Researchers at Oxford University’s Department of Biochemistry have developed methods for making RNA duplexes and single-stranded RNAs of desired length and sequence. This exciting technology is most applicable to commercial RNA providers and companies with large in-house requirements for RNA molecules as it will greatly increase cost-effectiveness.
Small interfering ribonucleic acids (siRNAs) are powerful laboratory tools for directed post- transcriptional gene expression knockdown and inhibition of viral propagation. For siRNA to be active, it is important that the overhang in the antisense strand is complementary to the target messenger RNA. Exogenous siRNA is frequently used in RNAi studies using chemically synthesised RNA oligonucleotides to identify reagents with optimal activity.
Chemical synthesis of RNAs is relatively straightforward, but can be prohibitively expensive. Intracellular expression provides a source of continuous production of RNA in the cell, but it offers little control over the quantity of the expressed RNA and the sequence length. In vitro transcription is relatively cheap and offers a good approach to synthesis of large quantities of RNA. Unfortunately, in vitro transcription is limited by specific sequence requirements that greatly reduce the number of potential target sites for siRNA selection. Highly efficient promoters cannot be used, due to the leader sequence being transcribed and incorporated into the siRNA, leading to a further disadvantage. The inclusion of these leader sequences ultimately prevents the siRNA from efficiently functioning in RNA interference.
Jennifer Johnson | alfa
New technique for in-cell distance determination
19.03.2019 | Universität Konstanz
Dalian Coherent Light Source reveals hydroxyl super rotors from water photochemistry
19.03.2019 | Chinese Academy of Sciences Headquarters
The Potsdam Echelle Polarimetric and Spectroscopic Instrument (PEPSI) at the Large Binocular Telescope (LBT) in Arizona released its first image of the surface magnetic field of another star. In a paper in the European journal Astronomy & Astrophysics, the PEPSI team presents a Zeeman- Doppler-Image of the surface of the magnetically active star II Pegasi.
A special technique allows astronomers to resolve the surfaces of faraway stars. Those are otherwise only seen as point sources, even in the largest telescopes...
Researchers at Chalmers University of Technology and the University of Gothenburg, Sweden, have proposed a way to create a completely new source of radiation. Ultra-intense light pulses consist of the motion of a single wave and can be described as a tsunami of light. The strong wave can be used to study interactions between matter and light in a unique way. Their research is now published in the scientific journal Physical Review Letters.
"This source of radiation lets us look at reality through a new angle - it is like twisting a mirror and discovering something completely different," says...
New research group at the University of Jena combines theory and experiment to demonstrate for the first time certain physical processes in a quantum vacuum
For most people, a vacuum is an empty space. Quantum physics, on the other hand, assumes that even in this lowest-energy state, particles and antiparticles...
Physicists in the EPic Lab at University of Sussex make crucial development in global race to develop a portable atomic clock
Scientists in the Emergent Photonics Lab (EPic Lab) at the University of Sussex have made a breakthrough to a crucial element of an atomic clock - devices...
Every year earthquakes worldwide claim hundreds or even thousands of lives. Forewarning allows people to head for safety and a matter of seconds could spell...
11.03.2019 | Event News
01.03.2019 | Event News
28.02.2019 | Event News
19.03.2019 | Physics and Astronomy
19.03.2019 | Life Sciences
19.03.2019 | Physics and Astronomy