Genome analysis of marine microbe reveals a metabolic minimalist
Flightless birds, blind cave shrimp, and other oddities suggest a "use it or lose it" tendency in evolution. In the microbial world, an unusual marine microorganism appears to have ditched several major metabolic pathways, leaving it with a remarkably reduced set of genes.
This metabolic minimalist is a specialist uniquely suited to performing one very important function: taking nitrogen gas from the atmosphere and "fixing" it into a form that makes this essential nutrient available to other organisms. Nitrogen fixation fertilizes the oceans, controlling overall biological productivity and thereby affecting how much carbon dioxide the oceans absorb from the atmosphere.
Jonathan Zehr, the marine microbiologist who discovered the microbe, said it has stubbornly resisted efforts to grow it in the laboratory. But that hasn't stopped his team from determining the complete DNA sequence of its genome. Genome analysis enabled the researchers to reconstruct the organism's unusual metabolic lifestyle. They published their findings in Nature in a paper available online February 21.
Zehr, a professor of ocean sciences at the University of California, Santa Cruz, characterized the microbe as an atypical member of the cyanobacteria, a group of photosynthetic bacteria formerly known as blue-green algae. Still lacking a formal taxonomic classification, it is known only as UCYN-A. First detected in the open ocean near Hawaii in 1998, it is now known to be periodically abundant in tropical and subtropical waters throughout the world.
"Biogeochemists have never been able to balance the nitrogen budget of the oceans--there seems to be more nitrogen produced than we can account for from known organisms. So this organism may be an important part of the overall nitrogen budget," Zehr said.
In a 2008 paper in Science, Zehr's team reported that UCYN-A is completely lacking the genes for a key component of the photosynthetic apparatus . The missing parts, known as photosystem II, carry out the stage in photosynthesis that generates oxygen by splitting water molecules. This is significant because oxygen inhibits nitrogen fixation. Most nitrogen-fixing cyanobacteria carry out photosynthesis during the day and nitrogen fixation at night, but UCYN-A can fix nitrogen all day long.
The new paper extends the list of UCYN-A's missing metabolic pathways to include, among other things, a process central to aerobic metabolism known as the TCA cycle or Krebs cycle. It also lacks the Calvin cycle, which uses the carbon from carbon dioxide to build sugars, and it is unable to synthesize about half of the 20 essential amino acids.
"This thing is really stripped down," said James Tripp, a bioinformatics specialist at UCSC and lead author of the Nature paper. "My analysis indicates it has to have an outside source to obtain sugars, amino acids, and two out of the four bases needed to make DNA."
Tripp performed the genome analysis reported in the paper. He worked closely with scientists at 454 Life Sciences, a Roche company based in Branford, Conn., that specializes in high-throughput DNA sequencing technology. The researchers applied new genome sequencing and assembly techniques to produce the complete genome sequence from natural samples of DNA. Because UCYN-A cannot be cultured, researchers used a cell-sorting technique called flow cytometry to obtain concentrated samples of the microbe from ocean water, and then extracted DNA from the cells for sequencing.
Although UCYN-A must depend on other organisms for key nutrients, the researchers have found no evidence that it lives in a close symbiotic association with another microorganism. Zehr said the failure to find another organism closely associated with it suggests two possibilities. "It might live in a cryptic association that's very hard to sample because it's fragile and just falls apart, or it may respond to blooms of other phytoplankton and live in the soup of nutrients excreted by other organisms," he said.
One of the striking things about UCYN-A's metabolism is that it lacks essential pathways other organisms use to generate energy for their cells. "Nitrogen fixation takes a lot of energy, but this thing has figured out how to fix nitrogen without the normal pathways used to fuel it in other organisms," Zehr said. "It presents a real evolutionary and ecological paradox."
In addition to Zehr and Tripp, the coauthors of the Nature paper include graduate student Shellie Bench, specialist Kendra Turk, and postdoctoral scholar Rachel Foster in Zehr's lab at UCSC, and Brian Desany, Faheem Niazi, and Jason Affourtit of 454 Life Sciences. This research was supported by the Gordon and Betty Moore Foundation, the National Science Foundation, and the NSF Center for Microbial Oceanography Research and Education.
Tim Stephens | EurekAlert!
The most recent press releases about innovation >>>
Die letzten 5 Focus-News des innovations-reports im Überblick:
University of Maryland researchers contribute to historic detection of gravitational waves and light created by event
On August 17, 2017, at 12:41:04 UTC, scientists made the first direct observation of a merger between two neutron stars--the dense, collapsed cores that remain...
Seven new papers describe the first-ever detection of light from a gravitational wave source. The event, caused by two neutron stars colliding and merging together, was dubbed GW170817 because it sent ripples through space-time that reached Earth on 2017 August 17. Around the world, hundreds of excited astronomers mobilized quickly and were able to observe the event using numerous telescopes, providing a wealth of new data.
Previous detections of gravitational waves have all involved the merger of two black holes, a feat that won the 2017 Nobel Prize in Physics earlier this month....
Material defects in end products can quickly result in failures in many areas of industry, and have a massive impact on the safe use of their products. This is why, in the field of quality assurance, intelligent, nondestructive sensor systems play a key role. They allow testing components and parts in a rapid and cost-efficient manner without destroying the actual product or changing its surface. Experts from the Fraunhofer IZFP in Saarbrücken will be presenting two exhibits at the Blechexpo in Stuttgart from 7–10 November 2017 that allow fast, reliable, and automated characterization of materials and detection of defects (Hall 5, Booth 5306).
When quality testing uses time-consuming destructive test methods, it can result in enormous costs due to damaging or destroying the products. And given that...
Using a new cooling technique MPQ scientists succeed at observing collisions in a dense beam of cold and slow dipolar molecules.
How do chemical reactions proceed at extremely low temperatures? The answer requires the investigation of molecular samples that are cold, dense, and slow at...
Scientists from the Max Planck Institute of Quantum Optics, using high precision laser spectroscopy of atomic hydrogen, confirm the surprisingly small value of the proton radius determined from muonic hydrogen.
It was one of the breakthroughs of the year 2010: Laser spectroscopy of muonic hydrogen resulted in a value for the proton charge radius that was significantly...