University of Arizona scientists have used a new quick-growing technique to produce a water-soluble form of a plant compound that combats cancer and encourages the survival of healthy cells.
Research trials are under way on this sulfate form of withaferin A, which could develop into a new anti-cancer drug.
Scientifically studied since the 1960s, withaferin A reduces tumor mass by preventing the growth of blood vessels that make a tumor malignant. The compound is derived from the roots of a winter cherry plant, the extracts of which have been used for more than 3,000 years in India as a general tonic to build stamina, improve mental concentration, relieve stress and enhance health.
"Finding a water-soluble analog of withaferin A is significant, especially if it turns out to be a clinically useful drug," said Leslie Gunatilaka, director of the UA's Southwest Center for Natural Products Research and Commercialization, or Natural Products Center.
Withania is widely cultivated for commercial use in its native India, and also in the Middle East and in North America. It often is sold under the name ashwagandha as a dietary supplement in the U.S. and Europe.
Although traditionally grown outdoors in soil, the UA team decided to use an entirely nontraditional method - aeroponics - to produce bulk amounts of withaferin A needed for biological evaluation.
In aeroponics, plants are set over enclosed chambers where their suspended roots are misted with water and nutrients, instead of growing in soil.
The withania plants grew about five times larger using this method than if they had been grown in soil.
"Using the aeroponic system for cultivation, we were able to produce more than 20 grams of withaferin A in a single greenhouse. It normally costs around $195 for just 10 milligrams," Gunatilaka said. "Also, it usually takes two to three years to mature to sizeable roots to be commercially viable, but here it takes just six to nine months."
Not only did the aeroponic method yield bigger plants faster, with more withaferin A than usual, it also unexpectedly stimulated the plants to produce large amounts of the new natural product - a water-soluble sulfate form of withaferin A.
Upon testing, this new form demonstrated the same bioactivity as withaferin A. It was able to inhibit the proliferation and survival of tumor cells, disrupt tumor formation and induce the healthy cells' heat-shock response to reduce stress and increase survival, according to the researchers.
The difference is that the sulfate form of withaferin A is slower acting and water-soluble; it can be converted to withaferin A in cell culture media. The researchers, expecting that this withaferin A form will convert to its active form when metabolized in the body, are pursuing further testing in animal models. The patent will be held by the UA and the Massachusetts Institute of Technology.
Natural Products Center scientists collaborated with researchers from the Whitehead Institute at MIT on this project. In addition to Gunatilaka, the researchers included Ya-ming Xu, Marilyn T. Marron, Emily Seddon and Stephen P. McLaughlin of the Natural Products Center; Dennis Ray of the UA School of Plant Sciences; and Luke Whitesell of the Whitehead Institute.
The UA College of Agriculture and Life Sciences provided funding for the project, along with the USDA.
Withaferin A is just one of hundreds of such compounds that scientists with the Natural Products Center have isolated, characterized and evaluated since the center's inception in 1996. Center researchers look for compounds in desert plants and their associated microorganisms that can improve human health and be developed as potential industrial products in Arizona.
Natural Products Center scientists have discovered several other compounds in desert organisms that can significantly inhibit the growth of tumors. Many have been patented and have progressed to extended evaluation for their pharmaceutical value.
As part of the Office of Arid Lands Studies, the Natural Products Center recently joined the School of Natural Resources and the Environment in the UA's College of Agriculture and Life Sciences.
Leslie Gunatilaka, Natural Products Center (520-621-9932; email@example.com)
Susan McGinley, College of Agriculture and Life Sciences (520-621-7182; firstname.lastname@example.org)
Jennifer Fitzenberger | University of Arizona
Gene therapy shows promise for treating Niemann-Pick disease type C1
27.10.2016 | NIH/National Human Genome Research Institute
'Neighbor maps' reveal the genome's 3-D shape
27.10.2016 | International School of Advanced Studies (SISSA)
Ultrafast lasers have introduced new possibilities in engraving ultrafine structures, and scientists are now also investigating how to use them to etch microstructures into thin glass. There are possible applications in analytics (lab on a chip) and especially in electronics and the consumer sector, where great interest has been shown.
This new method was born of a surprising phenomenon: irradiating glass in a particular way with an ultrafast laser has the effect of making the glass up to a...
Terahertz excitation of selected crystal vibrations leads to an effective magnetic field that drives coherent spin motion
Controlling functional properties by light is one of the grand goals in modern condensed matter physics and materials science. A new study now demonstrates how...
Researchers from the Institute for Quantum Computing (IQC) at the University of Waterloo led the development of a new extensible wiring technique capable of controlling superconducting quantum bits, representing a significant step towards to the realization of a scalable quantum computer.
"The quantum socket is a wiring method that uses three-dimensional wires based on spring-loaded pins to address individual qubits," said Jeremy Béjanin, a PhD...
In a paper in Scientific Reports, a research team at Worcester Polytechnic Institute describes a novel light-activated phenomenon that could become the basis for applications as diverse as microscopic robotic grippers and more efficient solar cells.
A research team at Worcester Polytechnic Institute (WPI) has developed a revolutionary, light-activated semiconductor nanocomposite material that can be used...
By forcefully embedding two silicon atoms in a diamond matrix, Sandia researchers have demonstrated for the first time on a single chip all the components needed to create a quantum bridge to link quantum computers together.
"People have already built small quantum computers," says Sandia researcher Ryan Camacho. "Maybe the first useful one won't be a single giant quantum computer...
14.10.2016 | Event News
14.10.2016 | Event News
12.10.2016 | Event News
27.10.2016 | Materials Sciences
27.10.2016 | Physics and Astronomy
27.10.2016 | Life Sciences