Forum for Science, Industry and Business

Sponsored by:     3M 
Search our Site:


Bucket with two ears catches DNA


Dutch PhD student Cathelijne Kloks has discovered that the so-called Cold Shock domain of the human YB-1 protein looks like a bucket with two extra ears. These ears lead the DNA to the binding site on the protein and keep it there.

Kloks investigated the structure and function of one of the three domains of the human protein YB-1. This protein plays an important role in the production of new proteins. The central domain, the so-called Cold Shock domain, ensures the binding of the protein to the DNA in the process.

The researcher from the University of Nijmegen discovered that the domain looks like a bucket with a handle and two extra ears. The ears attach to the DNA and push it to the binding site on the YB-1. This binding site was found to be located precisely in between the two ears. This means that the ears can hold the DNA firmly in place whilst it is being bound to the protein. The function of the handle is not yet clear.

Kloks dissolved the YB-1 protein and then studied the solution using NMR measurements. She used the NMR signals to draw up a distances table, which indicated the distance between the nuclei of atoms in the protein. With this information she then calculated the structure of the Cold Shock domain.

Furthermore, Kloks determined the strength of the binding to the DNA. The Cold Shock domain alone formed weak bonds to the DNA. This did not agree with previously made measurements of the binding strength of the complete YB-1 protein. The domain also exhibited little preference with respect to where it binds to the DNA, although previous experiments had shown that the Cold Shock domain binds more strongly to areas of DNA containing a lot of cytosine and thymine. Kloks concluded that the protein’s considerable binding strength and preference could only be clarified by including its other two domains.

In the cell YB-1 forms the link between the transcription of the DNA and the subsequent production of a new protein. YB-1 consists of three different domains. These are compact parts which can fold independently without using other parts of the protein.

The Cold Shock domain derives its name from its function in bacteria. In bacterial proteins the domain ensures that the bacteria resume growth, following a period of arrested growth due to a sudden drop in temperature.

For further information please contact Cathelijne Kloks (Department of Medicinal Chemistry, Organon) tel. +31 (0)412 662461 e-mail: The doctoral thesis will be defended on 26 May 2003. Ms Kloks’’ supervisor is Prof. C.W. Hilbers (University of Nijmegen).

Nalinie Moerlie | alfa
Further information:

More articles from Life Sciences:

nachricht Novel mechanisms of action discovered for the skin cancer medication Imiquimod
21.10.2016 | Technische Universität München

nachricht Second research flight into zero gravity
21.10.2016 | Universität Zürich

All articles from Life Sciences >>>

The most recent press releases about innovation >>>

Die letzten 5 Focus-News des innovations-reports im Überblick:

Im Focus: New 3-D wiring technique brings scalable quantum computers closer to reality

Researchers from the Institute for Quantum Computing (IQC) at the University of Waterloo led the development of a new extensible wiring technique capable of controlling superconducting quantum bits, representing a significant step towards to the realization of a scalable quantum computer.

"The quantum socket is a wiring method that uses three-dimensional wires based on spring-loaded pins to address individual qubits," said Jeremy Béjanin, a PhD...

Im Focus: Scientists develop a semiconductor nanocomposite material that moves in response to light

In a paper in Scientific Reports, a research team at Worcester Polytechnic Institute describes a novel light-activated phenomenon that could become the basis for applications as diverse as microscopic robotic grippers and more efficient solar cells.

A research team at Worcester Polytechnic Institute (WPI) has developed a revolutionary, light-activated semiconductor nanocomposite material that can be used...

Im Focus: Diamonds aren't forever: Sandia, Harvard team create first quantum computer bridge

By forcefully embedding two silicon atoms in a diamond matrix, Sandia researchers have demonstrated for the first time on a single chip all the components needed to create a quantum bridge to link quantum computers together.

"People have already built small quantum computers," says Sandia researcher Ryan Camacho. "Maybe the first useful one won't be a single giant quantum computer...

Im Focus: New Products - Highlights of COMPAMED 2016

COMPAMED has become the leading international marketplace for suppliers of medical manufacturing. The trade fair, which takes place every November and is co-located to MEDICA in Dusseldorf, has been steadily growing over the past years and shows that medical technology remains a rapidly growing market.

In 2016, the joint pavilion by the IVAM Microtechnology Network, the Product Market “High-tech for Medical Devices”, will be located in Hall 8a again and will...

Im Focus: Ultra-thin ferroelectric material for next-generation electronics

'Ferroelectric' materials can switch between different states of electrical polarization in response to an external electric field. This flexibility means they show promise for many applications, for example in electronic devices and computer memory. Current ferroelectric materials are highly valued for their thermal and chemical stability and rapid electro-mechanical responses, but creating a material that is scalable down to the tiny sizes needed for technologies like silicon-based semiconductors (Si-based CMOS) has proven challenging.

Now, Hiroshi Funakubo and co-workers at the Tokyo Institute of Technology, in collaboration with researchers across Japan, have conducted experiments to...

All Focus news of the innovation-report >>>



Event News

#IC2S2: When Social Science meets Computer Science - GESIS will host the IC2S2 conference 2017

14.10.2016 | Event News

Agricultural Trade Developments and Potentials in Central Asia and the South Caucasus

14.10.2016 | Event News

World Health Summit – Day Three: A Call to Action

12.10.2016 | Event News

Latest News

Resolving the mystery of preeclampsia

21.10.2016 | Health and Medicine

Stanford researchers create new special-purpose computer that may someday save us billions

21.10.2016 | Information Technology

From ancient fossils to future cars

21.10.2016 | Materials Sciences

More VideoLinks >>>