CNMPB scientists develop new tool to analyze cellular structures via high- resolution imaging. Published May 26th, 2014 in the Journal of Cell Biology.
In the past two decades, super-resolution microscopy has been one of the fastest evolving fields through many technical improvements. However, the development of new labeling tools, probes and their biological application, is mostly lagging behind the technical capabilities.
From left to right: Prof. Dr. Silvio O. Rizzoli, Natalia Revelo, Dr. Dirk Kamin, Sven Truckenbrodt.
Most recently, Prof. Silvio O. Rizzoli from the Cluster of Excellence and DFG- Research Center for Nanoscale Microscopy and Molecular Physiology of the Brain (CNMPB) has developed together with his team a new technique that expands the benefit of super- resolution microscopy to study biological questions.
This method contributes to understand on how cells renew, distribute and transport their molecular and subcellular components. The new technique was published on May 26th in the Journal of Cell Biology.
Revelo NH, Kamin D, Truckenbrodt S, Wong AB, Reuter K, Reisinger E, Moser T, Rizzoli SO (2014) A new probe for super-resolution imaging of membranes elucidates trafficking pathways. J CELL BIOL, May 26; 205(4): 591-606.
All cells rely on the recycling of membranes via various pathways (secretion, uptake, and membrane turnover). Several types of cellular organelles such as the plasma membrane, the endoplasmic reticulum, the Golgi apparatus, endosomes and vesicles are involved in these processes.
However, it was difficult to identify the protein composition of the involved organelles since both, the membranes and the proteins of the same organelle need to be marked simultaneously. Here the main difficulty comes with the membrane probe, as almost all dyes that work excellent in live cell experiments are only poorly fixable and get “lost” during the antibody staining procedure.
The research team with first author Natalia Revelo therefore developed a membrane probe that overcomes this problem. The probe mCLING (membrane-binding fluorophore-Cysteine- Lysine-Palmitoyl Group) is a composition of a short polypeptide coupled to a membrane anchor and a fluorophore.
The study, recently published in the Journal of Cell Biology, shows that mCLING can be used to label the plasma membrane, and also to faithfully track specific organelles, which can be done in conjunction with fixation and immunostaining, in both cell culture and in tissue.
The utility of the mCLING probe could be characterized for various important biological model systems and already enabled the authors to answer long-lasting question in the field of membrane recycling. Moreover, mCLING imaging could also be extended to different processes.
For example, the structure and molecular organization of isolated organelles in vitro, or the arrangement of proteins on the membranes of various types of cells, can be easily tackled with mCLING. These efforts will be aided by the fact that mCLING can be optimized for any available super-resolution technique.
Prof. Dr. Silvio O. Rizzoli is head of the Department of Neuro- and Sensory Physiology at the University Medical Center Göttingen and member of the Göttingen Cluster of Excellence and DFG Research Center for Nanoscale Microscopy and Molecular Physiology of the Brain (CNMPB). His research focus includes the identification of molecular signal transduction processes between nerve cells.
Prof. Rizzoli applies super-resolution microscopy techniques to study the transport and function of intracellular vesicles in the synapses of nerve cells. Very recently, Prof. Rizzoli received for the second time with a prestigious funding award of the European Union for his excellent research proposal.
Prof. Dr. Silvio O. Rizzoli
University Medical Center Göttingen Department Neuro- & Sensory Physiology
c/o European Neuroscience Institute (ENI) Grisebachstraße 5, 37077 Göttingen
Phone 0551 / 39-33630, email@example.com
CNMPB – Center for Nanoscale Microscopy and Molecular Physiology of the Brain Cluster of Excellence 171 – DFG Research Center 103
Dr. Heike Conrad
Scientific Coordination, Press & Public Relations
Humboldtallee 23, 37073 Göttingen
Phone 0551 / 39-7065, firstname.lastname@example.org
Dr. Heike Conrad | idw - Informationsdienst Wissenschaft
Flow of cerebrospinal fluid regulates neural stem cell division
22.05.2018 | Helmholtz Zentrum München - Deutsches Forschungszentrum für Gesundheit und Umwelt
Chemists at FAU successfully demonstrate imine hydrogenation with inexpensive main group metal
22.05.2018 | Friedrich-Alexander-Universität Erlangen-Nürnberg
So-called quantum many-body scars allow quantum systems to stay out of equilibrium much longer, explaining experiment | Study published in Nature Physics
Recently, researchers from Harvard and MIT succeeded in trapping a record 53 atoms and individually controlling their quantum state, realizing what is called a...
The historic first detection of gravitational waves from colliding black holes far outside our galaxy opened a new window to understanding the universe. A...
A team led by Austrian experimental physicist Rainer Blatt has succeeded in characterizing the quantum entanglement of two spatially separated atoms by observing their light emission. This fundamental demonstration could lead to the development of highly sensitive optical gradiometers for the precise measurement of the gravitational field or the earth's magnetic field.
The age of quantum technology has long been heralded. Decades of research into the quantum world have led to the development of methods that make it possible...
Cardiovascular tissue engineering aims to treat heart disease with prostheses that grow and regenerate. Now, researchers from the University of Zurich, the Technical University Eindhoven and the Charité Berlin have successfully implanted regenerative heart valves, designed with the aid of computer simulations, into sheep for the first time.
Producing living tissue or organs based on human cells is one of the main research fields in regenerative medicine. Tissue engineering, which involves growing...
A team of scientists of the Max Planck Institute for the Structure and Dynamics of Matter (MPSD) at the Center for Free-Electron Laser Science in Hamburg investigated optically-induced superconductivity in the alkali-doped fulleride K3C60under high external pressures. This study allowed, on one hand, to uniquely assess the nature of the transient state as a superconducting phase. In addition, it unveiled the possibility to induce superconductivity in K3C60 at temperatures far above the -170 degrees Celsius hypothesized previously, and rather all the way to room temperature. The paper by Cantaluppi et al has been published in Nature Physics.
Unlike ordinary metals, superconductors have the unique capability of transporting electrical currents without any loss. Nowadays, their technological...
02.05.2018 | Event News
13.04.2018 | Event News
12.04.2018 | Event News
18.05.2018 | Power and Electrical Engineering
18.05.2018 | Information Technology
18.05.2018 | Information Technology