Forum for Science, Industry and Business

Sponsored by:     3M 
Search our Site:

 

LIFTSYS prototype machine for transferring biomaterials

29.07.2013
At the Fraunhofer Institute for Laser Technology ILT, an interdisciplinary team of researchers is working on a method for transferring biomaterials and on innovative systems technology.

Now the scientists in Aachen have managed to manufacture a machine for medical and pharmaceutical research based on the laser-induced forward transfer (LIFT) method.


Transferring biomaterials to a microarray chip using the LIFT method.
Source: Fraunhofer ILT, Aachen


LIFTSYS machine at Fraunhofer ILT, used for selectively transferring biomaterials.
Source: Fraunhofer ILT, Aachen

The system is to be used mainly for the selective transfer of hydrogels, living cells, and other biomaterials. The first prototype machine, known as LIFTSYS, was recently delivered to the Swiss Federal Institute of Technology in Lausanne EPFL.

The LIFT method can be used whenever tiny amounts of material need to be applied onto receiver substrates with pinpoint precision. One broad field of application for the technology is medical and pharmaceutical research, for instance, where diseases and active pharmaceutical ingredients are studied in specially made test structures. Here it is imperative to apply the precious material selectively and as sparingly as possible onto a receiver substrate. The LIFT method facilitates the transfer of a broad range of materials, such as glycoproteins, living cells, and metals – with high precision and using up a minimum of resources. The Biofabrication Group at Fraunhofer ILT is currently working on further developing complex cell-based in vitro test systems.

Transferring material without a printer head: cost-effective and reliable

This is how the printing process works: the receiver substrate is situated beneath a glass slide bearing the biomaterial to be transferred on its underside and an intermediate titanium absorber layer. A pulsed laser beam evaporates the titanium layer, and the resulting forwards impulse transfers the biomaterial onto the receiver substrate. This laser-based process has no need of a printer head and so it can transfer biomaterials such as RNA, DNA, proteins, and cells regardless of their viscosity. The absence of a printer head also means there is none of the associated sample wastage caused, for example, by feeder lines. This dramatically reduces the amount of material required to carry out an analysis. What is more, the LIFT method can produce spot sizes of 10 µm to 300 µm – which means up to 500,000 protein spots can fit onto a surface the size of a thumbnail. Until now it has not been possible to build up sample material with such precision and efficiency and in such small amounts.

From laboratory setups to a user-friendly machine

Fraunhofer ILT’s system development resulted in an innovative five-axis machine with motion systems for transfer and receiver substrates. The built-in beam source can be set to the wavelengths 355 nm or 1064 nm, and the focal position, laser power, and number of pulses can be automatically controlled. This enables the user to transfer a wide range of substances, from biomaterials to metals, with the LIFTSYS machine.

Researchers from the Process Control and System Technology Group in Aachen further developed the initial laboratory setups into the LIFTSYS machine. A main focus of their work was to ensure that the prototype was intuitive to operate. To this end, they integrated PC-based visualization and control technology into the system. There are two easy operating concepts for users to choose from: one is a graphical user interface, from which all elements of the system can be easily controlled; and the other is text-based programming in G-code. In addition to positioning commands, this text language also contains add-ons for laser processing: for example, laser pulses can be triggered individually and pulse energies changed. This makes it possible to program complex transfer patterns and assign them to a specific processing result.

Applications at EPFL in Lausanne

The Swiss Federal Institute of Technology in Lausanne EPFL is performing research in inkjet printing for various applications in micro-engineering, material science and bio-engineering. “The new LIFT method is a very interesting alternative to conventional nozzle-based inkjet printing, as new classes of materials can be locally deposited with low material waste,” explains Prof. Juergen Brugger of the EPFL. “The fact that even very high viscous liquids and solid films can be locally transferred to a receiver substrate makes the technique very versatile for several of our research groups.” The scientists at EPFL will first perform basic research to study the morphology and materials properties of the deposited pattern and will then benchmark the LIFT method with conventional inkjet printing. Subsequently, research for selected applications in the fields of semiconductors, biomaterials or sensors will be addressed. EPFL will also allow students to work on the new LIFT tool, preparing thus future engineers and scientists to be familiar with innovative surface patterning methods to enable novel manufacturing schemes and ultimately new applications.

