Embryonic photosynthesis leads to the production of seed-internal oxygen that is important for seed development and quality. In order to visualize seed-internal structures that could serve for oxygen storage conventional microscopic methods could not be used because they require the seed to be cut thus leading to air escape. By using holotomography at the ESRF, scientists could get the full picture of an arabidopsis seed without any structural modification.
Researchers have identified individual cells within the seed and rendered them to show their three-dimensional organization. They have also distinguished an intercellular air network, which should represent an important circulation system for air and perhaps water during germination. However, scientists can’t yet assure that this is the path the oxygen follows to "feed" the seed: “Solving this question needs a nano-method to determine the exact composition of air in the network during seed formation, but unfortunately this method is not available yet”, explains Silva Lerbs-Mache, the corresponding author of the paper,.
The scientists used hard X-ray-based quantitative phase tomography at beamline ID19 to obtain three-dimensional images of an arabidopsis seed. This seed is a model plant for biologists and the first one the genome was sequenced. “This approach is to our knowledge the only imaging technique with the penetration capacity and imaged field size suited for an investigation at sub-micrometer resolution of an optically opaque object the size of a seed” explains Peter Cloetens, first author of the paper and scientist at the ESRF. It is applied for the first time to an autonomous living system, observed without object destruction, without staining, in air, and at room temperature.
The discovery of a void network opens the field of new research linking embryonic photosynthesis and the structure of the mature seed, in relation to seed quality, i. e. the capacity and vigour of germination. “The method could now be applied to study the seed structure of mutant plants that are deficient in germination and thus to link the mutation of one gene to changes in seed structure”, explains Silva Lerbs-Mache,.
Montserrat Capellas | alfa
Graphene and quantum dots put in motion a CMOS-integrated camera that can see the invisible
30.05.2017 | ICFO-The Institute of Photonic Sciences
New Method of Characterizing Graphene
30.05.2017 | Universität Basel
Scientists have developed a new method of characterizing graphene’s properties without applying disruptive electrical contacts, allowing them to investigate both the resistance and quantum capacitance of graphene and other two-dimensional materials. Researchers from the Swiss Nanoscience Institute and the University of Basel’s Department of Physics reported their findings in the journal Physical Review Applied.
Graphene consists of a single layer of carbon atoms. It is transparent, harder than diamond and stronger than steel, yet flexible, and a significantly better...
The world's highest gain high power laser amplifier - by many orders of magnitude - has been developed in research led at the University of Strathclyde.
The researchers demonstrated the feasibility of using plasma to amplify short laser pulses of picojoule-level energy up to 100 millijoules, which is a 'gain'...
Staphylococcus aureus is a feared pathogen (MRSA, multi-resistant S. aureus) due to frequent resistances against many antibiotics, especially in hospital infections. Researchers at the Paul-Ehrlich-Institut have identified immunological processes that prevent a successful immune response directed against the pathogenic agent. The delivery of bacterial proteins with RNA adjuvant or messenger RNA (mRNA) into immune cells allows the re-direction of the immune response towards an active defense against S. aureus. This could be of significant importance for the development of an effective vaccine. PLOS Pathogens has published these research results online on 25 May 2017.
Staphylococcus aureus (S. aureus) is a bacterium that colonizes by far more than half of the skin and the mucosa of adults, usually without causing infections....
Physicists from the University of Würzburg are capable of generating identical looking single light particles at the push of a button. Two new studies now demonstrate the potential this method holds.
The quantum computer has fuelled the imagination of scientists for decades: It is based on fundamentally different phenomena than a conventional computer....
An international team of physicists has monitored the scattering behaviour of electrons in a non-conducting material in real-time. Their insights could be beneficial for radiotherapy.
We can refer to electrons in non-conducting materials as ‘sluggish’. Typically, they remain fixed in a location, deep inside an atomic composite. It is hence...
24.05.2017 | Event News
23.05.2017 | Event News
22.05.2017 | Event News
30.05.2017 | Life Sciences
30.05.2017 | Life Sciences
30.05.2017 | Physics and Astronomy