Forum for Science, Industry and Business

Sponsored by:     3M 
Search our Site:

 

Cells Get Sprayed

17.01.2008
Water droplets produced by electrospray render cells permeable to external DNA

Genetically engineered products have become indispensable.

For example, genetically modified bacteria produce human insulin. In future, gene therapy should make it possible to introduce genes into the cells of a diseased organism so that they can address deficiencies to compensate for malfunctions in the body. In order for this to work, foreign (or synthetic) DNA must be introduced into host cells, which is not exactly a trivial task.

Japanese researchers have now developed a method which could represent a true alternative to conventional processes. As described in the journal Angewandte Chemie, the cells are “bombarded” with water droplets produced and accelerated by electrospray.

... more about:
»DNA »droplets »method

There are several methods to transfer DNA into a host cell. In the simplest case the foreign DNA forces its way into the cell through a cell membrane that has been made porous, through treatment with electrical current or UV lasers, for example. Viruses and liposomes can be used as genetic transporters and the genetic material can be injected or shot into the cell with a “particle gun”. These methods all have the disadvantage of either severely damaging delicate cells or of being markedly expensive and complicated.

A team at the Saitama University led by Takafumi Sakai, in cooperation with Kazuto Ikemoto (Mitsubishi Gas Chemical Company), has now developed a methodology that could provide an alternative: They “bombard” the cells with tiny electrically charged water droplets. The droplets tear tiny holes in the cell membranes, through which external DNA molecules can enter. After about one minute, the holes have closed back up and even delicate cells survive the procedure undamaged.

This method is based on a technique called electrospray, which has long been used with success, particularly in mass spectrometry. In this process, the tip of an extremely fine steel capillary is put under a high voltage. A highly charged drop of water exits the capillary and is atomized into many micro- or nanoscopic droplets. These charged microdroplets are strongly accelerated in an electrical field—toward the plate holding the cell culture.

The advantage of this new method: It is suitable for a large variety of cell types—mammalian cell cultures and bacteria, as well as living tissue, as was demonstrated with bird embryos. No cytotoxic reagents that could damage the cells are needed; only pure water or a cell-tolerated saline solution are used. An entire plate of cell cultures can be “sprayed” bit by bit, or a specific point on some tissue can be targeted. The equipment needed is simple, inexpensive, and portable.

Author: Takafumi Sakai, Saitama University (Japan), mailto:tsakai@mail.saitama-u.ac.jp

Title: DNA Introduction into Living Cells by Water Droplet Impact with an Electrospray Process

Angewandte Chemie International Edition, doi: 10.1002/anie.200704429

Takafumi Sakai | Angewandte Chemie
Further information:
http://pressroom.angewandte.org

Further reports about: DNA droplets method

More articles from Life Sciences:

nachricht Water forms 'spine of hydration' around DNA, group finds
26.05.2017 | Cornell University

nachricht How herpesviruses win the footrace against the immune system
26.05.2017 | Helmholtz-Zentrum für Infektionsforschung

All articles from Life Sciences >>>

The most recent press releases about innovation >>>

Die letzten 5 Focus-News des innovations-reports im Überblick:

Im Focus: Can the immune system be boosted against Staphylococcus aureus by delivery of messenger RNA?

Staphylococcus aureus is a feared pathogen (MRSA, multi-resistant S. aureus) due to frequent resistances against many antibiotics, especially in hospital infections. Researchers at the Paul-Ehrlich-Institut have identified immunological processes that prevent a successful immune response directed against the pathogenic agent. The delivery of bacterial proteins with RNA adjuvant or messenger RNA (mRNA) into immune cells allows the re-direction of the immune response towards an active defense against S. aureus. This could be of significant importance for the development of an effective vaccine. PLOS Pathogens has published these research results online on 25 May 2017.

Staphylococcus aureus (S. aureus) is a bacterium that colonizes by far more than half of the skin and the mucosa of adults, usually without causing infections....

Im Focus: A quantum walk of photons

Physicists from the University of Würzburg are capable of generating identical looking single light particles at the push of a button. Two new studies now demonstrate the potential this method holds.

The quantum computer has fuelled the imagination of scientists for decades: It is based on fundamentally different phenomena than a conventional computer....

Im Focus: Turmoil in sluggish electrons’ existence

An international team of physicists has monitored the scattering behaviour of electrons in a non-conducting material in real-time. Their insights could be beneficial for radiotherapy.

We can refer to electrons in non-conducting materials as ‘sluggish’. Typically, they remain fixed in a location, deep inside an atomic composite. It is hence...

Im Focus: Wafer-thin Magnetic Materials Developed for Future Quantum Technologies

Two-dimensional magnetic structures are regarded as a promising material for new types of data storage, since the magnetic properties of individual molecular building blocks can be investigated and modified. For the first time, researchers have now produced a wafer-thin ferrimagnet, in which molecules with different magnetic centers arrange themselves on a gold surface to form a checkerboard pattern. Scientists at the Swiss Nanoscience Institute at the University of Basel and the Paul Scherrer Institute published their findings in the journal Nature Communications.

Ferrimagnets are composed of two centers which are magnetized at different strengths and point in opposing directions. Two-dimensional, quasi-flat ferrimagnets...

Im Focus: World's thinnest hologram paves path to new 3-D world

Nano-hologram paves way for integration of 3-D holography into everyday electronics

An Australian-Chinese research team has created the world's thinnest hologram, paving the way towards the integration of 3D holography into everyday...

All Focus news of the innovation-report >>>

Anzeige

Anzeige

Event News

Marine Conservation: IASS Contributes to UN Ocean Conference in New York on 5-9 June

24.05.2017 | Event News

AWK Aachen Machine Tool Colloquium 2017: Internet of Production for Agile Enterprises

23.05.2017 | Event News

Dortmund MST Conference presents Individualized Healthcare Solutions with micro and nanotechnology

22.05.2017 | Event News

 
Latest News

How herpesviruses win the footrace against the immune system

26.05.2017 | Life Sciences

Water forms 'spine of hydration' around DNA, group finds

26.05.2017 | Life Sciences

First Juno science results supported by University of Leicester's Jupiter 'forecast'

26.05.2017 | Physics and Astronomy

VideoLinks
B2B-VideoLinks
More VideoLinks >>>