LIFTSYS at Biotechnica 2013

At this year’s Biotechnica – Europe’s no.1 event for biotechnology, life sciences, and lab technology, which will take place in Hannover, Germany, from October 8 to 10, 2013 – Fraunhofer ILT will be presenting its LIFTSYS machine for the application field of in vitro test systems. At the joint Fraunhofer booth in Hall 9.E09, experts will be demonstrating a prototype machine along with other exhibits from the field of biofabrication.

Contact

Dr. Martin Wehner
Head Biotechnology and Laser Therapy Group
Telefon: +49 241 8906-202
martin.wehner@ilt.fraunhofer.de

Petra Nolis | Fraunhofer-Institut
Further information:
http://www.ilt.fraunhofer.de

More articles from Process Engineering:

nachricht New technology for mass-production of complex molded composite components
23.01.2017 | Evonik Industries AG

nachricht Etching Microstructures with Lasers
25.10.2016 | Fraunhofer-Institut für Lasertechnik ILT

All articles from Process Engineering >>>

The most recent press releases about innovation >>>

Die letzten 5 Focus-News des innovations-reports im Überblick:

Im Focus: Quantum optical sensor for the first time tested in space – with a laser system from Berlin

For the first time ever, a cloud of ultra-cold atoms has been successfully created in space on board of a sounding rocket. The MAIUS mission demonstrates that quantum optical sensors can be operated even in harsh environments like space – a prerequi-site for finding answers to the most challenging questions of fundamental physics and an important innovation driver for everyday applications.

According to Albert Einstein's Equivalence Principle, all bodies are accelerated at the same rate by the Earth's gravity, regardless of their properties. This...

Im Focus: Traffic jam in empty space

New success for Konstanz physicists in studying the quantum vacuum

An important step towards a completely new experimental access to quantum physics has been made at University of Konstanz. The team of scientists headed by...

Im Focus: How gut bacteria can make us ill

HZI researchers decipher infection mechanisms of Yersinia and immune responses of the host

Yersiniae cause severe intestinal infections. Studies using Yersinia pseudotuberculosis as a model organism aim to elucidate the infection mechanisms of these...

Im Focus: Interfacial Superconductivity: Magnetic and superconducting order revealed simultaneously

Researchers from the University of Hamburg in Germany, in collaboration with colleagues from the University of Aarhus in Denmark, have synthesized a new superconducting material by growing a few layers of an antiferromagnetic transition-metal chalcogenide on a bismuth-based topological insulator, both being non-superconducting materials.

While superconductivity and magnetism are generally believed to be mutually exclusive, surprisingly, in this new material, superconducting correlations...

Im Focus: Studying fundamental particles in materials

Laser-driving of semimetals allows creating novel quasiparticle states within condensed matter systems and switching between different states on ultrafast time scales

Studying properties of fundamental particles in condensed matter systems is a promising approach to quantum field theory. Quasiparticles offer the opportunity...

All Focus news of the innovation-report >>>

Anzeige

Anzeige

Event News

Sustainable Water use in Agriculture in Eastern Europe and Central Asia

19.01.2017 | Event News

12V, 48V, high-voltage – trends in E/E automotive architecture

10.01.2017 | Event News

2nd Conference on Non-Textual Information on 10 and 11 May 2017 in Hannover

09.01.2017 | Event News

 
Latest News

Arctic melt ponds form when meltwater clogs ice pores

24.01.2017 | Earth Sciences

Synthetic nanoparticles achieve the complexity of protein molecules

24.01.2017 | Life Sciences

PPPL physicist uncovers clues to mechanism behind magnetic reconnection

24.01.2017 | Physics and Astronomy

VideoLinks
B2B-VideoLinks
More VideoLinks >>>