The breakthrough, published in the journal Nature Materials, could offer an easier way of detecting pathogenic bacteria outside of a clinical setting and could be particularly important for the developing world, where access to more sophisticated laboratory techniques is often limited.
The research was led by Professor Cameron Alexander, Head of the Division of Drug Delivery and Tissue Engineering and EPSRC Leadership Fellow in the University's School of Pharmacy, building on work by PhD student Peter Magennis.
The image shows a) on the left hand side, aggregates of E coli labelled blue by in situ activated polymers, and b) on the right hand side E. coli clusters in suspension with bacterial-instructed polymers Schematics of the binding process are shown in the enlargement boxes of the image.
Credit: Professor Cameron Alexander The University of Nottingham UK
Professor Alexander said: "Essentially, we have hijacked some of the metabolic machinery which bacteria use to control their environment, and used it instead to grow polymers which bind strongly to the specific bacteria that produce them.
"The neat thing about this is that the functionality of the polymers grown on the surface of the bacteria is programmed by the cells so that they can recognise their own 'kind'. We used fluorescent labels to light up the polymers and were able to capture this labelling using a mobile phone camera, so in principle it could be possible to use these materials as point-of-care diagnostics for pathogenic bacteria."
The study has shown that the bacteria helped to synthesise polymers on their own surfaces which not only were different from those made by conventional methods, but which retained a form of 'structural memory' of that surface. This means in future it should be possible to make specific detection agents or additives for topical anti-infectives that target a number of harmful bacteria all by a common route.
"The initial focus of the research was to explore ways to use synthetic polymers to selectively target and bind the bacteria that cause dental cavities and periodontal diseases in order to facilitate their removal from the oral cavity," said Dr David Churchley, Principal Scientist, Oral Health Category Research and Development, GSK Consumer Healthcare. "As we continued our work, we saw that our research had broader implications and potential for a wider range of uses."
Rapidly identifying harmful bacteria at the heart of a serious medical or dental condition can be a difficult and costly task. The group's findings may even lead to new ways of treating bacterial infections. "These types of polymers may be designed to contain antibacterial functionalities so that they specifically bind to and kill bacterial pathogens," said Dr Klaus Winzer, a microbiologist at The University of Nottingham involved in the study. The selective binding of specific bacterial species and/or strains in current practice requires expensive 'cold-chain' reagents such as antibodies which often preclude using these processes outside of a hospital setting or in developing nations.
The new approach, termed 'bacterial-instructed synthesis', has the potential for use in the developing world, in the field or in less specialised laboratory settings.
Dr David Bradshaw, Principal Scientist, Oral Health Category Research and Development, GSK Consumer Healthcare, said: "The ingredients used to form the polymers are all easy to obtain, inexpensive and widely available. With the simplicity and accessibility of the chemistry, a number of diagnostic and other applications may be possible."
The study was funded by a Biotechnology and Biological Sciences Research Council (BBSRC) GSK Consumer Healthcare CASE studentship, and Professor Alexander's Engineering and Physical Research Council (EPSRC) Leadership Fellowship.
The paper will be accessible on the Nature Materials website (after the embargo has lifted) at http://dx.doi.org/10.1038/nmat3949
Emma Thorne | Eurek Alert!
Fish Oil-Diet Benefits May be Mediated by Gut Microbes
28.08.2015 | University of Gothenburg
Bio-fabrication of Artificial Blood Vessels with Laser Light
28.08.2015 | Fraunhofer-Institut für Lasertechnik ILT
A University of Oklahoma astrophysicist and his Chinese collaborator have found two supermassive black holes in Markarian 231, the nearest quasar to Earth, using observations from NASA's Hubble Space Telescope.
The discovery of two supermassive black holes--one larger one and a second, smaller one--are evidence of a binary black hole and suggests that supermassive...
A team of European researchers have developed a model to simulate the impact of tsunamis generated by earthquakes and applied it to the Eastern Mediterranean. The results show how tsunami waves could hit and inundate coastal areas in southern Italy and Greece. The study is published today (27 August) in Ocean Science, an open access journal of the European Geosciences Union (EGU).
Though not as frequent as in the Pacific and Indian oceans, tsunamis also occur in the Mediterranean, mainly due to earthquakes generated when the African...
In mountainous regions earthquakes often cause strong landslides, which can be exacerbated by heavy rain. However, after an initial increase, the frequency of these mass wasting events, often enormous and dangerous, declines, in fact independently of meteorological events and aftershocks.
These new findings are presented by a German-Franco-Japanese team of geoscientists in the current issue of the journal Geology, under the lead of the GFZ...
Bacteria do not cease to amaze us with their survival strategies. A research team from the University of Basel's Biozentrum has now discovered how bacteria enter a sleep mode using a so-called FIC toxin. In the current issue of “Cell Reports”, the scientists describe the mechanism of action and also explain why their discovery provides new insights into the evolution of pathogens.
For many poisons there are antidotes which neutralize their toxic effect. Toxin-antitoxin systems in bacteria work in a similar manner: As long as a cell...
It comes when called, bringing care utensils with it and recording how they are used: Fraunhofer IPA is developing an intelligent care cart that provides care staff with physical and informational support in their day-to-day work. The scientists at Fraunhofer IPA have now completed a first prototype. In doing so, they are continuing in their efforts to improve working conditions in the care sector and are developing solutions designed to address the challenges of demographic change.
Technical assistance systems can improve the difficult working conditions in residential nursing homes and hospitals by helping the staff in their work and...
20.08.2015 | Event News
20.08.2015 | Event News
19.08.2015 | Event News
31.08.2015 | Earth Sciences
28.08.2015 | Physics and Astronomy
28.08.2015 | Health and Medicine