Forum for Science, Industry and Business

Sponsored by:     3M 
Search our Site:


The beginning of the end of flagella


Protein discovery in Chlamydomonas

A new protein discovery sheds light on how chemical information is transported within cells. A group of researchers, which includes Dartmouth Professor of Biological Sciences Roger Sloboda, have found the protein EB1 in Chlamydomonas, a single-celled organism commonly used to study cell biology. Previous research has implicated EB1 in the progression of many colon cancers.

Published in the November 11 edition of the journal Current Biology, the research examined the chemical motors that power events in flagella, antenna-like structures on some cells. Specifically, the research focused on intraflagellar transport (IFT), the process where proteins required for flagellar growth and maintenance move within the flagella. The discovery of the protein EB1 at the tip of the flagella on Chlamydomonas furthers investigations into the role the protein plays in flagellar function and perhaps in regulating IFT itself.

"Particles move out to the tip of the flagella, turn around, and then move back to the base," says Sloboda, who conducted this research in Joel Rosenbaum’s laboratory at Yale University while on sabbatical last year. "The only change in speed or direction occurs when the particles reach the tip. Now we think EB1 might play a role in controlling the molecular transport system responsible for IFT when the particles reach the tip. This finding will help us get a handle on what’s going on at the tip of the flagellum."

The flagella beat rhythmically, moving the organism, and are made of nine double strands of microtubules and a central pair. According to Sloboda, similar IFT phenomena also take place in rod and cone cells of the human retina, in human kidney cells, and in nerve cells.

To determine where EB1 occurs in Chlamydomonas cells, the researchers cloned and sequenced the protein to make antibodies specific for EB1. The researchers found that the antibodies bound to the flagella tips, indicating that EB1 stays at the tip, and does not move along the length of the flagella.

"This unexpected observation led to the paper being featured on the cover of the journal," says Sloboda. "It was a great result, because now we know more about the structure of the flagellar tip due to the presence of EB1. Using EB1 as bait, we can move on to fish out other proteins that associate with EB1 and learn how together these proteins are involved in tip structure and function and the process of IFT. Hopefully, our work will inform others working on colon cancer, kidney disease, vision, and central nervous system disorders such as Alzheimer’s and Lou Gehrig’s diseases."

The other authors on the paper include Lotte Pedersen, a postdoctoral fellow at Yale University; Stefan Geimer, then a postdoctoral fellow at Yale University and now at the Institut Universität zu Köln in Cologne, Germany; and Joel Rosenbaum, Professor of Molecular, Cellular, and Developmental Biology at Yale University.

The study was funded by the National Institutes of Health, a fellowship from the Deutsche Forschungsgemeinschaft, and the Ira Allen Eastman (Class of 1829) Professorship at Dartmouth, which was established in 1910 through a gift to the College by his widow, Jane Eastman.

Sue Knapp | EurekAlert!
Further information:

More articles from Life Sciences:

nachricht North and South Cooperation to Combat Tuberculosis
22.03.2018 | Universität Zürich

nachricht Researchers Discover New Anti-Cancer Protein
22.03.2018 | Universität Basel

All articles from Life Sciences >>>

The most recent press releases about innovation >>>

Die letzten 5 Focus-News des innovations-reports im Überblick:

Im Focus: Researchers Discover New Anti-Cancer Protein

An international team of researchers has discovered a new anti-cancer protein. The protein, called LHPP, prevents the uncontrolled proliferation of cancer cells in the liver. The researchers led by Prof. Michael N. Hall from the Biozentrum, University of Basel, report in “Nature” that LHPP can also serve as a biomarker for the diagnosis and prognosis of liver cancer.

The incidence of liver cancer, also known as hepatocellular carcinoma, is steadily increasing. In the last twenty years, the number of cases has almost doubled...

Im Focus: Researchers at Fraunhofer monitor re-entry of Chinese space station Tiangong-1

In just a few weeks from now, the Chinese space station Tiangong-1 will re-enter the Earth's atmosphere where it will to a large extent burn up. It is possible that some debris will reach the Earth's surface. Tiangong-1 is orbiting the Earth uncontrolled at a speed of approx. 29,000 km/h.Currently the prognosis relating to the time of impact currently lies within a window of several days. The scientists at Fraunhofer FHR have already been monitoring Tiangong-1 for a number of weeks with their TIRA system, one of the most powerful space observation radars in the world, with a view to supporting the German Space Situational Awareness Center and the ESA with their re-entry forecasts.

Following the loss of radio contact with Tiangong-1 in 2016 and due to the low orbital height, it is now inevitable that the Chinese space station will...

Im Focus: Alliance „OLED Licht Forum“ – Key partner for OLED lighting solutions

Fraunhofer Institute for Organic Electronics, Electron Beam and Plasma Technology FEP, provider of research and development services for OLED lighting solutions, announces the founding of the “OLED Licht Forum” and presents latest OLED design and lighting solutions during light+building, from March 18th – 23rd, 2018 in Frankfurt a.M./Germany, at booth no. F91 in Hall 4.0.

They are united in their passion for OLED (organic light emitting diodes) lighting with all of its unique facets and application possibilities. Thus experts in...

Im Focus: Mars' oceans formed early, possibly aided by massive volcanic eruptions

Oceans formed before Tharsis and evolved together, shaping climate history of Mars

A new scenario seeking to explain how Mars' putative oceans came and went over the last 4 billion years implies that the oceans formed several hundred million...

Im Focus: Tiny implants for cells are functional in vivo

For the first time, an interdisciplinary team from the University of Basel has succeeded in integrating artificial organelles into the cells of live zebrafish embryos. This innovative approach using artificial organelles as cellular implants offers new potential in treating a range of diseases, as the authors report in an article published in Nature Communications.

In the cells of higher organisms, organelles such as the nucleus or mitochondria perform a range of complex functions necessary for life. In the networks of...

All Focus news of the innovation-report >>>



Industry & Economy
Event News

Virtual reality conference comes to Reutlingen

19.03.2018 | Event News

Ultrafast Wireless and Chip Design at the DATE Conference in Dresden

16.03.2018 | Event News

International Tinnitus Conference of the Tinnitus Research Initiative in Regensburg

13.03.2018 | Event News

Latest News

Custom sequences for polymers using visible light

22.03.2018 | Materials Sciences

Scientists develop tiny tooth-mounted sensors that can track what you eat

22.03.2018 | Health and Medicine

Mat baits, hooks and destroys pollutants in water

22.03.2018 | Earth Sciences

Science & Research
Overview of more VideoLinks >>>