Forum for Science, Industry and Business

Sponsored by:     3M 
Search our Site:

 

Researchers Identify New Cause of Genomic Instability

08.08.2003


HHMI Investigator Frederick Alt


Researchers sifting through the indispensable machinery that senses and fixes broken DNA have discovered a new culprit that can induce instability in the genome and thereby set the stage for cancer to develop.

Studies in mice have shown that loss of H2AX, a gene that produces a protein called a histone that is part of the chromosomal structure, can tip the delicate balance of proteins that are curators of the human genome. When H2AX ceases to function properly, lymphomas and solid tumors can arise because errors in the genetic code are not always repaired correctly, according to the new research.

The finding may have important implications for understanding the origin of human cancers because a large number of human tumors are known to contain alterations in the region of chromosome 11 where the H2AX gene is located.



The research was reported in an article published in the August 8, 2003, issue of the journal Cell by Howard Hughes Medical Institute investigator Frederick Alt and colleagues at Children’s Hospital in Boston and Harvard Medical School. Other co-authors are from the Tufts University School of Veterinary Medicine and Brigham and Women’s Hospital.

According to Alt, previous studies by other researchers had shown that H2AX was activated when DNA breaks occur. DNA repair proteins fix genetic damage, but they are also called to action during the normal gene rearrangement that occurs in immune cells when they are readying to battle viruses and other threats.

To explore the implications of knocking out the H2AX gene, lead author Craig Bassing created a line of mice lacking both copies of the H2AX gene. “Both Craig in our lab and Andre Nussenzweig at the National Cancer Institute produced knockout strains that showed an increased level of genomic instability,” said Alt. Nussenzweig and his colleagues have published an article in the same issue of Cell on studies of their H2AX-knockout mice, and have found similar increases in genomic instability and cancer.

According to Alt, the mice lacking only H2AX genes had only a modest increase in cancer, “which is often the case for many genes that produce cancer, because they operate within a system of cellular checks and balances,” he said. “But when you eliminate two genes that may work in concert to maintain good genomic order, you see things happen that are much more dramatic.”

Thus, the researchers created a double-knockout mouse that lacked both H2AX and p53 — a gene that produces a molecular sentinel protein that suppresses proliferation of cells with damaged DNA. In previous studies, Alt and his colleagues had shown that loss of p53 in cells that lacked the DNA-repair process known as non-homologous end-joining (NHEJ) resulted in a dramatic increase in cancers.

“When we deleted both copies of H2AX and both copies of p53, we found a dramatically increased rate of tumors appearing beyond what would be seen with H2AX deficiency alone, and far, far beyond p53 deficiency alone,” said Alt. These cancers developed so rapidly that within a few months all the mice had died, he said. The resulting tumors included both lymphomas arising from aberrant immune cells — which would be common in the loss of NHEJ DNA repair function — and solid tumors, which are not normally seen when NHEJ is compromised.

The researchers also saw cancers arising from malfunction of DNA repair in mature immune cells called B cells. “These kinds of tumors are much more relevant to what goes on in a very large percentage of lymphomas in humans, and adults, in particular,” said Alt.

Alt said that the biggest surprise came when the researchers produced mice missing only one of the two copies of the H2AX gene. “Both surprising and potentially very significant for human cancers was that p53-deficient mice with deletion of one of their two copies of the H2AX gene came down with cancer much earlier,” he said. “They showed a very broad spectrum of tumors that was somewhat different than p53-deficient animals missing both H2AX genes.”

Furthermore, the studies showed that otherwise normal cells that were missing just one H2AX gene had only half the levels of H2AX and also showed genomic instability.

The possibility that only half the levels of the H2AX protein — called “haploinsufficiency” — triggers genomic instability and cancers could be highly significant, said Alt. Such a class of mutation would not have been readily detected in most searches for genes that suppress tumors in humans. Indeed, Bassing discovered that tumors in these mice still had a functioning H2AX gene.

“A major question is why haploinsufficiency of the H2AX protein can cause genomic instability and cancer,” said Alt. “One might explain that quite easily because the protein is not an enzyme, it’s a structural protein. So, if there’s half as much present, it could cause problems in monitoring breaks in the DNA and in recruiting components of the repair machinery.”

