Forum for Science, Industry and Business

Sponsored by:     3M 
Search our Site:

 

The beginning of the end of flagella

21.11.2003


Protein discovery in Chlamydomonas



A new protein discovery sheds light on how chemical information is transported within cells. A group of researchers, which includes Dartmouth Professor of Biological Sciences Roger Sloboda, have found the protein EB1 in Chlamydomonas, a single-celled organism commonly used to study cell biology. Previous research has implicated EB1 in the progression of many colon cancers.

Published in the November 11 edition of the journal Current Biology, the research examined the chemical motors that power events in flagella, antenna-like structures on some cells. Specifically, the research focused on intraflagellar transport (IFT), the process where proteins required for flagellar growth and maintenance move within the flagella. The discovery of the protein EB1 at the tip of the flagella on Chlamydomonas furthers investigations into the role the protein plays in flagellar function and perhaps in regulating IFT itself.


"Particles move out to the tip of the flagella, turn around, and then move back to the base," says Sloboda, who conducted this research in Joel Rosenbaum’s laboratory at Yale University while on sabbatical last year. "The only change in speed or direction occurs when the particles reach the tip. Now we think EB1 might play a role in controlling the molecular transport system responsible for IFT when the particles reach the tip. This finding will help us get a handle on what’s going on at the tip of the flagellum."

The flagella beat rhythmically, moving the organism, and are made of nine double strands of microtubules and a central pair. According to Sloboda, similar IFT phenomena also take place in rod and cone cells of the human retina, in human kidney cells, and in nerve cells.

To determine where EB1 occurs in Chlamydomonas cells, the researchers cloned and sequenced the protein to make antibodies specific for EB1. The researchers found that the antibodies bound to the flagella tips, indicating that EB1 stays at the tip, and does not move along the length of the flagella.

"This unexpected observation led to the paper being featured on the cover of the journal," says Sloboda. "It was a great result, because now we know more about the structure of the flagellar tip due to the presence of EB1. Using EB1 as bait, we can move on to fish out other proteins that associate with EB1 and learn how together these proteins are involved in tip structure and function and the process of IFT. Hopefully, our work will inform others working on colon cancer, kidney disease, vision, and central nervous system disorders such as Alzheimer’s and Lou Gehrig’s diseases."


The other authors on the paper include Lotte Pedersen, a postdoctoral fellow at Yale University; Stefan Geimer, then a postdoctoral fellow at Yale University and now at the Institut Universität zu Köln in Cologne, Germany; and Joel Rosenbaum, Professor of Molecular, Cellular, and Developmental Biology at Yale University.

The study was funded by the National Institutes of Health, a fellowship from the Deutsche Forschungsgemeinschaft, and the Ira Allen Eastman (Class of 1829) Professorship at Dartmouth, which was established in 1910 through a gift to the College by his widow, Jane Eastman.

Sue Knapp | EurekAlert!
Further information:
http://www.dartmouth.edu/

More articles from Life Sciences:

nachricht Studying mitosis' structure to understand the inside of cancer cells
19.02.2018 | Biophysical Society

nachricht Calcium may play a role in the development of Parkinson's disease
19.02.2018 | University of Cambridge

All articles from Life Sciences >>>

The most recent press releases about innovation >>>

Die letzten 5 Focus-News des innovations-reports im Überblick:

Im Focus: In best circles: First integrated circuit from self-assembled polymer

For the first time, a team of researchers at the Max-Planck Institute (MPI) for Polymer Research in Mainz, Germany, has succeeded in making an integrated circuit (IC) from just a monolayer of a semiconducting polymer via a bottom-up, self-assembly approach.

In the self-assembly process, the semiconducting polymer arranges itself into an ordered monolayer in a transistor. The transistors are binary switches used...

Im Focus: Demonstration of a single molecule piezoelectric effect

Breakthrough provides a new concept of the design of molecular motors, sensors and electricity generators at nanoscale

Researchers from the Institute of Organic Chemistry and Biochemistry of the CAS (IOCB Prague), Institute of Physics of the CAS (IP CAS) and Palacký University...

Im Focus: Hybrid optics bring color imaging using ultrathin metalenses into focus

For photographers and scientists, lenses are lifesavers. They reflect and refract light, making possible the imaging systems that drive discovery through the microscope and preserve history through cameras.

But today's glass-based lenses are bulky and resist miniaturization. Next-generation technologies, such as ultrathin cameras or tiny microscopes, require...

Im Focus: Stem cell divisions in the adult brain seen for the first time

Scientists from the University of Zurich have succeeded for the first time in tracking individual stem cells and their neuronal progeny over months within the intact adult brain. This study sheds light on how new neurons are produced throughout life.

The generation of new nerve cells was once thought to taper off at the end of embryonic development. However, recent research has shown that the adult brain...

Im Focus: Interference as a new method for cooling quantum devices

Theoretical physicists propose to use negative interference to control heat flow in quantum devices. Study published in Physical Review Letters

Quantum computer parts are sensitive and need to be cooled to very low temperatures. Their tiny size makes them particularly susceptible to a temperature...

All Focus news of the innovation-report >>>

Anzeige

Anzeige

VideoLinks
Industry & Economy
Event News

2nd International Conference on High Temperature Shape Memory Alloys (HTSMAs)

15.02.2018 | Event News

Aachen DC Grid Summit 2018

13.02.2018 | Event News

How Global Climate Policy Can Learn from the Energy Transition

12.02.2018 | Event News

 
Latest News

Japanese researchers develop ultrathin, highly elastic skin display

19.02.2018 | Information Technology

Dispersal of Fish Eggs by Water Birds – Just a Myth?

19.02.2018 | Ecology, The Environment and Conservation

Studying mitosis' structure to understand the inside of cancer cells

19.02.2018 | Life Sciences

VideoLinks
Science & Research
Overview of more VideoLinks >>>