A research team from the Institute of Medical Microbiology, University of Münster, Germany, has developed a method that accelerates resistance testing. In cooperation with an industrial partner, this innovative method can now be optimized for the diagnostic market.
Respiratory, urinary tract, wound infections, and sepsis: the list of sites in which severe and life-threatening diseases manifest as a result of multidrug resistant microorganisms is long. The best option is an antibiotic treatment targeted specifically to the detected pathogen. However, the identification of microorganisms and their resistances to antibiotics is time consuming.
Symbolic picture: Application of microdroplets for rapid determination of resistance using MALDI-TOF mass spectrometry
photo: FZ / E. Deiters-Keul
A research team from the Institute of Medical Microbiology, University of Münster, Germany, has developed a method that considerably accelerates resistance testing. Supported by the German Federal Ministry of Education and Research (BMBF), and in cooperation with an industrial partner, the research team is currently developing this method. Soon to be on the market, it will be readily available for patient management.
The novel method proposed by project leaders Evgeny A. Idelevich, M.D. and Karsten Becker, M.D. is based on MALDI-TOF mass spectrometry, which was recently introduced for microbial identification. Prof. Becker observed, “We also need new techniques for more expedient detection of acquired antibiotic resistance”.
Currently applied methods require more than 24 hours, as they are dependent on bacterial growth. The innovative method from Münster would allow earlier optimization of patient treatment and the prompt initiation of infection control measures, crucial for the protection of hospital patients from a spread of multidrug resistant bacteria.
Currently, many physicians administer broad-spectrum antibiotics to swiftly provide a reliable medicine. Unfortunately, microorganisms can become resistant due to this very treatment. The shift to a more targeted antibiotic therapy reduces antibiotic selection pressure and thus, the emergence of multiresistant bacteria.
Dr. Idelevich added, “The application of MALDI-TOF mass spectrometry for resistance testing was attractive to our research due to its expediency and high accuracy. It can also be optimally combined with the identification process, rendering it cost-efficient”.
Based on this technology, both researchers have developed a rapid and universal method for resistance determination which is independent from the underlying resistance mechanisms and can be performed simultaneously for multiple antibiotics. Through the license agreement with Bruker Daltonik (Bremen, Germany), a pioneer in mass spectrometry technologies, this innovative method can now be developed further and optimized for the diagnostic market.
Prof. Becker commented, “We hope that our method will be available for routine use in clinical laboratories in 2-3 years”. Here in Münster, we are particularly proud of the MALDI-TOF mass spectrometry project, as the MALDI-TOF technology was greatly influenced in the 1980s by Münster scientists. At that time, the groundwork was established for today’s microbial identification. Currently, thousands of laboratories worldwide already use a MALDI-TOF instrument. This sets optimal preconditions for the wide-spread and cost-efficient implementation of our method.”
The cooperation between academic and industrial institutions in Münster and Bremen will be supported by the BMBF for an additional three years; a grant totaling more than 900,000 Euros was awarded to the academic and industrial partners.
Dr. Thomas Bauer
Referent Research and Teaching at Münster University Medical Faculty
Dr. Thomas Bauer | idw - Informationsdienst Wissenschaft
Study points to new drug target in fight against cancer
19.09.2019 | Rice University
Researchers develop tumour growth roadmap
19.09.2019 | Universität Leipzig
How long the battery of your phone or computer lasts depends on how many lithium ions can be stored in the battery's negative electrode material. If the battery runs out of these ions, it can't generate an electrical current to run a device and ultimately fails.
Materials with a higher lithium ion storage capacity are either too heavy or the wrong shape to replace graphite, the electrode material currently used in...
To process information, photons must interact. However, these tiny packets of light want nothing to do with each other, each passing by without altering the...
Researchers from the Department of Atomically Resolved Dynamics of the Max Planck Institute for the Structure and Dynamics of Matter (MPSD) at the Center for Free-Electron Laser Science in Hamburg, the University of Hamburg and the European Molecular Biology Laboratory (EMBL) outstation in the city have developed a new method to watch biomolecules at work. This method dramatically simplifies starting enzymatic reactions by mixing a cocktail of small amounts of liquids with protein crystals. Determination of the protein structures at different times after mixing can be assembled into a time-lapse sequence that shows the molecular foundations of biology.
The functions of biomolecules are determined by their motions and structural changes. Yet it is a formidable challenge to understand these dynamic motions.
At the International Symposium on Automotive Lighting 2019 (ISAL) in Darmstadt from September 23 to 25, 2019, the Fraunhofer Institute for Organic Electronics, Electron Beam and Plasma Technology FEP, a provider of research and development services in the field of organic electronics, will present OLED light strips of any length with additional functionalities for the first time at booth no. 37.
Almost everyone is familiar with light strips for interior design. LED strips are available by the metre in DIY stores around the corner and are just as often...
Later during this century, around 2060, a paradigm shift in global energy consumption is expected: we will spend more energy for cooling than for heating....
19.09.2019 | Event News
10.09.2019 | Event News
04.09.2019 | Event News
20.09.2019 | Life Sciences
20.09.2019 | Life Sciences
20.09.2019 | Life Sciences