CNMPB scientists develop new tool to analyze cellular structures via high- resolution imaging. Published May 26th, 2014 in the Journal of Cell Biology.
In the past two decades, super-resolution microscopy has been one of the fastest evolving fields through many technical improvements. However, the development of new labeling tools, probes and their biological application, is mostly lagging behind the technical capabilities.
From left to right: Prof. Dr. Silvio O. Rizzoli, Natalia Revelo, Dr. Dirk Kamin, Sven Truckenbrodt.
Most recently, Prof. Silvio O. Rizzoli from the Cluster of Excellence and DFG- Research Center for Nanoscale Microscopy and Molecular Physiology of the Brain (CNMPB) has developed together with his team a new technique that expands the benefit of super- resolution microscopy to study biological questions.
This method contributes to understand on how cells renew, distribute and transport their molecular and subcellular components. The new technique was published on May 26th in the Journal of Cell Biology.
Revelo NH, Kamin D, Truckenbrodt S, Wong AB, Reuter K, Reisinger E, Moser T, Rizzoli SO (2014) A new probe for super-resolution imaging of membranes elucidates trafficking pathways. J CELL BIOL, May 26; 205(4): 591-606.
All cells rely on the recycling of membranes via various pathways (secretion, uptake, and membrane turnover). Several types of cellular organelles such as the plasma membrane, the endoplasmic reticulum, the Golgi apparatus, endosomes and vesicles are involved in these processes.
However, it was difficult to identify the protein composition of the involved organelles since both, the membranes and the proteins of the same organelle need to be marked simultaneously. Here the main difficulty comes with the membrane probe, as almost all dyes that work excellent in live cell experiments are only poorly fixable and get “lost” during the antibody staining procedure.
The research team with first author Natalia Revelo therefore developed a membrane probe that overcomes this problem. The probe mCLING (membrane-binding fluorophore-Cysteine- Lysine-Palmitoyl Group) is a composition of a short polypeptide coupled to a membrane anchor and a fluorophore.
The study, recently published in the Journal of Cell Biology, shows that mCLING can be used to label the plasma membrane, and also to faithfully track specific organelles, which can be done in conjunction with fixation and immunostaining, in both cell culture and in tissue.
The utility of the mCLING probe could be characterized for various important biological model systems and already enabled the authors to answer long-lasting question in the field of membrane recycling. Moreover, mCLING imaging could also be extended to different processes.
For example, the structure and molecular organization of isolated organelles in vitro, or the arrangement of proteins on the membranes of various types of cells, can be easily tackled with mCLING. These efforts will be aided by the fact that mCLING can be optimized for any available super-resolution technique.
Prof. Dr. Silvio O. Rizzoli is head of the Department of Neuro- and Sensory Physiology at the University Medical Center Göttingen and member of the Göttingen Cluster of Excellence and DFG Research Center for Nanoscale Microscopy and Molecular Physiology of the Brain (CNMPB). His research focus includes the identification of molecular signal transduction processes between nerve cells.
Prof. Rizzoli applies super-resolution microscopy techniques to study the transport and function of intracellular vesicles in the synapses of nerve cells. Very recently, Prof. Rizzoli received for the second time with a prestigious funding award of the European Union for his excellent research proposal.
Prof. Dr. Silvio O. Rizzoli
University Medical Center Göttingen Department Neuro- & Sensory Physiology
c/o European Neuroscience Institute (ENI) Grisebachstraße 5, 37077 Göttingen
Phone 0551 / 39-33630, email@example.com
CNMPB – Center for Nanoscale Microscopy and Molecular Physiology of the Brain Cluster of Excellence 171 – DFG Research Center 103
Dr. Heike Conrad
Scientific Coordination, Press & Public Relations
Humboldtallee 23, 37073 Göttingen
Phone 0551 / 39-7065, firstname.lastname@example.org
Dr. Heike Conrad | idw - Informationsdienst Wissenschaft
Cryo-electron microscopy achieves unprecedented resolution using new computational methods
24.03.2017 | DOE/Lawrence Berkeley National Laboratory
How cheetahs stay fit and healthy
24.03.2017 | Forschungsverbund Berlin e.V.
Astronomers from Bonn and Tautenburg in Thuringia (Germany) used the 100-m radio telescope at Effelsberg to observe several galaxy clusters. At the edges of these large accumulations of dark matter, stellar systems (galaxies), hot gas, and charged particles, they found magnetic fields that are exceptionally ordered over distances of many million light years. This makes them the most extended magnetic fields in the universe known so far.
The results will be published on March 22 in the journal „Astronomy & Astrophysics“.
Galaxy clusters are the largest gravitationally bound structures in the universe. With a typical extent of about 10 million light years, i.e. 100 times the...
Researchers at the Goethe University Frankfurt, together with partners from the University of Tübingen in Germany and Queen Mary University as well as Francis Crick Institute from London (UK) have developed a novel technology to decipher the secret ubiquitin code.
Ubiquitin is a small protein that can be linked to other cellular proteins, thereby controlling and modulating their functions. The attachment occurs in many...
In the eternal search for next generation high-efficiency solar cells and LEDs, scientists at Los Alamos National Laboratory and their partners are creating...
Silicon nanosheets are thin, two-dimensional layers with exceptional optoelectronic properties very similar to those of graphene. Albeit, the nanosheets are less stable. Now researchers at the Technical University of Munich (TUM) have, for the first time ever, produced a composite material combining silicon nanosheets and a polymer that is both UV-resistant and easy to process. This brings the scientists a significant step closer to industrial applications like flexible displays and photosensors.
Silicon nanosheets are thin, two-dimensional layers with exceptional optoelectronic properties very similar to those of graphene. Albeit, the nanosheets are...
Enzymes behave differently in a test tube compared with the molecular scrum of a living cell. Chemists from the University of Basel have now been able to simulate these confined natural conditions in artificial vesicles for the first time. As reported in the academic journal Small, the results are offering better insight into the development of nanoreactors and artificial organelles.
Enzymes behave differently in a test tube compared with the molecular scrum of a living cell. Chemists from the University of Basel have now been able to...
20.03.2017 | Event News
14.03.2017 | Event News
07.03.2017 | Event News
24.03.2017 | Materials Sciences
24.03.2017 | Physics and Astronomy
24.03.2017 | Physics and Astronomy