Forum for Science, Industry and Business

Sponsored by:     3M 
Search our Site:

 

Mayo Clinic researchers use human antibody to cure malignant melanoma in mice

15.07.2004


Mayo Clinic researchers have manipulated a human antibody to induce an anti-tumor response in living mice that consistently curbs -- and often cures -- malignant melanoma, one of the most lethal forms of skin cancer and the most common cancer of young adults.


In the July 15 edition of Cancer Research Mayo researchers report three innovative discoveries that advance the emerging field of cancer immunotherapy. Cancer immunotherapy refers to scientist-controlled manipulations of the immune system to kill cancer cells without the toxic side effects of chemotherapy or radiation. These findings show that when administered intravenously, the human antibody can still induce immune response -- which suits it for potential therapeutic use as a drug for humans.

"What this current work demonstrates is that by using this antibody we can train the immune response to strike a new target," says Larry Pease, Ph.D., Mayo Clinic immunologist and lead investigator of the study.

The Discoveries



A novel way to fight cancer with the immune system

Treating live mice intravenously with a human antibody stimulates components of the immune system known as dendritic cells, which, in turn, changes the way dendritic cells interact with the T cells of the immune system. The result: a consistently strong -- and often curative -- treatment effect for malignant melanoma, a cancer that is newly diagnosed in approximately 51,000 people in the U.S. annually, and claims more than 7,000 lives a year in the United States.

New approach works to kill cancer in mice

The researchers created an anti-tumor immune response where none existed in nature. Under normal conditions dendritic cells are key players in initiating select immune responses -- responding to malignant melanoma just doesn’t happen to be among them. Mayo Clinic researchers changed that. They trained T cells to seek and destroy malignant melanoma by inducing activity of dendritic cells by "cross-linking" structures on their cell surface. Cross-linking is a molecular manipulation that can stimulate cells.

Effectiveness

In the investigation, one group of mice was treated intravenously with the experimental cross-linking antibody therapy, and two control groups were treated with known antibodies that do not prompt cross-linking structures containing B7-DC. All groups had malignant melanoma tumors transplanted into them. They were then examined 17 days later for evidence of tumor growth.

Results showed that in the two control groups, only one of 26 (less than 4 percent) were tumor free. By contrast, 11 of 16 mice -- 69 percent -- were tumor free in the group receiving the experimental antibody treatment. In addition, the few mice in this group that did develop tumors experienced significantly inhibited tumor growth compared to controls.

In a second line of investigation animals received intravenous transplants of tumors that seeded their lungs with dozens of discrete foci of melanoma, modeling what happens during lung metastasis. After three days, some of these animals were treated with the B7-DC cross-linking antibody or a control antibody. They were evaluated for tumor growth when their untreated counterparts had developed more than 50 tumor nodules in their lungs.

Forty-eight percent of the animals (14 of 29) that received B7-DC cross-linking antibody treatments were tumor free when the experiments were ended three to four weeks after tumor engraftment. In contrast, all the mice that received control antibodies developed large numbers of tumors in their lungs. Furthermore, all of the 52 percent of animals that developed melanoma lung nodules developed substantially fewer tumors relative to the animals receiving irrelevant antibody, showing that treatment with B7-DC cross-linking antibody had a strong treatment effect even when animals were not completely cured.

Distinct from previous methods

The data reveal a dramatic improvement in anti-tumor abilities of dendritic cells stimulated with this human antibody. Following treatment the dendritic cells behaved differently when compared to dendritic cells stimulated by established methods.

"Essentially, we are inducing an immune response against a tumor where an immune response isn’t normally happening," says Dr. Pease. "The human antibody induces strong tumor immunity when administered and without further interventions -- even after tumors are already established. This is an important milestone for any cancer therapy that will be useful for treating patients."

