Researchers at Oxford University’s Department of Biochemistry have developed methods for making RNA duplexes and single-stranded RNAs of desired length and sequence. This exciting technology is most applicable to commercial RNA providers and companies with large in-house requirements for RNA molecules as it will greatly increase cost-effectiveness.
Small interfering ribonucleic acids (siRNAs) are powerful laboratory tools for directed post- transcriptional gene expression knockdown and inhibition of viral propagation. For siRNA to be active, it is important that the overhang in the antisense strand is complementary to the target messenger RNA. Exogenous siRNA is frequently used in RNAi studies using chemically synthesised RNA oligonucleotides to identify reagents with optimal activity.
Chemical synthesis of RNAs is relatively straightforward, but can be prohibitively expensive. Intracellular expression provides a source of continuous production of RNA in the cell, but it offers little control over the quantity of the expressed RNA and the sequence length. In vitro transcription is relatively cheap and offers a good approach to synthesis of large quantities of RNA. Unfortunately, in vitro transcription is limited by specific sequence requirements that greatly reduce the number of potential target sites for siRNA selection. Highly efficient promoters cannot be used, due to the leader sequence being transcribed and incorporated into the siRNA, leading to a further disadvantage. The inclusion of these leader sequences ultimately prevents the siRNA from efficiently functioning in RNA interference.
Jennifer Johnson | alfa
NTU Singapore scientists convert plastics into useful chemicals using su
12.12.2019 | Nanyang Technological University
Eavesdropping on the human microbiome uncovers 'potent' new antibiotics
12.12.2019 | Princeton University
In a joint experimental and theoretical work performed at the Heidelberg Max Planck Institute for Nuclear Physics, an international team of physicists detected for the first time an orbital crossing in the highly charged ion Pr⁹⁺. Optical spectra were recorded employing an electron beam ion trap and analysed with the aid of atomic structure calculations. A proposed nHz-wide transition has been identified and its energy was determined with high precision. Theory predicts a very high sensitivity to new physics and extremely low susceptibility to external perturbations for this “clock line” making it a unique candidate for proposed precision studies.
Laser spectroscopy of neutral atoms and singly charged ions has reached astonishing precision by merit of a chain of technological advances during the past...
The ability to investigate the dynamics of single particle at the nano-scale and femtosecond level remained an unfathomed dream for years. It was not until the dawn of the 21st century that nanotechnology and femtoscience gradually merged together and the first ultrafast microscopy of individual quantum dots (QDs) and molecules was accomplished.
Ultrafast microscopy studies entirely rely on detecting nanoparticles or single molecules with luminescence techniques, which require efficient emitters to...
Graphene, a two-dimensional structure made of carbon, is a material with excellent mechanical, electronic and optical properties. However, it did not seem suitable for magnetic applications. Together with international partners, Empa researchers have now succeeded in synthesizing a unique nanographene predicted in the 1970s, which conclusively demonstrates that carbon in very specific forms has magnetic properties that could permit future spintronic applications. The results have just been published in the renowned journal Nature Nanotechnology.
Depending on the shape and orientation of their edges, graphene nanostructures (also known as nanographenes) can have very different properties – for example,...
Using a clever technique that causes unruly crystals of iron selenide to snap into alignment, Rice University physicists have drawn a detailed map that reveals...
University of Texas and MIT researchers create virtual UAVs that can predict vehicle health, enable autonomous decision-making
In the not too distant future, we can expect to see our skies filled with unmanned aerial vehicles (UAVs) delivering packages, maybe even people, from location...
03.12.2019 | Event News
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15.11.2019 | Event News
12.12.2019 | Health and Medicine
12.12.2019 | Life Sciences
12.12.2019 | Agricultural and Forestry Science