Forum for Science, Industry and Business

Sponsored by:     3M 
Search our Site:

 

Cord Blood Cells Proven to Differentiate Into Heart Muscle and Brain Cells

17.02.2004


Joanne Kurtzberg, MD
Credit: Duke University Medical Center


Scientists at the Duke Comprehensive Cancer Center have scientifically validated for the first time that stem cells in umbilical cord blood can infiltrate damaged heart tissue and transform themselves into the kind of heart cells needed to halt further damage.

Clinical proof of this principle has existed for a decade, as Duke physicians have used cord blood to correct heart, brain and liver defects in children with rare metabolic diseases. But until now they lacked the molecular evidence to prove that cord blood stem cells were the root of a cure.

Now, the Duke team has dissected heart tissue to confirm the presence of donor stem cells in heart tissue. Moreover, they showed that donor stem cells had differentiated into heart muscle cells called myocytes, which then produced the critical enzyme needed to halt the progressive heart damage, said Kirsten Crapnell, Ph.D., a research fellow at Duke.



Crapnell will present the team’s findings at the International Association of Bone Marrow Transplantation Research meeting Feb. 12-17 in Orlando, Fla.

"We’ve had convincing clinical evidence that stem cells from umbilical cord blood extended much farther than the blood-forming and immune systems, and that they can differentiate themselves into brain, heart, liver and bone cells," said Joanne Kurtzberg, M.D., director of the Duke Pediatric Bone Marrow and Stem Cell Transplant Program. "But now we have examined heart tissue on a cellular level and proven that donor cells are not only present in heart tissue, but they have become heart muscle cells."

To validate the stem cells’ activities, Crapnell dissected and analyzed heart tissue from a 4-year-old boy whose transplant was successful, but who later died of an infection before his immune system was strong enough to fight it. The boy had suffered from a rare metabolic disease called Sanfilippo Syndrome B, in which the body is missing a critical enzyme needed to break down complex sugars in various cells. As sugar byproducts accumulate in vital organs such as the liver, heart and brain, cells become damaged and die.

Duke physicians had observed that children with these rare metabolic diseases tended to regain organ function more rapidly when given cord blood rather than traditional bone marrow. They theorized that cord blood stem cells, being less mature than stem cells in adult bone marrow, could more easily adapt to their new surroundings and respond to signals that differentiate them into the needed kind of cell.

Indeed, it appears that donor stem cells inexplicably home in on defective tissue and establish themselves there, as though they are missionaries recruited to rescue cells in need, the scientists said. Proof of this principle has come from various imaging studies which demonstrate that certain organs regain function after cord blood transplants. But no one had shown that cord blood cells were actually present in these organs and the reason behind the improvements -- until now.

Crapnell proved the presence of donor stem cells in the young boy’s heart by searching for aberrant female stem cells (from his baby girl donor) amid his largely male heart cells. She used two fluorescent stains to label heart cells as either male or female. The red stain would be attracted to female X chromosomes and the green stain would be attracted to male Y chromosomes. The fluorescent colors would easily illuminate the gender of each cell.

Crapnell then used two additional antibody stains to test for cell surface "markers" -- sites on the surfaces of cells -- that indicate what type of cell it is. The first stain had an affinity for troponin, a protein on the surface of heart cells. The second stain had an affinity for myosin, a protein on the surface of muscle cells. Again, she could easily label each cell as a heart muscle cell or one of another origin.

She found that, although few in number, the female heart cells were clearly illuminated amid a larger pool of male heart cells (called myocytes). Just a few donor stem cells are enough to provide a wide swath of damaged tissue with the enzyme necessary to restore function, the researchers believe.

Cord blood stem cells differentiate into brain cells

The same phenomenon is likely at work in brain tissue as well, said Jennifer Hall, MD, a research fellow at Duke who is also presenting at the International Association of Bone Marrow Transplantation Research meeting.

Cord blood transplants appear to halt or slow the progressive brain damage that is caused by metabolic diseases such as Sanfilippo Syndrome, Krabbe Disease and Hurler’s Syndrome. Yet precious time is lost as the stem cells traverse their way from the bloodstream into the brain, where they eventually engraft and differentiate into the needed types of brain cells. Meanwhile, children may miss a critical therapeutic window of treatment.

If scientists could somehow nudge stem cells toward becoming the needed brain cells, then deliver them directly to the patient’s brain in their differentiated state, doctors could theoretically spare some patients irreversible brain damage.

