The paper, “Mapping Dynamic Protein Interactions in the MAP Kinase Signaling Using Live-Cell Fluorescence Fluctuation Spectroscopy and Imaging,” was posted to the Web site of Proceedings of the National Academy of Sciences (PNAS) yesterday and will appear in a future print issue of the journal.
In this work, Brian Slaughter, Ph.D., Postdoctoral Research Fellow; Joel Schwartz, Ph.D., Managing Director of the Imaging Center; and Rong Li, Ph.D., Investigator, used sophisticated biophysical techniques to perform quantitative biochemical measurements directly in live yeast cells.
“It turns out that by using three fluorescence-based analyses we could assess the movement, concentration, and state of protein hetero- and homo-oligomerization at the single cell level,” said Dr. Slaughter. “It is a significant advance to be able to apply these quantitative techniques to the model system of yeast.”
“These technical breakthroughs represent an exciting emerging direction for molecular analysis in the future,” said Dr. Li. “They will enable biological systems to be understood with precise information regarding when, where, and to what extent molecules interact with each other during important regulatory processes.”
The Rong Li lab worked closely with the Institute’s Imaging Center to perfect the application of these techniques in yeast, calling on the Center’s expertise and cutting-edge instrumentation for microscopy-based technology.
“This work demonstrates the Institute’s tremendous strength for live-cell quantitative analysis,” said Robb Krumlauf, Ph.D., Scientific Director. “I believe this and similar techniques will become increasingly important to our ability to better understand the most fundamental events in the life cycle of a cell.”
Marie Jennings | EurekAlert!
Study clarifies kinship of important plant group
05.08.2020 | Rheinische Friedrich-Wilhelms-Universität Bonn
Human cell-based test systems for toxicity studies: Ready-to-use Toxicity Assay (hiPSC)
05.08.2020 | Fraunhofer-Institut für Biomedizinische Technik IBMT
An international research team has found a new approach that may be able to reduce bone loss in osteoporosis and maintain bone health.
Osteoporosis is the most common age-related bone disease which affects hundreds of millions of individuals worldwide. It is estimated that one in three women...
Traditional single-cell sequencing methods help to reveal insights about cellular differences and functions - but they do this with static snapshots only...
“Core-shell” clusters pave the way for new efficient nanomaterials that make catalysts, magnetic and laser sensors or measuring devices for detecting electromagnetic radiation more efficient.
Whether in innovative high-tech materials, more powerful computer chips, pharmaceuticals or in the field of renewable energies, nanoparticles – smallest...
An international research team with Prof. Cornelia Denz from the Institute of Applied Physics at the University of Münster develop for the first time light fields using caustics that do not change during propagation. With the new method, the physicists cleverly exploit light structures that can be seen in rainbows or when light is transmitted through drinking glasses.
Modern applications as high resolution microsopy or micro- or nanoscale material processing require customized laser beams that do not change during...
Although no life has been detected on the Martian surface, a new study from astrophysicist and research scientist at the Center for Space Science at NYU Abu...
23.07.2020 | Event News
21.07.2020 | Event News
07.07.2020 | Event News
05.08.2020 | Physics and Astronomy
05.08.2020 | Health and Medicine
05.08.2020 | Earth Sciences