Forum for Science, Industry and Business

Sponsored by:     3M 
Search our Site:

 

Fish Blood Preserves Sperm

05.06.2002


In the Arctic and Antarctic seas the water gets cold to minus 1.9 C in winter, but somehow some fish live there. These cold-blooded creatures survive in the icy water because the blood in their veins contains antifreeze proteins and glycoproteins. High levels of the antifreeze proteins are found in the blood serum, they are present in cell cytoplasm and all body fluids except urine. Due to their structure, molecules of antifreeze glycoproteins (AFGP) prevent growth of ice crystals. Natural antifreezes draw researchers` attention as prospective stabilizers for cryobiology. Scientists from the Institute of Cell Biophysics of Russian Academy of Sciences and from the Institute of Fishery have shown that AFGP help to preserve sperm cells at low temperature.



Biologists have often need to save sperm, tissue samples or cell cultures. The preparations are kept in liquid nitrogen at a temperature of -196 degrees C. To prevent their death, the cells are put into a special cryopreserving medium, which is rather toxic. Russian scientists added AFGP to the media and it became possible to half the amount of toxic compounds. The frozen in such way sperm retained its vitality after thawing and at the right amount of antifreeze the spermatozoid activity even increases.

Liquid nitrogen suits well for long-term storage, but in some cases it is not convenient. After few days nitrogen evaporates and needs replenishment. You also have to warm up the preparations in a special way so that came to life. Not all cells stand very low temperatures, for example, donor organs are not put into liquid nitrogen and they do not have to be kept for several months. If a material should be preserved for several days, it is put into the refrigerator, where the temperature is 4 C. Still, many cells die even in these conditions. The scientists have found that AGFP help in these cases too. They have experimented with sperm of middle-Russian carp. The antifreeze was extracted from the blood serum of cod Gadus morrhua, which lives in the Barents Sea. Usually only 60% of sperm survive after one hour storage in the fridge and 5% after 5 day storage. Adding antifreezes resulted in 2.5 to 6 times increase in the cell activity (the result depended upon the time of storage and the solution composition). AGFP are active in concentration of 2-10 mg/ml.


The researchers suppose that antifreezes interact with cell membrane and do not let it destruct because of freezing. Antifreezes are a mixture of glycoproteins. They can be separated into fractions, but the fractions are ineffective when used alone. Only adding them in their natural proportions protects from freezing. Why? The answer is to be found in further studies. The scientists believe that their discovery is perspective for researches in genome cryopreservation, low temperature storage of reproductive cells of farm animals and also in medicine researches - to provide organs and tissues preservation.

Natalia Reznik | alphagalileo

More articles from Life Sciences:

nachricht Nanoparticle Exposure Can Awaken Dormant Viruses in the Lungs
16.01.2017 | Helmholtz Zentrum München - Deutsches Forschungszentrum für Gesundheit und Umwelt

nachricht Cholera bacteria infect more effectively with a simple twist of shape
13.01.2017 | Princeton University

All articles from Life Sciences >>>

The most recent press releases about innovation >>>

Die letzten 5 Focus-News des innovations-reports im Überblick:

Im Focus: Interfacial Superconductivity: Magnetic and superconducting order revealed simultaneously

Researchers from the University of Hamburg in Germany, in collaboration with colleagues from the University of Aarhus in Denmark, have synthesized a new superconducting material by growing a few layers of an antiferromagnetic transition-metal chalcogenide on a bismuth-based topological insulator, both being non-superconducting materials.

While superconductivity and magnetism are generally believed to be mutually exclusive, surprisingly, in this new material, superconducting correlations...

Im Focus: Studying fundamental particles in materials

Laser-driving of semimetals allows creating novel quasiparticle states within condensed matter systems and switching between different states on ultrafast time scales

Studying properties of fundamental particles in condensed matter systems is a promising approach to quantum field theory. Quasiparticles offer the opportunity...

Im Focus: Designing Architecture with Solar Building Envelopes

Among the general public, solar thermal energy is currently associated with dark blue, rectangular collectors on building roofs. Technologies are needed for aesthetically high quality architecture which offer the architect more room for manoeuvre when it comes to low- and plus-energy buildings. With the “ArKol” project, researchers at Fraunhofer ISE together with partners are currently developing two façade collectors for solar thermal energy generation, which permit a high degree of design flexibility: a strip collector for opaque façade sections and a solar thermal blind for transparent sections. The current state of the two developments will be presented at the BAU 2017 trade fair.

As part of the “ArKol – development of architecturally highly integrated façade collectors with heat pipes” project, Fraunhofer ISE together with its partners...

Im Focus: How to inflate a hardened concrete shell with a weight of 80 t

At TU Wien, an alternative for resource intensive formwork for the construction of concrete domes was developed. It is now used in a test dome for the Austrian Federal Railways Infrastructure (ÖBB Infrastruktur).

Concrete shells are efficient structures, but not very resource efficient. The formwork for the construction of concrete domes alone requires a high amount of...

Im Focus: Bacterial Pac Man molecule snaps at sugar

Many pathogens use certain sugar compounds from their host to help conceal themselves against the immune system. Scientists at the University of Bonn have now, in cooperation with researchers at the University of York in the United Kingdom, analyzed the dynamics of a bacterial molecule that is involved in this process. They demonstrate that the protein grabs onto the sugar molecule with a Pac Man-like chewing motion and holds it until it can be used. Their results could help design therapeutics that could make the protein poorer at grabbing and holding and hence compromise the pathogen in the host. The study has now been published in “Biophysical Journal”.

The cells of the mouth, nose and intestinal mucosa produce large quantities of a chemical called sialic acid. Many bacteria possess a special transport system...

All Focus news of the innovation-report >>>

Anzeige

Anzeige

Event News

12V, 48V, high-voltage – trends in E/E automotive architecture

10.01.2017 | Event News

2nd Conference on Non-Textual Information on 10 and 11 May 2017 in Hannover

09.01.2017 | Event News

Nothing will happen without batteries making it happen!

05.01.2017 | Event News

 
Latest News

Water - as the underlying driver of the Earth’s carbon cycle

17.01.2017 | Earth Sciences

Interfacial Superconductivity: Magnetic and superconducting order revealed simultaneously

17.01.2017 | Materials Sciences

Smart homes will “LISTEN” to your voice

17.01.2017 | Architecture and Construction

VideoLinks
B2B-VideoLinks
More VideoLinks >>>