Precise diagnosis is based on multiple end-points involving several tests with antibodies or DNA probes for particular biomolecules in a tumor or a virus.
Antibodies and DNA are usually labeled with different fluorescent dyes, generally requiring multiple modes of excitation and detection that can render the measurement slow and laborious. Advances in nanotechnology have led to the emergence of new fluorescent materials, semiconductor nanoparticles (NPs) called "quantum dots", which can be excited by a single light source (wavelength) but that according to their size and composition emit in discrete and separated spectral bands.
The "multiplexing" of such probes is thereby greatly simplified. Scientists working in the Molecular Biology Dept. have shown that single quantum dots can be detected on and in living cells.
The project of the consortium has two elements. The first is the development of other classes of still smaller NPs, i.e. with sizes below 10 nm (less than a millionth of a cm): fluorescent noble-metal "nanodots" and magnetic NPs. These materials are superior to conventional fluorophores in that they exhibit extreme photo- and chemical stability. The nanodots should have reduced toxicity and greater target accessibility than quantum dots, yet offer a similar detection sensitivity. They will be derivatized and tested for specific recognition of biomolecules such as tumor markers (for breast cancer) and global viral disease (Hepatitis C and Dengue Fever). Other core-shell "onion-like" NPs developed by the partner in Santiago de Compostela have diverse and strong magnetic properties and will be tested for their application in micro-chip and MRI diagnostics.
In a parallel effort, several of the partners will optimize the design and performance of a new type of high-speed, sensitive, optically sectioning microscope known as the Programmable Array Microscope (PAM), for use in both the basic research and medical communities. The PAM is very versatile in that it implements many imaging modalities and has been under development in the Molecular Biology Dept. for the past 10 years. It has single-NP sensitivity, and is ideally suited for measurements of thick samples such as tissue slices and patterned arrays, important objects for diagnostic tests.
The FLUOROMAG consortium has been awarded € 2.5 million by the European Union for a period of 3 years. The research project leaders of the consortium are: Donna Arndt-Jovin (MPIbpc, Germany), Arturo López-Quintela (Univ. of Santiago de Compostela, Spain); Vinod Subramaniam (Univ. of Twente, The Netherlands); Quentin Hanley (Univ. of Nottingham Trent, UK). Two small businesses (SMEs) are included in the consortium; Nanogap Sub-nm-powder SA, Spain (Tatiana López del Rio) will produce the NPs in large scale and Cairn Research Ltd., UK (Martin Thomas) will produce and market the newest technical realizations of the PAM.
For further information, contact
Prof. Dr. Donna Arndt-Jovin, Max Planck Institute for Biophysical Chemistry, Dept. Molecular Biology, Am Fassberg 11, 37077 Göttingen, Germany, Phone: +49 (0) 551 201 -1393, Fax: -1467, E-mail: email@example.com
Dr. Joachim Bormann, Max Planck Institute for Biophysical Chemistry, EU Liaison Office, Am Fassberg 11, 37077 Göttingen, Phone: +49 (0) 551 201 -1076, Fax: -1175, E-mail: firstname.lastname@example.org
Dr. Christoph Nothdurft | idw
Chips, light and coding moves the front line in beating bacteria
16.08.2018 | Okinawa Institute of Science and Technology (OIST) Graduate University
Protein droplets keep neurons at the ready and immune system in balance
16.08.2018 | Howard Hughes Medical Institute
Scientists at the University of California, Los Angeles present new research on a curious cosmic phenomenon known as "whistlers" -- very low frequency packets...
Scientists develop first tool to use machine learning methods to compute flow around interactively designable 3D objects. Tool will be presented at this year’s prestigious SIGGRAPH conference.
When engineers or designers want to test the aerodynamic properties of the newly designed shape of a car, airplane, or other object, they would normally model...
Researchers from TU Graz and their industry partners have unveiled a world first: the prototype of a robot-controlled, high-speed combined charging system (CCS) for electric vehicles that enables series charging of cars in various parking positions.
Global demand for electric vehicles is forecast to rise sharply: by 2025, the number of new vehicle registrations is expected to reach 25 million per year....
Proteins must be folded correctly to fulfill their molecular functions in cells. Molecular assistants called chaperones help proteins exploit their inbuilt folding potential and reach the correct three-dimensional structure. Researchers at the Max Planck Institute of Biochemistry (MPIB) have demonstrated that actin, the most abundant protein in higher developed cells, does not have the inbuilt potential to fold and instead requires special assistance to fold into its active state. The chaperone TRiC uses a previously undescribed mechanism to perform actin folding. The study was recently published in the journal Cell.
Actin is the most abundant protein in highly developed cells and has diverse functions in processes like cell stabilization, cell division and muscle...
Scientists have discovered that the electrical resistance of a copper-oxide compound depends on the magnetic field in a very unusual way -- a finding that could help direct the search for materials that can perfectly conduct electricity at room temperatur
What happens when really powerful magnets--capable of producing magnetic fields nearly two million times stronger than Earth's--are applied to materials that...
08.08.2018 | Event News
27.07.2018 | Event News
25.07.2018 | Event News
16.08.2018 | Earth Sciences
16.08.2018 | Life Sciences
16.08.2018 | Materials Sciences