughput with high specificity achievable through electrically-charged dyes that bind to the protein.


Ultrafiltration is widely used now in the pharmaceutical industry, by milk and whey producers and in water purification. The new process promises to broaden the scope of ultrafiltration to more fine separations.

In the proof of concept experiments, the protein of interest was tagged with a small, commercially-available, negatively-charged dye molecule that can be easily removed. When the solution to be purified flowed through a negatively-charged ultrafiltration membrane, the protein of interest, now negatively-charged because of the attached dye, was retained in higher proportion than when it wasn’t tagged.

Dr. Andrew Zydney, professor of chemical engineering and a developer of the process, says, "Classically, in ultrafiltration, the size of the pores in the filter determined what could get through. Recent studies have demonstrated that additional retention can be achieved using electrically charged membranes if the protein were of like charge. However, these new experiments have shown that you can enhance retention for the same size pore by attaching a charged dye marker to the protein of interest to change its electrical charge."



The new purification process is detailed in a paper, "Controlling Protein Transport in Ultrafiltration Using Small Charged Ligands," published online in the current issue of the journal, Biotechnology and Bioengineering. The authors are Suma Rao, doctoral candidate in chemical engineering, and Zydney, who is also head of the Department of Chemical Engineering.

For their proof of concept experiments, the researchers chose bovine serum albumin (BSA) and the dye Cibacron Blue. They obtained and compared sieving data from neutral and negatively-charged filters. They found that the addition of about one gram per liter of Cibacron Blue to an eight gram per liter solution of BSA caused a reduction in the BSA sieving coefficient by more than 100 times (two orders of magnitude) when passed through the negatively-charged filter.

The researchers note in their paper that it would be possible to design a system in which the protein and dye were separated after filtering and the dye recycled for subsequent processes. For example, in the case of Cibacron Blue and BSA, decreasing the acidity of the solution and adding certain salts causes the dye to detach readily from the BSA. The dye can then be recovered through filtration.

While the proof of concept experiments were conducted with just one protein, the researchers say that it should be possible to exploit the specificity of dye-binding interactions to enhance the selectivity of other protein separations providing new opportunities for membrane systems.

Barbara Hale | EurekAlert!
Further information:
http://www.psu.edu

More articles from Life Sciences:

nachricht Fingerprint' technique spots frog populations at risk from pollution
27.03.2017 | Lancaster University

nachricht Parallel computation provides deeper insight into brain function
27.03.2017 | Okinawa Institute of Science and Technology (OIST) Graduate University

All articles from Life Sciences >>>

The most recent press releases about innovation >>>

Die letzten 5 Focus-News des innovations-reports im Überblick:

Im Focus: Giant Magnetic Fields in the Universe

Astronomers from Bonn and Tautenburg in Thuringia (Germany) used the 100-m radio telescope at Effelsberg to observe several galaxy clusters. At the edges of these large accumulations of dark matter, stellar systems (galaxies), hot gas, and charged particles, they found magnetic fields that are exceptionally ordered over distances of many million light years. This makes them the most extended magnetic fields in the universe known so far.

The results will be published on March 22 in the journal „Astronomy & Astrophysics“.

Galaxy clusters are the largest gravitationally bound structures in the universe. With a typical extent of about 10 million light years, i.e. 100 times the...

Im Focus: Tracing down linear ubiquitination

Researchers at the Goethe University Frankfurt, together with partners from the University of Tübingen in Germany and Queen Mary University as well as Francis Crick Institute from London (UK) have developed a novel technology to decipher the secret ubiquitin code.

Ubiquitin is a small protein that can be linked to other cellular proteins, thereby controlling and modulating their functions. The attachment occurs in many...

Im Focus: Perovskite edges can be tuned for optoelectronic performance

Layered 2D material improves efficiency for solar cells and LEDs

In the eternal search for next generation high-efficiency solar cells and LEDs, scientists at Los Alamos National Laboratory and their partners are creating...

Im Focus: Polymer-coated silicon nanosheets as alternative to graphene: A perfect team for nanoelectronics

Silicon nanosheets are thin, two-dimensional layers with exceptional optoelectronic properties very similar to those of graphene. Albeit, the nanosheets are less stable. Now researchers at the Technical University of Munich (TUM) have, for the first time ever, produced a composite material combining silicon nanosheets and a polymer that is both UV-resistant and easy to process. This brings the scientists a significant step closer to industrial applications like flexible displays and photosensors.

Silicon nanosheets are thin, two-dimensional layers with exceptional optoelectronic properties very similar to those of graphene. Albeit, the nanosheets are...

Im Focus: Researchers Imitate Molecular Crowding in Cells

Enzymes behave differently in a test tube compared with the molecular scrum of a living cell. Chemists from the University of Basel have now been able to simulate these confined natural conditions in artificial vesicles for the first time. As reported in the academic journal Small, the results are offering better insight into the development of nanoreactors and artificial organelles.

Enzymes behave differently in a test tube compared with the molecular scrum of a living cell. Chemists from the University of Basel have now been able to...

All Focus news of the innovation-report >>>

Anzeige

Anzeige

Event News

International Land Use Symposium ILUS 2017: Call for Abstracts and Registration open

20.03.2017 | Event News

CONNECT 2017: International congress on connective tissue

14.03.2017 | Event News

ICTM Conference: Turbine Construction between Big Data and Additive Manufacturing

07.03.2017 | Event News

 
Latest News

Northern oceans pumped CO2 into the atmosphere

27.03.2017 | Earth Sciences

Fingerprint' technique spots frog populations at risk from pollution

27.03.2017 | Life Sciences

Big data approach to predict protein structure

27.03.2017 | Life Sciences

VideoLinks
B2B-VideoLinks
More VideoLinks >>>