Forum for Science, Industry and Business

Sponsored by:     3M 
Search our Site:

 

How a molecular Superman protects the genome from damage

17.10.2014

Scientists find a new role for RNAi protein Dicer in preventing collisions during DNA replication

How many times have we seen Superman swoop down from the heavens and rescue a would-be victim from a rapidly oncoming train?

How Dicer Protects the Yeast Genome from Damage

CSHL scientists have found a new role for the RNAi protein Dicer in preserving genomic stability in yeast. Dicer helps prevent collisions between transcription and replication machinery as the genetic material is copied. In cells that lack Dicer, DNA damage accumulates strikingly (yellow spots in the cells above), leading to DNA rearrangements that are associated with aging and disease.

Credit: Cold Spring Harbor Laboratory/ Jie Ren and Stephen Hearn

It's a familiar scenario, played out hundreds of times in the movies. But the dramatic scene is reenacted in real life every time a cell divides. In order for division to occur, our genetic material must be faithfully replicated by a highly complicated machine, whose parts are tiny enough to navigate among the strands of the double helix.

The problem is that our DNA is constantly in use, with other molecular machines continually plucking at its strands to gain access to critical genes. In this other process, known as transcription, the letters of our DNA are being copied to form a template that will guide the formation of proteins. But these two copying machines can't occupy the same bit of genetic track at once. Inevitably they will collide – unless a molecular Superman can remove the transcription machinery and save the day.

Cold Spring Harbor Laboratory (CSHL) scientists have found that this molecular Superman exists in the form of a protein known as Dicer. Better known for its role in selectively silencing genes via a process called RNA interference (RNAi), Dicer is now understood to help free transcription machinery from DNA so that replication can occur.

The team, led by Robert Martienssen, a CSHL Professor and Howard Hughes Medical Institute Investigator, concludes that this previously unknown function of Dicer is critical to preserve the integrity of the genome in yeast. They point out that collisions between the replication and transcription machinery lead to massive changes across the genome – changes that are associated with aging and diseases like cancer.

Martienssen and his colleagues previously found that RNAi resolves the conflict between transcription and DNA replication in isolated areas of the genome where genes are being silenced. "When Dicer is mutated, replication stalls and DNA in the region becomes damaged," explains Martienssen. "This was a new role for a protein that we thought functioned solely in RNAi."

In work published today in Cell, Martienssen and his team explored if and how Dicer might function more broadly, across the entire genome. The team, including lead authors Stephane Castel, Ph.D., a graduate of the CSHL Watson School of Biological Sciences, and Jie Ren, Ph.D., a postdoctoral researcher, found that Dicer participates in the release of transcription machinery throughout the genome. "Dicer's function isn't restricted to silenced genes," explains Ren. In fact, it controls the release at hundreds of extremely active genes.

"These are genes that are in constant use by the cell – we call many of them 'housekeeping' genes because they are required for basic survival," says Castel. At any given time, transcription machinery can be found near these genes. Without the help of Dicer, this machinery is headed for an almost certain collision when replication occurs.

Are these collisions really so catastrophic for the cell? The team found that the accidents cause massive segments of DNA to be lost with each cell division. "These chromosome rearrangements, known as genomic instability, are involved in aging and cancer," says Ren. Other groups have shown that mutations in Dicer are similarly associated with an increased risk of tumor formation. The team's discovery may help to explain these observations, according to Martienssen. "It may be that Dicer's role in cancer is to protect the genome by preventing collisions between transcription and replication."

This work was supported by the Natural Sciences and Engineering Research Council of Canada, the Spanish Ministerio de Economía y Competitividad, the National Institutes of Health, the Howard Hughes Medical Institute-Gordon and Betty Moore Foundation, as well as a Cashin Scholarship from the Watson School of Biological Sciences and assistance from the Cold Spring Harbor Laboratory Shared Resources, which are funded in part by the Cancer Center Support Grant.

