Forum for Science, Industry and Business

Sponsored by:     3M 
Search our Site:

 

Mouse pancreatic stem cells successfully differentiate into insulin producing cells

26.09.2012
In a study to investigate how transplanted islet cells can differentiate and mature into insulin-producing pancreatic cells, a team of Japanese researchers found that using a specific set of transcription factors (proteins that bind to specific DNA sequences) could be transduced into mouse pancreatic stem cells (mPSCs) using Sendai virus (SeV), a mouse influenza virus, as a carrier, or vector. The study is published in a recent issue of Cell Medicine [3(1)], now freely available on-line at: http://www.ingentaconnect.com/content/cog/cm.

"Diabetes is one of the most serious and prevalent metabolic diseases," said study co-author Dr. Hiroshi Yukawa, Department of Advanced Medicine in Biotechnology and Robotics, Nagoya University Graduate School of Medicine. "Islet cell transplantation has proven effective, however this strategy requires sufficient organ donors."

Given the shortage of donors, the researchers investigated factors that could impact on the expansion and differentiation of pancreatic stem cells (PSCs) into insulin-producing cells using combinations of varieties of transcription factors and the SeV mouse virus to carry the cells, thus increasing the number of functional islet cells available for transplantation.

SeV vectors, said the researchers, are superior to conventional virus vectors because "they do not go through a DNA phase" and can introduce foreign genes without toxicity into a variety of cell types.

The combination of transcription factors that produced the greatest impact on the differentiation of PSCs into insulin cells was Pdx-1 (Pancreatic and duodenal homeobox 1), NeuroD (neurogenic differentiation) and MafA (musculoaponeurotic fibrosarcoma oncogene A). "Our data suggest that the transduction of transcription factors using SeV vectors facilitates mPSCs differentiation into insulin producing cells and showed the possibility of regenerating B-cells by using transduced PSCs," concluded the researchers.

This research was among those studies presented at the 37th Annual Meeting of the Japan Society for Organ Preservation and Medical Biology (JSOPMB). Sixteen studies were published in this special issue of CELL MEDICINE. The theme of the issue is "Organ/Cell Transplantation and Regenerative Medicine."

Citation. Yukawa, H.; Noguchi, H.; Oishi, K.; Miyamoto, Y.; Inoue, M.; Hasegawa, M.; Hayashi, S. Differentiation of Mouse Pancreatic Stem Cells into Insulin-Producing Cells by Recombinant Sendai Virus-Mediated Gene Transfer Technology Cell Med. 3(1):51-61; 2012.
Contact: Hiroshi Yukawa, Immuno-therapy Research Group, Fundamental Research Department, Central R&D Laboratory, Kobayashi Pharmaceutical Co., LTD
Tel: ++ 81-72-640-0114; Fax ++81-72-640-0118
Email: h.yukawa@kobayyashi.cp.jp
The editorial offices for CELL MEDICINE are at the Center of Excellence for Aging and Brain Repair, College of Medicine, the University of South Florida. Contact, David Eve, PhD. at cellmedicinect@gmail.com

News Release by Florida Science Communications.

David Eve | EurekAlert!
Further information:
http://www.ingentaconnect.com/content/cog/cm

More articles from Life Sciences:

nachricht A Map of the Cell’s Power Station
18.08.2017 | Albert-Ludwigs-Universität Freiburg im Breisgau

nachricht On the way to developing a new active ingredient against chronic infections
18.08.2017 | Deutsches Zentrum für Infektionsforschung

All articles from Life Sciences >>>

The most recent press releases about innovation >>>

Die letzten 5 Focus-News des innovations-reports im Überblick:

Im Focus: Fizzy soda water could be key to clean manufacture of flat wonder material: Graphene

Whether you call it effervescent, fizzy, or sparkling, carbonated water is making a comeback as a beverage. Aside from quenching thirst, researchers at the University of Illinois at Urbana-Champaign have discovered a new use for these "bubbly" concoctions that will have major impact on the manufacturer of the world's thinnest, flattest, and one most useful materials -- graphene.

As graphene's popularity grows as an advanced "wonder" material, the speed and quality at which it can be manufactured will be paramount. With that in mind,...

Im Focus: Exotic quantum states made from light: Physicists create optical “wells” for a super-photon

Physicists at the University of Bonn have managed to create optical hollows and more complex patterns into which the light of a Bose-Einstein condensate flows. The creation of such highly low-loss structures for light is a prerequisite for complex light circuits, such as for quantum information processing for a new generation of computers. The researchers are now presenting their results in the journal Nature Photonics.

Light particles (photons) occur as tiny, indivisible portions. Many thousands of these light portions can be merged to form a single super-photon if they are...

Im Focus: Circular RNA linked to brain function

For the first time, scientists have shown that circular RNA is linked to brain function. When a RNA molecule called Cdr1as was deleted from the genome of mice, the animals had problems filtering out unnecessary information – like patients suffering from neuropsychiatric disorders.

While hundreds of circular RNAs (circRNAs) are abundant in mammalian brains, one big question has remained unanswered: What are they actually good for? In the...

Im Focus: RAVAN CubeSat measures Earth's outgoing energy

An experimental small satellite has successfully collected and delivered data on a key measurement for predicting changes in Earth's climate.

The Radiometer Assessment using Vertically Aligned Nanotubes (RAVAN) CubeSat was launched into low-Earth orbit on Nov. 11, 2016, in order to test new...

Im Focus: Scientists shine new light on the “other high temperature superconductor”

A study led by scientists of the Max Planck Institute for the Structure and Dynamics of Matter (MPSD) at the Center for Free-Electron Laser Science in Hamburg presents evidence of the coexistence of superconductivity and “charge-density-waves” in compounds of the poorly-studied family of bismuthates. This observation opens up new perspectives for a deeper understanding of the phenomenon of high-temperature superconductivity, a topic which is at the core of condensed matter research since more than 30 years. The paper by Nicoletti et al has been published in the PNAS.

Since the beginning of the 20th century, superconductivity had been observed in some metals at temperatures only a few degrees above the absolute zero (minus...

All Focus news of the innovation-report >>>

Anzeige

Anzeige

Event News

Call for Papers – ICNFT 2018, 5th International Conference on New Forming Technology

16.08.2017 | Event News

Sustainability is the business model of tomorrow

04.08.2017 | Event News

Clash of Realities 2017: Registration now open. International Conference at TH Köln

26.07.2017 | Event News

 
Latest News

A Map of the Cell’s Power Station

18.08.2017 | Life Sciences

Engineering team images tiny quasicrystals as they form

18.08.2017 | Physics and Astronomy

Researchers printed graphene-like materials with inkjet

18.08.2017 | Materials Sciences

VideoLinks
B2B-VideoLinks
More VideoLinks >>>