"Cell movement is the basic recipe of life, and all cells have the capacity to move," says Roberto Dominguez, PhD, professor of Physiology at the Perelman School of Medicine, University of Pennsylvania.
This image shows the active, open state of IRSp53 (top) and inactive, closed (bottom) state of IRSp53. In the closed state, cells do not generate filopodia as shown in the right bottom image (green=IRSp53, red=cdc42, and blue=actin, the most abundant protein in the cytoskeleton). The arrow between the two states indicates that the synergistic binding of Cdc42, cytoskeleton proteins (called downstream effectors of IRSp53 and includes the tumor-promoting factor Eps8) and the inherent attraction for the cell membrane, bring IRSp53 to specific locations on the cell membrane in which to change the shape of the cell. Chief among this reshaping activity is generating filopodia, the long thin objects coming off the cell in the top right image (color scheme as right bottom image). Note the change in pattern of the green and red show that IRSp53 and cdc42 are working together and moving to many different locations around the cell.
Credit: Roberto Dominguez, Ph.D., David Kast, Ph.D., Perelman School of Medicine, University of Pennsylvania
Motility – albeit on a cellular spatial scale -- is necessary for wound healing, clotting, fetal development, nerve connections, and the immune response, among other functions. On the other hand, cell movement can be deleterious when cancer cells break away from tumors and migrate to set up shop in other tissues during cancer metastasis.
The Dominguez team, with postdoctoral fellow David Kast, PhD, and colleagues, report online ahead of print in Nature Structural & Molecular Biology how a key cell-movement protein called IRSp53 is regulated in a resting and active state, and what this means for cancer-cell metastasis.
"We characterized how IRSp53 connects to the cell-motility machinery," says Kast. "It does this by starting the formation of cell filopodia - extensions that form when a cell needs to move."
"Cells move like an inchworm," explains Dominguez. "Filopodia are at the leading edge of moving cells." The trailing end of the cell follows the move forward through contraction of actin and myosin in the cytoskeleton, much like muscle contraction. A cell pushes out the leading edge of its membrane, and sticks it down on whatever it is moving across, namely other cells, and then moves the cell body along, unsticking the back end. This sets the cell up for its next move.
IRSp53 contains a region called a BAR domain that binds to and shapes cell membranes. Other parts of the protein connect it to the cytoskeleton (internal bits that give a cell structure and shape). Together, through the binding of cell membranes and other proteins IRSp53 regulates cell movement. The team found that in the resting state, human IRSp53 adopts a closed shape that prevents it from interacting with the membrane and the cytoskeleton. However, the binding of a signaling protein, called Cdc42, opens IRSp53, setting in motion the recruitment of a complex cellular machinery needed for motility.
One of the cytoskeleton components IRSp53 connects to is the tumor-promoting protein Eps8. IRSp53 is synergistically activated by the combined action of Cdc42 and binding of Eps8, which is upregulated in metastatic cancers.
Co-authors Tatyana Svitkina and Changsong Yang from the Penn Department of Biology, brought their expertise with living cells to the study. By introducing normal and mutant proteins into cells they could see how these proteins induced filopodia to form. The team found that mutations in critical regions of IRSp53 can either lead to enhanced or reduced filopodia formation and, as a consequence, cell motility. "This finding shows how all these different proteins converge on IRSp53 to execute precise cellular functions, and that when one factor is disrupted, other proteins are affected down the activity pathway," says Dominguez.
The team's next steps will be to screen libraries of small molecule inhibitors that interfere with the IRSp53-Eps8 interaction, to figure out how to stop unwanted cell movement before it gets too far.
Coauthors include Yadaiah Madasu and Malgorzata Boczkowska, also from Physiology, and Andrea Disanza and Giorgio Scita from the Institute of Molecular Oncology and the University of Milan School of Medicine in Italy.
The research was funded by the National Institutes of Health (R01 MH087950, T32 AR053461, GM095977) and the American Cancer Society (PF-13-033-01-DMC).
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