Forum for Science, Industry and Business

Sponsored by:     3M 
Search our Site:

 

Fish Blood Preserves Sperm

05.06.2002


In the Arctic and Antarctic seas the water gets cold to minus 1.9 C in winter, but somehow some fish live there. These cold-blooded creatures survive in the icy water because the blood in their veins contains antifreeze proteins and glycoproteins. High levels of the antifreeze proteins are found in the blood serum, they are present in cell cytoplasm and all body fluids except urine. Due to their structure, molecules of antifreeze glycoproteins (AFGP) prevent growth of ice crystals. Natural antifreezes draw researchers` attention as prospective stabilizers for cryobiology. Scientists from the Institute of Cell Biophysics of Russian Academy of Sciences and from the Institute of Fishery have shown that AFGP help to preserve sperm cells at low temperature.



Biologists have often need to save sperm, tissue samples or cell cultures. The preparations are kept in liquid nitrogen at a temperature of -196 degrees C. To prevent their death, the cells are put into a special cryopreserving medium, which is rather toxic. Russian scientists added AFGP to the media and it became possible to half the amount of toxic compounds. The frozen in such way sperm retained its vitality after thawing and at the right amount of antifreeze the spermatozoid activity even increases.

Liquid nitrogen suits well for long-term storage, but in some cases it is not convenient. After few days nitrogen evaporates and needs replenishment. You also have to warm up the preparations in a special way so that came to life. Not all cells stand very low temperatures, for example, donor organs are not put into liquid nitrogen and they do not have to be kept for several months. If a material should be preserved for several days, it is put into the refrigerator, where the temperature is 4 C. Still, many cells die even in these conditions. The scientists have found that AGFP help in these cases too. They have experimented with sperm of middle-Russian carp. The antifreeze was extracted from the blood serum of cod Gadus morrhua, which lives in the Barents Sea. Usually only 60% of sperm survive after one hour storage in the fridge and 5% after 5 day storage. Adding antifreezes resulted in 2.5 to 6 times increase in the cell activity (the result depended upon the time of storage and the solution composition). AGFP are active in concentration of 2-10 mg/ml.


The researchers suppose that antifreezes interact with cell membrane and do not let it destruct because of freezing. Antifreezes are a mixture of glycoproteins. They can be separated into fractions, but the fractions are ineffective when used alone. Only adding them in their natural proportions protects from freezing. Why? The answer is to be found in further studies. The scientists believe that their discovery is perspective for researches in genome cryopreservation, low temperature storage of reproductive cells of farm animals and also in medicine researches - to provide organs and tissues preservation.

Natalia Reznik | alphagalileo

More articles from Life Sciences:

nachricht The birth of a new protein
20.10.2017 | University of Arizona

nachricht Building New Moss Factories
20.10.2017 | Albert-Ludwigs-Universität Freiburg im Breisgau

All articles from Life Sciences >>>

The most recent press releases about innovation >>>

Die letzten 5 Focus-News des innovations-reports im Überblick:

Im Focus: Neutron star merger directly observed for the first time

University of Maryland researchers contribute to historic detection of gravitational waves and light created by event

On August 17, 2017, at 12:41:04 UTC, scientists made the first direct observation of a merger between two neutron stars--the dense, collapsed cores that remain...

Im Focus: Breaking: the first light from two neutron stars merging

Seven new papers describe the first-ever detection of light from a gravitational wave source. The event, caused by two neutron stars colliding and merging together, was dubbed GW170817 because it sent ripples through space-time that reached Earth on 2017 August 17. Around the world, hundreds of excited astronomers mobilized quickly and were able to observe the event using numerous telescopes, providing a wealth of new data.

Previous detections of gravitational waves have all involved the merger of two black holes, a feat that won the 2017 Nobel Prize in Physics earlier this month....

Im Focus: Smart sensors for efficient processes

Material defects in end products can quickly result in failures in many areas of industry, and have a massive impact on the safe use of their products. This is why, in the field of quality assurance, intelligent, nondestructive sensor systems play a key role. They allow testing components and parts in a rapid and cost-efficient manner without destroying the actual product or changing its surface. Experts from the Fraunhofer IZFP in Saarbrücken will be presenting two exhibits at the Blechexpo in Stuttgart from 7–10 November 2017 that allow fast, reliable, and automated characterization of materials and detection of defects (Hall 5, Booth 5306).

When quality testing uses time-consuming destructive test methods, it can result in enormous costs due to damaging or destroying the products. And given that...

Im Focus: Cold molecules on collision course

Using a new cooling technique MPQ scientists succeed at observing collisions in a dense beam of cold and slow dipolar molecules.

How do chemical reactions proceed at extremely low temperatures? The answer requires the investigation of molecular samples that are cold, dense, and slow at...

Im Focus: Shrinking the proton again!

Scientists from the Max Planck Institute of Quantum Optics, using high precision laser spectroscopy of atomic hydrogen, confirm the surprisingly small value of the proton radius determined from muonic hydrogen.

It was one of the breakthroughs of the year 2010: Laser spectroscopy of muonic hydrogen resulted in a value for the proton charge radius that was significantly...

All Focus news of the innovation-report >>>

Anzeige

Anzeige

Event News

ASEAN Member States discuss the future role of renewable energy

17.10.2017 | Event News

World Health Summit 2017: International experts set the course for the future of Global Health

10.10.2017 | Event News

Climate Engineering Conference 2017 Opens in Berlin

10.10.2017 | Event News

 
Latest News

Terahertz spectroscopy goes nano

20.10.2017 | Information Technology

Strange but true: Turning a material upside down can sometimes make it softer

20.10.2017 | Materials Sciences

NRL clarifies valley polarization for electronic and optoelectronic technologies

20.10.2017 | Interdisciplinary Research

VideoLinks
B2B-VideoLinks
More VideoLinks >>>