So Hall tested the potential of hematopoietic stem cells to differentiate inside a test tube into specific kinds of brain cells, called oligodendroctyes. These brain cells are targeted for destruction in children with metabolic diseases.

Hall cultured a unit of cord blood stem cells in a flask together with growth factors, hormones and other compounds that direct stem cells toward the oligodendroctye lineage. One month later, Hall analyzed the cells under a microscope and found that 60 percent of them appeared to resemble cells of an oligodendrocyte lineage.

Hall validated her observations by staining the cells with various antibodies that only bind to and illuminate proteins unique to oligodendrocytes precursor cells. Similarly, she stained the cells with antibodies for a host of other cell types. The presence of unique proteins in a given cell confirms that it is actively producing that protein, not just that its genetic code is capable of doing so, said Hall. In this case, the oligodendrocytes were producing the needed protein or enzyme.

"The therapeutic goal is to rapidly produce oligodendrocytes in the lab, and then infuse them into patients soon after transplant," said Hall. "Delivering cells directly to the brain could hasten engraftment of the cells and could ultimately result in repairing of neurologic tissue."

Hall said the potential also exists for repairing spinal cord injuries and multiple sclerosis, which -- like the metabolic "leukodystrophies" -- result from deterioration of the myelin sheath that coats nerve cells.

Becky Levine | dukemed news
Further information:
http://dukemednews.org/news/article.php?id=7404

More articles from Life Sciences:

nachricht More genes are active in high-performance maize
19.01.2018 | Rheinische Friedrich-Wilhelms-Universität Bonn

nachricht How plants see light
19.01.2018 | Albert-Ludwigs-Universität Freiburg im Breisgau

All articles from Life Sciences >>>

The most recent press releases about innovation >>>

Die letzten 5 Focus-News des innovations-reports im Überblick:

Im Focus: Artificial agent designs quantum experiments

On the way to an intelligent laboratory, physicists from Innsbruck and Vienna present an artificial agent that autonomously designs quantum experiments. In initial experiments, the system has independently (re)discovered experimental techniques that are nowadays standard in modern quantum optical laboratories. This shows how machines could play a more creative role in research in the future.

We carry smartphones in our pockets, the streets are dotted with semi-autonomous cars, but in the research laboratory experiments are still being designed by...

Im Focus: Scientists decipher key principle behind reaction of metalloenzymes

So-called pre-distorted states accelerate photochemical reactions too

What enables electrons to be transferred swiftly, for example during photosynthesis? An interdisciplinary team of researchers has worked out the details of how...

Im Focus: The first precise measurement of a single molecule's effective charge

For the first time, scientists have precisely measured the effective electrical charge of a single molecule in solution. This fundamental insight of an SNSF Professor could also pave the way for future medical diagnostics.

Electrical charge is one of the key properties that allows molecules to interact. Life itself depends on this phenomenon: many biological processes involve...

Im Focus: Paradigm shift in Paris: Encouraging an holistic view of laser machining

At the JEC World Composite Show in Paris in March 2018, the Fraunhofer Institute for Laser Technology ILT will be focusing on the latest trends and innovations in laser machining of composites. Among other things, researchers at the booth shared with the Aachen Center for Integrative Lightweight Production (AZL) will demonstrate how lasers can be used for joining, structuring, cutting and drilling composite materials.

No other industry has attracted as much public attention to composite materials as the automotive industry, which along with the aerospace industry is a driver...

Im Focus: Room-temperature multiferroic thin films and their properties

Scientists at Tokyo Institute of Technology (Tokyo Tech) and Tohoku University have developed high-quality GFO epitaxial films and systematically investigated their ferroelectric and ferromagnetic properties. They also demonstrated the room-temperature magnetocapacitance effects of these GFO thin films.

Multiferroic materials show magnetically driven ferroelectricity. They are attracting increasing attention because of their fascinating properties such as...

All Focus news of the innovation-report >>>

Anzeige

Anzeige

Event News

10th International Symposium: “Advanced Battery Power – Kraftwerk Batterie” Münster, 10-11 April 2018

08.01.2018 | Event News

See, understand and experience the work of the future

11.12.2017 | Event News

Innovative strategies to tackle parasitic worms

08.12.2017 | Event News

 
Latest News

Let the good tubes roll

19.01.2018 | Materials Sciences

How cancer metastasis happens: Researchers reveal a key mechanism

19.01.2018 | Health and Medicine

Meteoritic stardust unlocks timing of supernova dust formation

19.01.2018 | Physics and Astronomy

VideoLinks
B2B-VideoLinks
More VideoLinks >>>