"Dicer Promotes Transcription Termination at Sites of Replication Stress to Maintain Genome Stability" appears online in Cell on October 16, 2014. The authors are: Stephane Castel, Jie Ren, Sonali Bhattacharjee, An-Yun Chang, Mar Sánchez, Alberto Valbuena, Francisco Antequera, and Robert Martienssen. The paper can be obtained online at: http://www.cell.com

About Cold Spring Harbor Laboratory

Founded in 1890, Cold Spring Harbor Laboratory (CSHL) has shaped contemporary biomedical research and education with programs in cancer, neuroscience, plant biology and quantitative biology. CSHL is ranked number one in the world by Thomson Reuters for the impact of its research in molecular biology and genetics. The Laboratory has been home to eight Nobel Prize winners. Today, CSHL's multidisciplinary scientific community is more than 600 researchers and technicians strong and its Meetings & Courses program hosts more than 12,000 scientists from around the world each year to its Long Island campus and its China center. For more information, visit http://www.cshl.edu

Jaclyn Jansen | Eurek Alert!

Further reports about: DNA Laboratory RNAi collisions damage function genes replication transcription

More articles from Life Sciences:

nachricht The birth of a new protein
20.10.2017 | University of Arizona

nachricht Building New Moss Factories
20.10.2017 | Albert-Ludwigs-Universität Freiburg im Breisgau

All articles from Life Sciences >>>

The most recent press releases about innovation >>>

Die letzten 5 Focus-News des innovations-reports im Überblick:

Im Focus: Neutron star merger directly observed for the first time

University of Maryland researchers contribute to historic detection of gravitational waves and light created by event

On August 17, 2017, at 12:41:04 UTC, scientists made the first direct observation of a merger between two neutron stars--the dense, collapsed cores that remain...

Im Focus: Breaking: the first light from two neutron stars merging

Seven new papers describe the first-ever detection of light from a gravitational wave source. The event, caused by two neutron stars colliding and merging together, was dubbed GW170817 because it sent ripples through space-time that reached Earth on 2017 August 17. Around the world, hundreds of excited astronomers mobilized quickly and were able to observe the event using numerous telescopes, providing a wealth of new data.

Previous detections of gravitational waves have all involved the merger of two black holes, a feat that won the 2017 Nobel Prize in Physics earlier this month....

Im Focus: Smart sensors for efficient processes

Material defects in end products can quickly result in failures in many areas of industry, and have a massive impact on the safe use of their products. This is why, in the field of quality assurance, intelligent, nondestructive sensor systems play a key role. They allow testing components and parts in a rapid and cost-efficient manner without destroying the actual product or changing its surface. Experts from the Fraunhofer IZFP in Saarbrücken will be presenting two exhibits at the Blechexpo in Stuttgart from 7–10 November 2017 that allow fast, reliable, and automated characterization of materials and detection of defects (Hall 5, Booth 5306).

When quality testing uses time-consuming destructive test methods, it can result in enormous costs due to damaging or destroying the products. And given that...

Im Focus: Cold molecules on collision course

Using a new cooling technique MPQ scientists succeed at observing collisions in a dense beam of cold and slow dipolar molecules.

How do chemical reactions proceed at extremely low temperatures? The answer requires the investigation of molecular samples that are cold, dense, and slow at...

Im Focus: Shrinking the proton again!

Scientists from the Max Planck Institute of Quantum Optics, using high precision laser spectroscopy of atomic hydrogen, confirm the surprisingly small value of the proton radius determined from muonic hydrogen.

It was one of the breakthroughs of the year 2010: Laser spectroscopy of muonic hydrogen resulted in a value for the proton charge radius that was significantly...

All Focus news of the innovation-report >>>

Anzeige

Anzeige

Event News

ASEAN Member States discuss the future role of renewable energy

17.10.2017 | Event News

World Health Summit 2017: International experts set the course for the future of Global Health

10.10.2017 | Event News

Climate Engineering Conference 2017 Opens in Berlin

10.10.2017 | Event News

 
Latest News

Terahertz spectroscopy goes nano

20.10.2017 | Information Technology

Strange but true: Turning a material upside down can sometimes make it softer

20.10.2017 | Materials Sciences

NRL clarifies valley polarization for electronic and optoelectronic technologies

20.10.2017 | Interdisciplinary Research

VideoLinks
B2B-VideoLinks
More VideoLinks >>>