Researchers at the Massachusetts General Hospital (MGH)/Harvard Medical School, Drs. Beste Kinikoglu and Yawei Kong, led by Dr. Eric C. Liao, cultured and characterized for the first time multipotent neural crest cells isolated from zebrafish embryos.
This important study is reported in the February 2014 issue of Experimental Biology and Medicine. Neural crest is a unique cell population induced at the lateral border of the neural plate during embryogenesis and vertebrate development depends on these multipotent migratory cells.
Defects in neural crest development result in a wide range of malformations, such as cleft lip and palate, and diseases, such as melanoma. Dr. Liao's laboratory uses zebrafish as a model vertebrate to study the genetic basis of neural crest related craniofacial malformations. Zebrafish has long been used to study early development and recently emerged as a model to study disease.
"Development of in vitro culture of neural crest cells and reproducible functional assays will provide a valuable and complementary approach to the in vivo experiments in zebrafish" said Dr. Eric C. Liao, senior author of the study and an Assistant Professor of Surgery at MGH, and Principal Faculty at the Harvard Stem Cell Institute.
The team took advantage of the sox 10 reporter transgenic model to enrich and isolate the neural crest cells (NCCs), which were subsequently cultured under optimized culture conditions. Cultured NCCs were found to express major neural crest lineage markers such as sox10, sox9a, hnk1, p75, dlx2a, and pax3, and the pluripotency markers c-myc and klf4.
The cells could be further differentiated into multiple neural crest lineages, contributing to neurons, glial cells, smooth muscle cells, melanocytes, and chondrocytes. Using the functional cell behavior assays that they developed, the team was able to assess the influence of retinoic acid, an endogenously synthesized, powerful, morphogenetic molecule, on NCC behavior.
This study showed that retinoic acid had a profound effect on NCC morphology and differentiation, significantly inhibited proliferation and enhanced cell migration. The data implicate NCCs as a target cell population for retinoic acid and suggest that it plays multiple critical roles in NCC development.
"We hope that our novel neural crest system will be useful to gain mechanistic understanding of NCC development and for cell-based high-throughput drug screening applications" said Dr. Beste Kinikoglu, a postdoctoral fellow in Dr. Liao's laboratory and the study's first author. Dr. Steven R. Goodman, Editor-in-Chief of Experimental Biology and Medicine said "Liao and colleagues have provided the first zebrafish embryo derived NCC pure population in vitro model for the study of neural crest development. I believe that this will be a valuable tool for this purpose".
Experimental Biology and Medicine is a journal dedicated to the publication of multidisciplinary and interdisciplinary research in the biomedical sciences. The journal was first established in 1903. Experimental Biology and Medicine is the journal of the Society of Experimental Biology and Medicine. To learn about the benefits of society membership visit http://www.sebm.org. If you are interested in publishing in the journal please visit http://ebm.sagepub.com/.
Eric C. Liao | EurekAlert!
Locusts at the wheel: University of Graz investigates collision detector inspired by insect eyes
07.10.2015 | Karl-Franzens-Universität Graz
Flipping molecular attachments amps up activity of CO2 catalyst
06.10.2015 | DOE/Brookhaven National Laboratory
Self-driving cars will be on our streets in the foreseeable future. In Graz, research is currently dedicated to an innovative driver assistance system that takes over control if there is a danger of collision. It was nature that inspired Dr Manfred Hartbauer from the Institute of Zoology at the University of Graz: in dangerous traffic situations, migratory locusts react around ten times faster than humans. Working together with an interdisciplinary team, Hartbauer is investigating an affordable collision detector that is equipped with artificial locust eyes and can recognise potential crashes in time, during both day and night.
Inspired by insects
An interdisciplinary team of researchers has built the first prototype of a miniature particle accelerator that uses terahertz radiation instead of radio...
At present, tiny magnetic whirls – so called skyrmions – are discussed as promising candidates for bits in future robust and compact data storage devices. At...
In cooperation with the Center for Nano-Optics of Georgia State University in Atlanta (USA), scientists of the Laboratory for Attosecond Physics of the Max Planck Institute of Quantum Optics and the Ludwig-Maximilians-Universität have made simulations of the processes that happen when a layer of carbon atoms is irradiated with strong laser light.
Electrons hit by strong laser pulses change their location on ultrashort timescales, i.e. within a couple of attoseconds (1 as = 10 to the minus 18 sec). In...
At the exhibition BATTERY + STORAGE as part of WORLD OF ENERGY SOLUTIONS 2015 in Stuttgart, the Fraunhofer Institutes for Laser Technology ILT and for Ceramic Technologies and Systems IKTS will be showing how laser technology can be used to manufacture batteries both cost- and energy-efficiently.
In the truest sense, it’s all about watts at the Dresden-based Fraunhofer Institute for Ceramic Technologies and Systems IKTS and the Aachen-based Fraunhofer...
01.10.2015 | Event News
30.09.2015 | Event News
17.09.2015 | Event News
07.10.2015 | Life Sciences
07.10.2015 | Machine Engineering
06.10.2015 | Information Technology