What may be especially relevant to human cancers, said Alt, is that the H2AX gene is located in a region of chromosome 11 known to be altered in many human tumors. While there are other potential cancer-causing genes in that region, he said, the current evidence from the mouse model indicates that H2AX will very likely prove a major player in cancer-causing genomic instability.

Alt and his colleagues are now collaborating with scientists at the Dana-Farber Cancer Institute to analyze the status of H2AX genes in a wide range of human cancers. “We believe that the loss of H2AX could prove a major source of the rampant instability associated with the progression of a variety of different tumors,” he said.

Contact: Jim Keeley, mailto:keeleyj@hhmi.org

Jim Keeley | Howard Hughes Medical Institute
Further information:
http://www.hhmi.org

More articles from Life Sciences:

nachricht Ion treatments for cardiac arrhythmia — Non-invasive alternative to catheter-based surgery
20.01.2017 | GSI Helmholtzzentrum für Schwerionenforschung GmbH

nachricht Seeking structure with metagenome sequences
20.01.2017 | DOE/Joint Genome Institute

All articles from Life Sciences >>>

The most recent press releases about innovation >>>

Die letzten 5 Focus-News des innovations-reports im Überblick:

Im Focus: Traffic jam in empty space

New success for Konstanz physicists in studying the quantum vacuum

An important step towards a completely new experimental access to quantum physics has been made at University of Konstanz. The team of scientists headed by...

Im Focus: How gut bacteria can make us ill

HZI researchers decipher infection mechanisms of Yersinia and immune responses of the host

Yersiniae cause severe intestinal infections. Studies using Yersinia pseudotuberculosis as a model organism aim to elucidate the infection mechanisms of these...

Im Focus: Interfacial Superconductivity: Magnetic and superconducting order revealed simultaneously

Researchers from the University of Hamburg in Germany, in collaboration with colleagues from the University of Aarhus in Denmark, have synthesized a new superconducting material by growing a few layers of an antiferromagnetic transition-metal chalcogenide on a bismuth-based topological insulator, both being non-superconducting materials.

While superconductivity and magnetism are generally believed to be mutually exclusive, surprisingly, in this new material, superconducting correlations...

Im Focus: Studying fundamental particles in materials

Laser-driving of semimetals allows creating novel quasiparticle states within condensed matter systems and switching between different states on ultrafast time scales

Studying properties of fundamental particles in condensed matter systems is a promising approach to quantum field theory. Quasiparticles offer the opportunity...

Im Focus: Designing Architecture with Solar Building Envelopes

Among the general public, solar thermal energy is currently associated with dark blue, rectangular collectors on building roofs. Technologies are needed for aesthetically high quality architecture which offer the architect more room for manoeuvre when it comes to low- and plus-energy buildings. With the “ArKol” project, researchers at Fraunhofer ISE together with partners are currently developing two façade collectors for solar thermal energy generation, which permit a high degree of design flexibility: a strip collector for opaque façade sections and a solar thermal blind for transparent sections. The current state of the two developments will be presented at the BAU 2017 trade fair.

As part of the “ArKol – development of architecturally highly integrated façade collectors with heat pipes” project, Fraunhofer ISE together with its partners...

All Focus news of the innovation-report >>>

Anzeige

Anzeige

Event News

Sustainable Water use in Agriculture in Eastern Europe and Central Asia

19.01.2017 | Event News

12V, 48V, high-voltage – trends in E/E automotive architecture

10.01.2017 | Event News

2nd Conference on Non-Textual Information on 10 and 11 May 2017 in Hannover

09.01.2017 | Event News

 
Latest News

Helmholtz International Fellow Award for Sarah Amalia Teichmann

20.01.2017 | Awards Funding

An innovative high-performance material: biofibers made from green lacewing silk

20.01.2017 | Materials Sciences

Ion treatments for cardiac arrhythmia — Non-invasive alternative to catheter-based surgery

20.01.2017 | Life Sciences

VideoLinks
B2B-VideoLinks
More VideoLinks >>>