Forum for Science, Industry and Business

Sponsored by:     3M 
Search our Site:

 

Researchers watch seeds in 3D and discover an unknown air path

18.09.2006
Researchers from the CNRS, the University J. Fourier (UJF) of Grenoble and the ESRF have recently visualised a plant seed in 3D using synchrotron light. This new view has revealed that there is a network of voids between the cells which may be used for oxygen storage that is needed for efficient germination. It is the first time that a living organism is studied using the holotomography technique at a third generation synchrotron source. The team behind the discovery publishes its results in PNAS this week.

Embryonic photosynthesis leads to the production of seed-internal oxygen that is important for seed development and quality. In order to visualize seed-internal structures that could serve for oxygen storage conventional microscopic methods could not be used because they require the seed to be cut thus leading to air escape. By using holotomography at the ESRF, scientists could get the full picture of an arabidopsis seed without any structural modification.

Researchers have identified individual cells within the seed and rendered them to show their three-dimensional organization. They have also distinguished an intercellular air network, which should represent an important circulation system for air and perhaps water during germination. However, scientists can’t yet assure that this is the path the oxygen follows to "feed" the seed: “Solving this question needs a nano-method to determine the exact composition of air in the network during seed formation, but unfortunately this method is not available yet”, explains Silva Lerbs-Mache, the corresponding author of the paper,.

The scientists used hard X-ray-based quantitative phase tomography at beamline ID19 to obtain three-dimensional images of an arabidopsis seed. This seed is a model plant for biologists and the first one the genome was sequenced. “This approach is to our knowledge the only imaging technique with the penetration capacity and imaged field size suited for an investigation at sub-micrometer resolution of an optically opaque object the size of a seed” explains Peter Cloetens, first author of the paper and scientist at the ESRF. It is applied for the first time to an autonomous living system, observed without object destruction, without staining, in air, and at room temperature.

The discovery of a void network opens the field of new research linking embryonic photosynthesis and the structure of the mature seed, in relation to seed quality, i. e. the capacity and vigour of germination. “The method could now be applied to study the seed structure of mutant plants that are deficient in germination and thus to link the mutation of one gene to changes in seed structure”, explains Silva Lerbs-Mache,.

Montserrat Capellas | alfa
Further information:
http://www.esrf.fr/NewsAndEvents/PressReleases/seedsin3D/

More articles from Physics and Astronomy:

nachricht Moon's crust underwent resurfacing after forming from magma ocean
22.11.2017 | University of Texas at Austin

nachricht NASA's James Webb Space Telescope completes final cryogenic testing
21.11.2017 | NASA/Goddard Space Flight Center

All articles from Physics and Astronomy >>>

The most recent press releases about innovation >>>

Die letzten 5 Focus-News des innovations-reports im Überblick:

Im Focus: Nanoparticles help with malaria diagnosis – new rapid test in development

The WHO reports an estimated 429,000 malaria deaths each year. The disease mostly affects tropical and subtropical regions and in particular the African continent. The Fraunhofer Institute for Silicate Research ISC teamed up with the Fraunhofer Institute for Molecular Biology and Applied Ecology IME and the Institute of Tropical Medicine at the University of Tübingen for a new test method to detect malaria parasites in blood. The idea of the research project “NanoFRET” is to develop a highly sensitive and reliable rapid diagnostic test so that patient treatment can begin as early as possible.

Malaria is caused by parasites transmitted by mosquito bite. The most dangerous form of malaria is malaria tropica. Left untreated, it is fatal in most cases....

Im Focus: A “cosmic snake” reveals the structure of remote galaxies

The formation of stars in distant galaxies is still largely unexplored. For the first time, astron-omers at the University of Geneva have now been able to closely observe a star system six billion light-years away. In doing so, they are confirming earlier simulations made by the University of Zurich. One special effect is made possible by the multiple reflections of images that run through the cosmos like a snake.

Today, astronomers have a pretty accurate idea of how stars were formed in the recent cosmic past. But do these laws also apply to older galaxies? For around a...

Im Focus: Visual intelligence is not the same as IQ

Just because someone is smart and well-motivated doesn't mean he or she can learn the visual skills needed to excel at tasks like matching fingerprints, interpreting medical X-rays, keeping track of aircraft on radar displays or forensic face matching.

That is the implication of a new study which shows for the first time that there is a broad range of differences in people's visual ability and that these...

Im Focus: Novel Nano-CT device creates high-resolution 3D-X-rays of tiny velvet worm legs

Computer Tomography (CT) is a standard procedure in hospitals, but so far, the technology has not been suitable for imaging extremely small objects. In PNAS, a team from the Technical University of Munich (TUM) describes a Nano-CT device that creates three-dimensional x-ray images at resolutions up to 100 nanometers. The first test application: Together with colleagues from the University of Kassel and Helmholtz-Zentrum Geesthacht the researchers analyzed the locomotory system of a velvet worm.

During a CT analysis, the object under investigation is x-rayed and a detector measures the respective amount of radiation absorbed from various angles....

Im Focus: Researchers Develop Data Bus for Quantum Computer

The quantum world is fragile; error correction codes are needed to protect the information stored in a quantum object from the deteriorating effects of noise. Quantum physicists in Innsbruck have developed a protocol to pass quantum information between differently encoded building blocks of a future quantum computer, such as processors and memories. Scientists may use this protocol in the future to build a data bus for quantum computers. The researchers have published their work in the journal Nature Communications.

Future quantum computers will be able to solve problems where conventional computers fail today. We are still far away from any large-scale implementation,...

All Focus news of the innovation-report >>>

Anzeige

Anzeige

Event News

Ecology Across Borders: International conference brings together 1,500 ecologists

15.11.2017 | Event News

Road into laboratory: Users discuss biaxial fatigue-testing for car and truck wheel

15.11.2017 | Event News

#Berlin5GWeek: The right network for Industry 4.0

30.10.2017 | Event News

 
Latest News

UCLA engineers use deep learning to reconstruct holograms and improve optical microscopy

22.11.2017 | Medical Engineering

Watching atoms move in hybrid perovskite crystals reveals clues to improving solar cells

22.11.2017 | Materials Sciences

New study points the way to therapy for rare cancer that targets the young

22.11.2017 | Health and Medicine

VideoLinks
B2B-VideoLinks
More VideoLinks >>>