Their study is published in issue 19(3) of Cell Transplantation and it is now freely available on-line at http://www.ingentaconnect.com/content/cog/ct/ .
"Inconsistent islet isolation is one of the important issues in clinical islet transplantation," said Dr. Shinichi Matsumoto, the research team's lead author. "Failure of donor islet isolation often results from the loss of the donor pancreas. Our simple modification of the retrieval process appears valuable for assuring greater success in islet transplantation."
Ductal injection is a procedure that modifies the islet isolation process using a cooling solution on the pancreatic islet cells derived from brain-dead donors. The cooling solution, applied at the donor's pancreatic ductal site, aids the viability of the islet cells.
The team successfully isolated islet cells in the DI group seven times while only three out of eight islet cell groups were isolated in the nonductal injection group. When islets from the DI group were transplanted into three type 1 diabetic patients, all three became insulin independent.
"DI significantly improved the quantity and quality of isolated islets and resulted in a high success rate of clinical islet transplantation," said Dr. Matsumoto.
According to the research team, a fifty percent success rate for clinical islet isolation has been standard; they were able to achieve a better than 80 percent success rate using DI.
The team reported that there were no significant demographic or clinical differences in the two patient groups receiving islet transplants, nor were there significant differences in the donated pancreata. All donor pancreata were preserved for less than six hours. Each patient received two islet preparations.
"In the DI group, the fasting blood glucose of all three patients improved after a single islet transplantation, and improved further after the second transplantation," commented Dr. Matsumoto. "None of these patients experienced subsequent hypoglycemia, and all three became insulin independent."
The team had recently shown that the DI process was successful in animal models because DI prevented tissue and cell death, suggesting that DI improved the quality and quantity of the isolated islet cells destined for transplantation.
"The number of islets isolated from donor pancreata continues to be quite variable and many times are not sufficient for clinical transplantation" said Dr. Rodolfo Alejandro, section editor for Cell Transplantation and Professor of Medicine at the University of Miami Miller School of Medicine. "This paper describes a novel approach to improve islet isolation yields. These are promising results that need to be confirmed in a randomized concurrent protocol".
Contact: Dr. Shinichi Matsumoto, Baylor All Saints Medical Center, Baylor Research Institute 1400 8th Avenue, Fort Worth, Texas 76104, USA. Tel: 817-922-2570 Fax 817-922-4645 Email: email@example.com
The editorial offices for Cell Transplantation are at the Center of Excellence for Aging and Brain Repair, College of Medicine, the University of South Florida and the Diabetes Research Institute, University of Miami Miller School of Medicine. Contact, David Eve, PhD. at firstname.lastname@example.org or Camillo Ricordi, MD at email@example.com
News release by Randolph Fillmore, www.sciencescribe.net
David Eve | EurekAlert!
Link Discovered between Immune System, Brain Structure and Memory
26.04.2017 | Universität Basel
Researchers develop eco-friendly, 4-in-1 catalyst
25.04.2017 | Brown University
More and more automobile companies are focusing on body parts made of carbon fiber reinforced plastics (CFRP). However, manufacturing and repair costs must be further reduced in order to make CFRP more economical in use. Together with the Volkswagen AG and five other partners in the project HolQueSt 3D, the Laser Zentrum Hannover e.V. (LZH) has developed laser processes for the automatic trimming, drilling and repair of three-dimensional components.
Automated manufacturing processes are the basis for ultimately establishing the series production of CFRP components. In the project HolQueSt 3D, the LZH has...
Reflecting the structure of composites found in nature and the ancient world, researchers at the University of Illinois at Urbana-Champaign have synthesized thin carbon nanotube (CNT) textiles that exhibit both high electrical conductivity and a level of toughness that is about fifty times higher than copper films, currently used in electronics.
"The structural robustness of thin metal films has significant importance for the reliable operation of smart skin and flexible electronics including...
The nearby, giant radio galaxy M87 hosts a supermassive black hole (BH) and is well-known for its bright jet dominating the spectrum over ten orders of magnitude in frequency. Due to its proximity, jet prominence, and the large black hole mass, M87 is the best laboratory for investigating the formation, acceleration, and collimation of relativistic jets. A research team led by Silke Britzen from the Max Planck Institute for Radio Astronomy in Bonn, Germany, has found strong indication for turbulent processes connecting the accretion disk and the jet of that galaxy providing insights into the longstanding problem of the origin of astrophysical jets.
Supermassive black holes form some of the most enigmatic phenomena in astrophysics. Their enormous energy output is supposed to be generated by the...
The probability to find a certain number of photons inside a laser pulse usually corresponds to a classical distribution of independent events, the so-called...
Microprocessors based on atomically thin materials hold the promise of the evolution of traditional processors as well as new applications in the field of flexible electronics. Now, a TU Wien research team led by Thomas Müller has made a breakthrough in this field as part of an ongoing research project.
Two-dimensional materials, or 2D materials for short, are extremely versatile, although – or often more precisely because – they are made up of just one or a...
20.04.2017 | Event News
18.04.2017 | Event News
03.04.2017 | Event News
26.04.2017 | Life Sciences
26.04.2017 | Physics and Astronomy
26.04.2017 | Earth Sciences