In addition to Dr. Pease, the coauthors of the article include: Suresh Radhakrishnan, Ph.D.; Loc Tan Nguyen; Bogoljub Ciric; Dallas Flies; Virginia P. Van Keulen; Koji Tamada; Lieping Chen and Moses Rodriguez, M.D. Their work was supported by grants from The Ralph C. Wilson, Sr., and Ralph C. Wilson, Jr., Medical Research Foundation, the Mayo Clinic Comprehensive Cancer Center and the National Cancer Institute of the National Institutes of Health.

Bob Nellis | EurekAlert!
Further information:
http://www.mayo.edu
http://cancerres.aacrjournals.org

More articles from Life Sciences:

nachricht What happens in the cell nucleus after fertilization
06.12.2016 | Helmholtz Zentrum München - Deutsches Forschungszentrum für Gesundheit und Umwelt

nachricht Researchers uncover protein-based “cancer signature”
05.12.2016 | Universität Basel

All articles from Life Sciences >>>

The most recent press releases about innovation >>>

Die letzten 5 Focus-News des innovations-reports im Überblick:

Im Focus: Significantly more productivity in USP lasers

In recent years, lasers with ultrashort pulses (USP) down to the femtosecond range have become established on an industrial scale. They could advance some applications with the much-lauded “cold ablation” – if that meant they would then achieve more throughput. A new generation of process engineering that will address this issue in particular will be discussed at the “4th UKP Workshop – Ultrafast Laser Technology” in April 2017.

Even back in the 1990s, scientists were comparing materials processing with nanosecond, picosecond and femtosesecond pulses. The result was surprising:...

Im Focus: Shape matters when light meets atom

Mapping the interaction of a single atom with a single photon may inform design of quantum devices

Have you ever wondered how you see the world? Vision is about photons of light, which are packets of energy, interacting with the atoms or molecules in what...

Im Focus: Novel silicon etching technique crafts 3-D gradient refractive index micro-optics

A multi-institutional research collaboration has created a novel approach for fabricating three-dimensional micro-optics through the shape-defined formation of porous silicon (PSi), with broad impacts in integrated optoelectronics, imaging, and photovoltaics.

Working with colleagues at Stanford and The Dow Chemical Company, researchers at the University of Illinois at Urbana-Champaign fabricated 3-D birefringent...

Im Focus: Quantum Particles Form Droplets

In experiments with magnetic atoms conducted at extremely low temperatures, scientists have demonstrated a unique phase of matter: The atoms form a new type of quantum liquid or quantum droplet state. These so called quantum droplets may preserve their form in absence of external confinement because of quantum effects. The joint team of experimental physicists from Innsbruck and theoretical physicists from Hannover report on their findings in the journal Physical Review X.

“Our Quantum droplets are in the gas phase but they still drop like a rock,” explains experimental physicist Francesca Ferlaino when talking about the...

Im Focus: MADMAX: Max Planck Institute for Physics takes up axion research

The Max Planck Institute for Physics (MPP) is opening up a new research field. A workshop from November 21 - 22, 2016 will mark the start of activities for an innovative axion experiment. Axions are still only purely hypothetical particles. Their detection could solve two fundamental problems in particle physics: What dark matter consists of and why it has not yet been possible to directly observe a CP violation for the strong interaction.

The “MADMAX” project is the MPP’s commitment to axion research. Axions are so far only a theoretical prediction and are difficult to detect: on the one hand,...

All Focus news of the innovation-report >>>

Anzeige

Anzeige

Event News

ICTM Conference 2017: Production technology for turbomachine manufacturing of the future

16.11.2016 | Event News

Innovation Day Laser Technology – Laser Additive Manufacturing

01.11.2016 | Event News

#IC2S2: When Social Science meets Computer Science - GESIS will host the IC2S2 conference 2017

14.10.2016 | Event News

 
Latest News

Simple processing technique could cut cost of organic PV and wearable electronics

06.12.2016 | Materials Sciences

3-D printed kidney phantoms aid nuclear medicine dosing calibration

06.12.2016 | Medical Engineering

Robot on demand: Mobile machining of aircraft components with high precision

06.12.2016 | Power and Electrical Engineering

VideoLinks
B2B-VideoLinks
More VideoLinks >>>