Forum for Science, Industry and Business

Sponsored by:     3M 
Search our Site:

 

No need for water, enzymes are doing it for themselves

06.10.2014

New research by scientists at the University of Bristol has challenged one of the key axioms in biology - that enzymes need water to function. The breakthrough could eventually lead to the development of new industrial catalysts for processing biodiesel.

Enzymes are large biological molecules that catalyse thousands of different chemical reactions that are essential for all life, from converting food into energy, to controlling how our cells replicate DNA.


Optical microscopy images showing a mixture of the liquid enzyme (yellow material) with the solid substrate (black crystals) immediately after contact (left), and after incubation for 30 min at 50°C (right). The development of the yellow colouration arises from the lipase-catalysed formation the yellow product.

Credit: University of Bristol

Throughout this diverse range of biological environments in which enzymes perform their various roles, the only constant is an abundance of water.

However, new findings published today [6 October] in Nature Communications, show that water is not essential for enzymes to fulfil their biological role.

This discovery could pave the way for the development of new thermally robust industrial enzymes that could be utilised in harsh processing conditions, with applications ranging from detergent technologies to alternative energies via biofuel production.

Dr Adam Perriman and colleagues were able to circumvent the need for water by decorating the surface of the industrial enzyme lipase with long detergent molecules.

In principle, what the team created was an enzyme with an in-built ability to exist as a liquid without any solvent. What was astounding was that the solid chemical reactant, also known as the substrate, could be dissolved directly by the liquid enzyme, which then went on to catalyse the chemical reaction, and would continue to do so up to temperatures as high as 150° C.

Dr Perriman, from Bristol University's School of Cellular and Molecular Medicine, said: "From our preliminary experiments, we knew that the molecular structure of the lipase was still intact after the modifications, even at 150° C.

"However, we were surprised and delighted to discover that the catalytic activity of the enzyme was still present. The ability to rationally design a self-contained reactive biofluid, where one can literally sprinkle a solid substrate onto it, and then observe a chemical reaction, represents a real fundamental scientific advance."

###

'Enzyme activity in liquid lipase melts as a step towards solvent-free biology at 150°C' by Alex Brogan, Kamendra Sharma, Adam Perriman and Stephen Mann in Nature Communications.

Philippa Walker | Eurek Alert!
Further information:
http://www.bristol.ac.uk

More articles from Life Sciences:

nachricht Topologische Quantenchemie
21.07.2017 | Max-Planck-Institut für Chemische Physik fester Stoffe

nachricht Topological Quantum Chemistry
21.07.2017 | Max-Planck-Institut für Chemische Physik fester Stoffe

All articles from Life Sciences >>>

The most recent press releases about innovation >>>

Die letzten 5 Focus-News des innovations-reports im Überblick:

Im Focus: Manipulating Electron Spins Without Loss of Information

Physicists have developed a new technique that uses electrical voltages to control the electron spin on a chip. The newly-developed method provides protection from spin decay, meaning that the contained information can be maintained and transmitted over comparatively large distances, as has been demonstrated by a team from the University of Basel’s Department of Physics and the Swiss Nanoscience Institute. The results have been published in Physical Review X.

For several years, researchers have been trying to use the spin of an electron to store and transmit information. The spin of each electron is always coupled...

Im Focus: The proton precisely weighted

What is the mass of a proton? Scientists from Germany and Japan successfully did an important step towards the most exact knowledge of this fundamental constant. By means of precision measurements on a single proton, they could improve the precision by a factor of three and also correct the existing value.

To determine the mass of a single proton still more accurate – a group of physicists led by Klaus Blaum and Sven Sturm of the Max Planck Institute for Nuclear...

Im Focus: On the way to a biological alternative

A bacterial enzyme enables reactions that open up alternatives to key industrial chemical processes

The research team of Prof. Dr. Oliver Einsle at the University of Freiburg's Institute of Biochemistry has long been exploring the functioning of nitrogenase....

Im Focus: The 1 trillion tonne iceberg

Larsen C Ice Shelf rift finally breaks through

A one trillion tonne iceberg - one of the biggest ever recorded -- has calved away from the Larsen C Ice Shelf in Antarctica, after a rift in the ice,...

Im Focus: Laser-cooled ions contribute to better understanding of friction

Physics supports biology: Researchers from PTB have developed a model system to investigate friction phenomena with atomic precision

Friction: what you want from car brakes, otherwise rather a nuisance. In any case, it is useful to know as precisely as possible how friction phenomena arise –...

All Focus news of the innovation-report >>>

Anzeige

Anzeige

Event News

Closing the Sustainability Circle: Protection of Food with Biobased Materials

21.07.2017 | Event News

»We are bringing Additive Manufacturing to SMEs«

19.07.2017 | Event News

The technology with a feel for feelings

12.07.2017 | Event News

 
Latest News

NASA looks to solar eclipse to help understand Earth's energy system

21.07.2017 | Earth Sciences

Stanford researchers develop a new type of soft, growing robot

21.07.2017 | Power and Electrical Engineering

Vortex photons from electrons in circular motion

21.07.2017 | Physics and Astronomy

VideoLinks
B2B-VideoLinks
More VideoLinks >>>