Forum for Science, Industry and Business

Sponsored by:     3M 
Search our Site:

 

Breath may help diagnose infection

22.03.2005


Researchers from Johns Hopkins University are developing a novel method of testing exhaled breath to detect infection rapidly after potential exposure to a biological warfare agent. They report their findings today at the 2005 ASM Biodefense Research Meeting.



"We want to have a tool that can help in the emergency room or first responders to triage on site so that people who are infected can get treatment first," says Joany Jackman, a researcher on the project. "It’s not so hard to sample breath from many people very quickly as it is to draw blood."

When exposed to disease causing organisms, cells in the body release proteins, called cytokines, to help the immune cells identify and fight the infection. Jackman and her colleagues theorized that cytokines might work their way up through the tissue until eventually they would be exhaled through water vapor in the breath, and could be captured and identified.


"Old medical texts, in the days long before sophisticated diagnostics, would recommend that a doctor check a patient by checking his or her breath, so we knew there must be something to it," says Jackman.

In previous studies, Jackman and her colleagues exposed pigs to different infectious agents and collected breath samples, which they condensed and ran through a mass spectrometer to test for cytokines and other proteins. They were able to detect a strong surge in cytokines in exhaled breath in as little as an hour, long before any visible symptoms appeared.

"However for this technology to be an effective diagnostic, immune markers which appear as a result of exposure to agents should be absent in uninfected populations," says Jackman.

To determine whether the cytokine levels could be differentiated from a healthy population, Jackman and co-investigator Nate Boggs purchased exhaled breath samples from swine housed at a commercial pig farm and analyzed the condensates.

"In all animals, immune markers of infection were at or below the limit of detection, indicating that baseline levels of immune markers in uninfected and apparently healthy populations could be expected to be low or undetectable," says Boggs.

Now that they have shown the concept of exhaled breath diagnostics to be viable, the next step is to move into human testing. Having determined in earlier studies that the early responses of cells to infection vary based on infectious agent, they hope to create cytokine profiles that will help identify specific diseases. They are also working on redesigning the breath collector with an eye towards getting it approved by the Food and Drug Administration.

Jim Sliwa | EurekAlert!
Further information:
http://www.asmusa.org

More articles from Life Sciences:

nachricht Embryonic development: How do limbs develop from cells?
18.05.2018 | Humboldt-Universität zu Berlin

nachricht Reading histone modifications, an oncoprotein is modified in return
18.05.2018 | American Society for Biochemistry and Molecular Biology

All articles from Life Sciences >>>

The most recent press releases about innovation >>>

Die letzten 5 Focus-News des innovations-reports im Überblick:

Im Focus: Explanation for puzzling quantum oscillations has been found

So-called quantum many-body scars allow quantum systems to stay out of equilibrium much longer, explaining experiment | Study published in Nature Physics

Recently, researchers from Harvard and MIT succeeded in trapping a record 53 atoms and individually controlling their quantum state, realizing what is called a...

Im Focus: Dozens of binaries from Milky Way's globular clusters could be detectable by LISA

Next-generation gravitational wave detector in space will complement LIGO on Earth

The historic first detection of gravitational waves from colliding black holes far outside our galaxy opened a new window to understanding the universe. A...

Im Focus: Entangled atoms shine in unison

A team led by Austrian experimental physicist Rainer Blatt has succeeded in characterizing the quantum entanglement of two spatially separated atoms by observing their light emission. This fundamental demonstration could lead to the development of highly sensitive optical gradiometers for the precise measurement of the gravitational field or the earth's magnetic field.

The age of quantum technology has long been heralded. Decades of research into the quantum world have led to the development of methods that make it possible...

Im Focus: Computer-Designed Customized Regenerative Heart Valves

Cardiovascular tissue engineering aims to treat heart disease with prostheses that grow and regenerate. Now, researchers from the University of Zurich, the Technical University Eindhoven and the Charité Berlin have successfully implanted regenerative heart valves, designed with the aid of computer simulations, into sheep for the first time.

Producing living tissue or organs based on human cells is one of the main research fields in regenerative medicine. Tissue engineering, which involves growing...

Im Focus: Light-induced superconductivity under high pressure

A team of scientists of the Max Planck Institute for the Structure and Dynamics of Matter (MPSD) at the Center for Free-Electron Laser Science in Hamburg investigated optically-induced superconductivity in the alkali-doped fulleride K3C60under high external pressures. This study allowed, on one hand, to uniquely assess the nature of the transient state as a superconducting phase. In addition, it unveiled the possibility to induce superconductivity in K3C60 at temperatures far above the -170 degrees Celsius hypothesized previously, and rather all the way to room temperature. The paper by Cantaluppi et al has been published in Nature Physics.

Unlike ordinary metals, superconductors have the unique capability of transporting electrical currents without any loss. Nowadays, their technological...

All Focus news of the innovation-report >>>

Anzeige

Anzeige

VideoLinks
Industry & Economy
Event News

Save the date: Forum European Neuroscience – 07-11 July 2018 in Berlin, Germany

02.05.2018 | Event News

Invitation to the upcoming "Current Topics in Bioinformatics: Big Data in Genomics and Medicine"

13.04.2018 | Event News

Unique scope of UV LED technologies and applications presented in Berlin: ICULTA-2018

12.04.2018 | Event News

 
Latest News

Supersonic waves may help electronics beat the heat

18.05.2018 | Power and Electrical Engineering

Keeping a Close Eye on Ice Loss

18.05.2018 | Information Technology

CrowdWater: An App for Flood Research

18.05.2018 | Information Technology

VideoLinks
Science & Research
Overview of more VideoLinks >>>