Forum for Science, Industry and Business

Sponsored by:     3M 
Search our Site:

 

Bitter-tasting nectar and floral odors optimize outcrossing for plants

29.08.2008
Experiments with genetically modified plants reveal new aspects on the biochemistry of flowers

Animals "personally" bring their gametes together - seeking out sexual partners, mating, fertilizing, and reproducing. Plants, however, are sessile organisms and require the help of a third party, the pollinator, which can be a bird, mouse or insect that transport pollen to receptive stigmas frequently over large distances.


Flower visitor Selasphorus rufus
Danny Kessler, MPI Chemical Ecology


Flower components nicotine (N), a repellent present in the nectar, and benzyl acetone (BA), a volatile attractant released by petals (right). Both components optimize the plant\'s production of outcrossed seeds
Danny Kessler, MPI Chemical Ecology

The colors and shapes of flowers as well as their volatile signals and nectar attract and reward the pollinators for their efforts. But not all flower visitors are pollinators, as many come only to steal nectar without transporting pollen or eat flower parts. Scientists at the Max Planck Institute for Chemical Ecology, Jena, have discovered that the chemistry of floral scents and nectar enforces good pollinator behavior and allows plants to optimize their ability to exchange gametes with each other.

In a paper published in the latest issue of SCIENCE (August 29, 2008), the scientists report the results from field experiments with genetically modified wild tobacco plants that show that particular components of the floral fragrance attracted pollinators, while bitter-tasting and poisonous components of floral nectar enforced modest drinking behavior. Apart from sugars, the floral nectar of tobacco also contains nicotine, which is bitter and used to deter nectar thieves or herbivores. But given the right timing and dose, nicotine in the nectar and the attractant, benzyl acetone, released at night as part of the floral scent, ensure that the visits of pollinators such as hummingbirds and hawk moths optimize the tobacco plant's ability to exchange gametes and produce outcrossed seeds.

Prior to these field tests, the researchers showed that the amount of nicotine in the floral nectar of wild tobacco influenced pollination by the tobacco hornworm Manduca sexta and two hummingbird species [1]. To understand the floral biochemistry and plants' ecological interactions with their mobile visitors, the researchers generated four different lines of genetically modified wild tobacco (Nicotiana attenuata). Apart from control plants that had received only a blind copy of the transgenic DNA fragment, transgenic lines were created (by means of RNA interference) that were unable to produce either nicotine or benzyl acetone; the latter has a sweet odor we know from cocoa beans and is similar to the smell of jasmine and strawberry. A fourth line of transgenic plants could produce neither nicotine nor benzyl acetone.

After a series of control experiments in the field showed that the genetic modifications per se did not influence growth, flower formation, nectar production, or the frequency of outcrossing, the scientists ran a series of three tests: First, measurements of floral nectar showed that its volume was only half the size in transgenic plants which were impaired in nicotine production, compared to control plants and the lines that could not produce benzyl acetone as an attractant. Conclusion: Floral visitors are principally attracted by the scent, and they drink more nectar if it doesn't taste bitter. Using video cameras, the researchers confirmed this result: Both attractant-deficient lines were rarely visited by hummingbirds (e.g. Archilochus alexandri) and white-lined sphinx moths (Hyles lineata). When visitors took nectar from flowers which contained a natural amount of deterring nicotine, they stayed for a short time only, while they enjoyed the nicotine-free nectar of corresponding transgenic lines. Consequently, these flowers were visited for longer periods, especially by hummingbirds.

However, such observations do not prove that different visiting behaviors affect outcrossing and reproduction among plants. Therefore, two further analyses were performed, one focused on female fitness (production rate of seeds in the ovary), the other on male fitness (successful pollination of neighboring plants).

To determine female fitness, the flowers of the four transgenic plant lines were emasculated by removing the anthers. This enabled the researchers to measure only animal-mediated fertilization success rates, because self-pollination was prevented - a method utilized by plant breeders. It could be shown that only the control plants were normally cross-pollinated by pollen of the surrounding wild-grown tobacco plants, whereas the transgenic nicotine- and benzyl acetone-deficient lines could only produce less than half of the seeds.

The scientists measured the male fitness of the four transgenic lines by emasculating flowers of plants and subsequently determining the origin of pollen which had fertilized their seeds with DNA probes. This paternity test allowed scientists to identify which of the transgenic plant lines were most successful at passing their pollen along to neighboring plants. Here it could also be shown that the control plants producing natural amounts of nicotine and benzyl acetone were the most potent ones; the big losers (almost five times less of cross-fertilized seeds) were the plants that produced neither nicotine nor benzyl acetone.

Interestingly, during the growing season, the male fertilization success switched from the attractant (benzyl acetone)-deficient to the nicotine-deficient plants. In other words: The influence of nicotine in the nectar on successful pollinator-mediated fertilization of tobacco plants decreased continuously, whereas the attractant became more and more important. These measurements were confirmed by video recordings which showed that early in the year, when hummingbirds visit tobacco, nicotine in the nectar causes them to drink less of the bitter nectar, and in turn visit other flowers, thus increasing pollen transfer. Later in the year, moths visit frequently, attracted by the odor of benzyl acetone. The bitter taste of the nectar doesn't seem to bother them.

