Forum for Science, Industry and Business

Sponsored by:     3M 
Search our Site:

 

Chemical library aids in developing drug system for nerve damage

13.04.2005


A researcher studying drug design for nerve damage therapies has gotten her answer to questions by following some old advice: she used the library.

It’s not the kind of library her mother or teacher suggested, but a combinatorial chemistry library of many different protein sequences that some day might help her and her colleagues develop a successful timed drug delivery system.

Shelly Sakiyama-Elbert, Ph.D., assistant professor of biomedical engineering at Washington University in St. Louis, has screened a large number of molecules to find which ones have varying affinity, or attraction, to a sugar that binds nerve repair drugs called heparin, as well as a nerve repair protein called nerve growth factor.



Sakiyama-Elbert ran a library of viruses called bacteriophage that contained small random portions, or sequences of their surface proteins — which could be used to attract or bind other proteins — through a column with the drug bound to it. She then made the playing field more difficult for the bacteriophage to bind so that eventually she could find bacteriophage peptides that bound to heparin or nerve growth factor. By repeating this process numerous times, she identified peptide sequences that have low, medium or high affinity for the heparin drug.

Looking for good protein sequences

Sakiyama-Elbert and her colleagues are looking for protein sequences that bind to drugs to help a drug delivery vehicle provide timed release of a drug. Such drug delivery systems are called affinity-based, and it is hoped that eventually they will provide the signals necessary to stimulate tissue regeneration for conditions such as nerve damage on an appropriate time scale.

In conjunction with the sequence technique, Sakiyama-Elbert and her group developed a mathematical model that identifies the kind of drug release desired as a basis to narrow down the range of affinities they want to identify from the library. Between the modeling and future experimental studies, they hope to refine their drug delivery design to get the optimal rate of drug release.

"We started with a model I’d previously developed, then added in some features that allow us to model degradation of the delivery system by enzymes," Sakiyama-Elbert said. "Specifically, we added a component where we can model what would happen if there is a cell in part of the delivery system and how that would affect release throughout the delivery system.

"Before you only could address what would happen in a culture dish with no cells around it. We’re really interested in what will happen in cell culture or an animal model where there will be active cell-mediated degradation. We are trying to get closer to the real situation." The results were published in the January 2005 issue of Acta Biomateriali. The work was supported by a grant from the Whitaker Foundation.

Sakiyama-Elbert said lots of researchers are adopting the concept of affinity-based drug delivery systems, and the Washington University library screening technique and mathematical model together provide a good tool to expand the usefulness of these approaches.

"One interesting thing we’ve found in this work is that it appears the activities of the drugs that we’re delivering vary with the affinity of the binding site," she said. "We’re not sure if that’s a function of the affinity controlling the rate of release or if there is actually some separate biological modulator that’s being affected.

"The good thing, though, is that we’ve identified several different affinities of binding sites so we can now test whether it’s the affinity or the rate of release and determine what’s really going on."

This ability is important for researchers to get insight into the biological activity of different drugs and how they might be modulated for drug release.

"We have low, medium and high affinity binding proteins," Sakiyama-Elbert. "We can look at fast and slow release rate for all three of them, so we can control affinity and concentration to our advantage."

Tony Fitzpatrick | EurekAlert!
Further information:
http://www.wustl.edu

More articles from Life Sciences:

nachricht New type of photosynthesis discovered
17.06.2018 | Imperial College London

nachricht New ID pictures of conducting polymers discover a surprise ABBA fan
17.06.2018 | University of Warwick

All articles from Life Sciences >>>

The most recent press releases about innovation >>>

Die letzten 5 Focus-News des innovations-reports im Überblick:

Im Focus: AchemAsia 2019 will take place in Shanghai

Moving into its fourth decade, AchemAsia is setting out for new horizons: The International Expo and Innovation Forum for Sustainable Chemical Production will take place from 21-23 May 2019 in Shanghai, China. With an updated event profile, the eleventh edition focusses on topics that are especially relevant for the Chinese process industry, putting a strong emphasis on sustainability and innovation.

Founded in 1989 as a spin-off of ACHEMA to cater to the needs of China’s then developing industry, AchemAsia has since grown into a platform where the latest...

Im Focus: First real-time test of Li-Fi utilization for the industrial Internet of Things

The BMBF-funded OWICELLS project was successfully completed with a final presentation at the BMW plant in Munich. The presentation demonstrated a Li-Fi communication with a mobile robot, while the robot carried out usual production processes (welding, moving and testing parts) in a 5x5m² production cell. The robust, optical wireless transmission is based on spatial diversity; in other words, data is sent and received simultaneously by several LEDs and several photodiodes. The system can transmit data at more than 100 Mbit/s and five milliseconds latency.

Modern production technologies in the automobile industry must become more flexible in order to fulfil individual customer requirements.

Im Focus: Sharp images with flexible fibers

An international team of scientists has discovered a new way to transfer image information through multimodal fibers with almost no distortion - even if the fiber is bent. The results of the study, to which scientist from the Leibniz-Institute of Photonic Technology Jena (Leibniz IPHT) contributed, were published on 6thJune in the highly-cited journal Physical Review Letters.

Endoscopes allow doctors to see into a patient’s body like through a keyhole. Typically, the images are transmitted via a bundle of several hundreds of optical...

Im Focus: Photoexcited graphene puzzle solved

A boost for graphene-based light detectors

Light detection and control lies at the heart of many modern device applications, such as smartphone cameras. Using graphene as a light-sensitive material for...

Im Focus: Water is not the same as water

Water molecules exist in two different forms with almost identical physical properties. For the first time, researchers have succeeded in separating the two forms to show that they can exhibit different chemical reactivities. These results were reported by researchers from the University of Basel and their colleagues in Hamburg in the scientific journal Nature Communications.

From a chemical perspective, water is a molecule in which a single oxygen atom is linked to two hydrogen atoms. It is less well known that water exists in two...

All Focus news of the innovation-report >>>

Anzeige

Anzeige

VideoLinks
Industry & Economy
Event News

Munich conference on asteroid detection, tracking and defense

13.06.2018 | Event News

2nd International Baltic Earth Conference in Denmark: “The Baltic Sea region in Transition”

08.06.2018 | Event News

ISEKI_Food 2018: Conference with Holistic View of Food Production

05.06.2018 | Event News

 
Latest News

A sprinkle of platinum nanoparticles onto graphene makes brain probes more sensitive

15.06.2018 | Materials Sciences

100 % Organic Farming in Bhutan – a Realistic Target?

15.06.2018 | Ecology, The Environment and Conservation

Perovskite-silicon solar cell research collaboration hits 25.2% efficiency

15.06.2018 | Power and Electrical Engineering

VideoLinks
Science & Research
Overview of more VideoLinks >>>