Embryonic photosynthesis leads to the production of seed-internal oxygen that is important for seed development and quality. In order to visualize seed-internal structures that could serve for oxygen storage conventional microscopic methods could not be used because they require the seed to be cut thus leading to air escape. By using holotomography at the ESRF, scientists could get the full picture of an arabidopsis seed without any structural modification.
Researchers have identified individual cells within the seed and rendered them to show their three-dimensional organization. They have also distinguished an intercellular air network, which should represent an important circulation system for air and perhaps water during germination. However, scientists can’t yet assure that this is the path the oxygen follows to "feed" the seed: “Solving this question needs a nano-method to determine the exact composition of air in the network during seed formation, but unfortunately this method is not available yet”, explains Silva Lerbs-Mache, the corresponding author of the paper,.
The scientists used hard X-ray-based quantitative phase tomography at beamline ID19 to obtain three-dimensional images of an arabidopsis seed. This seed is a model plant for biologists and the first one the genome was sequenced. “This approach is to our knowledge the only imaging technique with the penetration capacity and imaged field size suited for an investigation at sub-micrometer resolution of an optically opaque object the size of a seed” explains Peter Cloetens, first author of the paper and scientist at the ESRF. It is applied for the first time to an autonomous living system, observed without object destruction, without staining, in air, and at room temperature.
The discovery of a void network opens the field of new research linking embryonic photosynthesis and the structure of the mature seed, in relation to seed quality, i. e. the capacity and vigour of germination. “The method could now be applied to study the seed structure of mutant plants that are deficient in germination and thus to link the mutation of one gene to changes in seed structure”, explains Silva Lerbs-Mache,.
Montserrat Capellas | alfa
Astronomers find unexpected, dust-obscured star formation in distant galaxy
24.03.2017 | University of Massachusetts at Amherst
Gravitational wave kicks monster black hole out of galactic core
24.03.2017 | NASA/Goddard Space Flight Center
Astronomers from Bonn and Tautenburg in Thuringia (Germany) used the 100-m radio telescope at Effelsberg to observe several galaxy clusters. At the edges of these large accumulations of dark matter, stellar systems (galaxies), hot gas, and charged particles, they found magnetic fields that are exceptionally ordered over distances of many million light years. This makes them the most extended magnetic fields in the universe known so far.
The results will be published on March 22 in the journal „Astronomy & Astrophysics“.
Galaxy clusters are the largest gravitationally bound structures in the universe. With a typical extent of about 10 million light years, i.e. 100 times the...
Researchers at the Goethe University Frankfurt, together with partners from the University of Tübingen in Germany and Queen Mary University as well as Francis Crick Institute from London (UK) have developed a novel technology to decipher the secret ubiquitin code.
Ubiquitin is a small protein that can be linked to other cellular proteins, thereby controlling and modulating their functions. The attachment occurs in many...
In the eternal search for next generation high-efficiency solar cells and LEDs, scientists at Los Alamos National Laboratory and their partners are creating...
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