Forum for Science, Industry and Business

Sponsored by:     3M 
Search our Site:

 

Protease-inhibitor cocktail protects, increases anti-microbial action of promising new peptide

12.03.2004


The anti-microbial activity of promising peptides shown in laboratory studies to kill several medically important fungi, some of which are resistant to current drugs, can be enhanced further by protecting the peptides from enzymes programmed to destroy them, University at Buffalo oral biologists have found.



A protease inhibitor cocktail containing compounds that inactivate the enzymes that normally would degrade the small pieces of protein enabled the potential treatments for oral infections to more than double their anti-microbial action, results showed.

Guo-xian Wei, D.D.S., postdoctoral associate in the laboratory of Libuse Bobek, Ph.D., professor of oral biology in UB’s School of Dental Medicine, reported the study findings today (March 11, 2004) at the International Association of Dental Research meeting in Hawaii.


One peptide in particular, called MUC7 12-mer, a piece of a larger, naturally occurring human salivary mucin molecule, has shown particular promise for treating drug-resistant fungal strains, Wei said.

Only a few drugs are available to treat these infections, and some fungal organisms already are resistant, presenting a particular problem for patients with depressed immune systems, such as those with HIV/AIDS, organ-transplant patients and chemotherapy patients.

In earlier research in Bobek’s laboratory, MUC7 12-mer killed fungal agents that cause the most common opportunistic infections that threaten these patients -- candidiasis and cryptococcosis. In addition, the peptide was active in very low concentrations, reducing the likelihood of adverse reactions.

However, when the UB researchers tested MUC7 12-mer in saliva, its potency decreased considerably. They theorized that enzymes, or proteases, in the saliva were breaking down the peptide. To test their theory, they exposed the microbes to the peptide in the presence of saliva and a commercially available protease inhibitor cocktail.

Results showed that MUC7 12-mer killed 96 percent to 99 percent of five different fungal strains in the presence of the protease inhibitors. Without the inhibitors, the peptide killed 18 percent, 21 percent and 40 percent of three strains and approximately 74 percent of two strains. "These results confirm our hypothesis that the PIC protects and increases anti-microbial action of this peptide," said Wei. "Our next step is to see if the peptide-protease inhibitor combination performs equally well in an animal model."


Bobek is co-author on the study, which was supported by a grant from the National Institute of Dental and Craniofacial Research.

The University at Buffalo is a premier research-intensive public university, the largest and most comprehensive campus in the State University of New York. UB’s more than 27,000 students pursue their academic interests through more than 300 undergraduate, graduate and professional degree programs.

Lois Baker | EurekAlert!
Further information:
http://www.buffalo.edu/

More articles from Life Sciences:

nachricht Show me your leaves - Health check for urban trees
12.12.2017 | Gesellschaft für Ökologie e.V.

nachricht Liver Cancer: Lipid Synthesis Promotes Tumor Formation
12.12.2017 | Universität Basel

All articles from Life Sciences >>>

The most recent press releases about innovation >>>

Die letzten 5 Focus-News des innovations-reports im Überblick:

Im Focus: Long-lived storage of a photonic qubit for worldwide teleportation

MPQ scientists achieve long storage times for photonic quantum bits which break the lower bound for direct teleportation in a global quantum network.

Concerning the development of quantum memories for the realization of global quantum networks, scientists of the Quantum Dynamics Division led by Professor...

Im Focus: Electromagnetic water cloak eliminates drag and wake

Detailed calculations show water cloaks are feasible with today's technology

Researchers have developed a water cloaking concept based on electromagnetic forces that could eliminate an object's wake, greatly reducing its drag while...

Im Focus: Scientists channel graphene to understand filtration and ion transport into cells

Tiny pores at a cell's entryway act as miniature bouncers, letting in some electrically charged atoms--ions--but blocking others. Operating as exquisitely sensitive filters, these "ion channels" play a critical role in biological functions such as muscle contraction and the firing of brain cells.

To rapidly transport the right ions through the cell membrane, the tiny channels rely on a complex interplay between the ions and surrounding molecules,...

Im Focus: Towards data storage at the single molecule level

The miniaturization of the current technology of storage media is hindered by fundamental limits of quantum mechanics. A new approach consists in using so-called spin-crossover molecules as the smallest possible storage unit. Similar to normal hard drives, these special molecules can save information via their magnetic state. A research team from Kiel University has now managed to successfully place a new class of spin-crossover molecules onto a surface and to improve the molecule’s storage capacity. The storage density of conventional hard drives could therefore theoretically be increased by more than one hundred fold. The study has been published in the scientific journal Nano Letters.

Over the past few years, the building blocks of storage media have gotten ever smaller. But further miniaturization of the current technology is hindered by...

Im Focus: Successful Mechanical Testing of Nanowires

With innovative experiments, researchers at the Helmholtz-Zentrums Geesthacht and the Technical University Hamburg unravel why tiny metallic structures are extremely strong

Light-weight and simultaneously strong – porous metallic nanomaterials promise interesting applications as, for instance, for future aeroplanes with enhanced...

All Focus news of the innovation-report >>>

Anzeige

Anzeige

Event News

See, understand and experience the work of the future

11.12.2017 | Event News

Innovative strategies to tackle parasitic worms

08.12.2017 | Event News

AKL’18: The opportunities and challenges of digitalization in the laser industry

07.12.2017 | Event News

 
Latest News

Long-lived storage of a photonic qubit for worldwide teleportation

12.12.2017 | Physics and Astronomy

Multi-year submarine-canyon study challenges textbook theories about turbidity currents

12.12.2017 | Earth Sciences

Electromagnetic water cloak eliminates drag and wake

12.12.2017 | Power and Electrical Engineering

VideoLinks
B2B-VideoLinks
More VideoLinks >>>