The leader of the studies, Ian Baldwin, notes that just as the manufacturers of soft drinks protect their formulas and strive for constancy in order not to lose market share, altering their recipes only in response to the dictates of global sales, so plants evolve and incorporate ingredients into their nectar recipes in response to the dictates of their Darwinian fitness. "Nectar, which was thought to be nature's soft drink, may not be so soft after all," Baldwin says. Unlike animals, plants are sessile, and through chemistry, flowers can optimize visitors' behavior.

The scientists also observed that nicotine in the nectar deters flower-eating insects which have a straightforward negative impact on reproduction. Odorant attractants lure not only pollinators but also herbivores. Tobacco plants seem to solve this dilemma by using nicotine as a deterrent.

The experiments with flowering transgenic tobacco plants were conducted at MPI's field station operated at Brigham Young University's Lytle Ranch Preserve in Utah, USA, and were monitored by the responsible authorities (USDA-APHIS).

Keyword: Chemical Ecology
Chemical ecology is a young discipline among the life sciences. Interactions, harmful as well as beneficial, are mediated by chemical signals between organisms. At the Max Planck Institute for Chemical Ecology, researchers examine the structure and function of molecules that regulate the interplay among plants, insects and microbes, and seek insight into the growth, development, behavior, and co-evolution of plant and animal species. The results of this basic biological research are used for the analysis of natural products, modern environmental analyses, and the development of state-of-the-art agricultural techniques. The institute is equipped with greenhouses, climate chambers, insect-rearing facilities, odor detection systems, wind tunnels, neurophysiological instruments, and field stations.
Original Publication:
Danny Kessler, Klaus Gase, Ian T. Baldwin: Field experiments with transformed plants reveal the sense of floral scents.

SCIENCE, August 29, 2008

Further Reading:
[1] Kessler D; Baldwin IT (2007): Making sense of nectar scents: the effects of nectar secondary metabolites on floral visitors of Nicotiana attenuata. The Plant Journal 49, 840-854
Further Information:
Prof. Ian T. Baldwin, Max Planck Institute for Chemical Ecology, Jena,
Tel. +49 (0)175 1804226; +49 (0)3641 57-1100, 1101, 1000; baldwin@ice.mpg.de
Picture Material:
Angela Overmeyer M.A., Max Planck Institute for Chemical Ecology, Jena,
Tel.: +49 (0)3641 57-2110; overmeyer@ice.mpg.de

Dr. Jan-Wolfhard Kellmann | Max-Planck-Gesellschaft
Further information:
http://www.ice.mpg.de

More articles from Life Sciences:

nachricht Single-stranded DNA and RNA origami go live
15.12.2017 | Wyss Institute for Biologically Inspired Engineering at Harvard

nachricht New antbird species discovered in Peru by LSU ornithologists
15.12.2017 | Louisiana State University

All articles from Life Sciences >>>

The most recent press releases about innovation >>>

Die letzten 5 Focus-News des innovations-reports im Überblick:

Im Focus: First-of-its-kind chemical oscillator offers new level of molecular control

DNA molecules that follow specific instructions could offer more precise molecular control of synthetic chemical systems, a discovery that opens the door for engineers to create molecular machines with new and complex behaviors.

Researchers have created chemical amplifiers and a chemical oscillator using a systematic method that has the potential to embed sophisticated circuit...

Im Focus: Long-lived storage of a photonic qubit for worldwide teleportation

MPQ scientists achieve long storage times for photonic quantum bits which break the lower bound for direct teleportation in a global quantum network.

Concerning the development of quantum memories for the realization of global quantum networks, scientists of the Quantum Dynamics Division led by Professor...

Im Focus: Electromagnetic water cloak eliminates drag and wake

Detailed calculations show water cloaks are feasible with today's technology

Researchers have developed a water cloaking concept based on electromagnetic forces that could eliminate an object's wake, greatly reducing its drag while...

Im Focus: Scientists channel graphene to understand filtration and ion transport into cells

Tiny pores at a cell's entryway act as miniature bouncers, letting in some electrically charged atoms--ions--but blocking others. Operating as exquisitely sensitive filters, these "ion channels" play a critical role in biological functions such as muscle contraction and the firing of brain cells.

To rapidly transport the right ions through the cell membrane, the tiny channels rely on a complex interplay between the ions and surrounding molecules,...

Im Focus: Towards data storage at the single molecule level

The miniaturization of the current technology of storage media is hindered by fundamental limits of quantum mechanics. A new approach consists in using so-called spin-crossover molecules as the smallest possible storage unit. Similar to normal hard drives, these special molecules can save information via their magnetic state. A research team from Kiel University has now managed to successfully place a new class of spin-crossover molecules onto a surface and to improve the molecule’s storage capacity. The storage density of conventional hard drives could therefore theoretically be increased by more than one hundred fold. The study has been published in the scientific journal Nano Letters.

Over the past few years, the building blocks of storage media have gotten ever smaller. But further miniaturization of the current technology is hindered by...

All Focus news of the innovation-report >>>

Anzeige

Anzeige

Event News

See, understand and experience the work of the future

11.12.2017 | Event News

Innovative strategies to tackle parasitic worms

08.12.2017 | Event News

AKL’18: The opportunities and challenges of digitalization in the laser industry

07.12.2017 | Event News

 
Latest News

Diamond Lenses and Space Lasers at Photonics West

15.12.2017 | Trade Fair News

A better way to weigh millions of solitary stars

15.12.2017 | Physics and Astronomy

New epidemic management system combats monkeypox outbreak in Nigeria

15.12.2017 | Information Technology

VideoLinks
B2B-VideoLinks
More VideoLinks >>>