The findings, appear in the June issue of the Proceedings of the National Academy of Sciences, and have important implications for creating better therapeutics against diarrheal diseases.
According to the World Health Organization, enterotoxigenic Escherichia coli (ETEC) bacteria causes the largest number of recorded community-acquired cases of childhood diarrhea in the developing world, and is the most common culprit in traveler's diarrhea.
ETEC uses surface "pili" or "fimbriae" to attach to host intestinal epithelia, an early, vital step in diarrhea pathogenesis. These fibers are essential for initiation of infection in the intestines by ETEC. According to the researchers, this study elucidates the mechanics of assembly of the fimbriae helical fibers as they exit the bacterium through a pore on the bacterial surface.
"Atomic resolution detail of the proteins in the fibers and analysis of genetic variability among different clinical strains were combined to show that each bacterial strain presents a different outer surface of the major protein while preserving the protein components that are buried within the fiber," said senior author Esther Bullitt, an associate professor in the department of physiology and biophysics at BUSM. "Because of this, the antibodies produced against ETEC during one episode of infection are often not protective against later infections by other strains," she added.
As a result of this new study, the researchers believe that developing a cross-protective vaccine will require a strategy that focuses on use of the tip protein as an antigen. "The structural findings reported here have bearing on research to help guide vaccine development."
Funding for this study was provided by the NIH (NIGMS) and NCI) the United States Army and the Henry M. Jackson Foundation.
Gina DiGravio | EurekAlert!
23.03.2017 | Technische Universität München
How prenatal maternal infections may affect genetic factors in Autism spectrum disorder
22.03.2017 | University of California - San Diego
Astronomers from Bonn and Tautenburg in Thuringia (Germany) used the 100-m radio telescope at Effelsberg to observe several galaxy clusters. At the edges of these large accumulations of dark matter, stellar systems (galaxies), hot gas, and charged particles, they found magnetic fields that are exceptionally ordered over distances of many million light years. This makes them the most extended magnetic fields in the universe known so far.
The results will be published on March 22 in the journal „Astronomy & Astrophysics“.
Galaxy clusters are the largest gravitationally bound structures in the universe. With a typical extent of about 10 million light years, i.e. 100 times the...
Researchers at the Goethe University Frankfurt, together with partners from the University of Tübingen in Germany and Queen Mary University as well as Francis Crick Institute from London (UK) have developed a novel technology to decipher the secret ubiquitin code.
Ubiquitin is a small protein that can be linked to other cellular proteins, thereby controlling and modulating their functions. The attachment occurs in many...
In the eternal search for next generation high-efficiency solar cells and LEDs, scientists at Los Alamos National Laboratory and their partners are creating...
Silicon nanosheets are thin, two-dimensional layers with exceptional optoelectronic properties very similar to those of graphene. Albeit, the nanosheets are less stable. Now researchers at the Technical University of Munich (TUM) have, for the first time ever, produced a composite material combining silicon nanosheets and a polymer that is both UV-resistant and easy to process. This brings the scientists a significant step closer to industrial applications like flexible displays and photosensors.
Silicon nanosheets are thin, two-dimensional layers with exceptional optoelectronic properties very similar to those of graphene. Albeit, the nanosheets are...
Enzymes behave differently in a test tube compared with the molecular scrum of a living cell. Chemists from the University of Basel have now been able to simulate these confined natural conditions in artificial vesicles for the first time. As reported in the academic journal Small, the results are offering better insight into the development of nanoreactors and artificial organelles.
Enzymes behave differently in a test tube compared with the molecular scrum of a living cell. Chemists from the University of Basel have now been able to...
20.03.2017 | Event News
14.03.2017 | Event News
07.03.2017 | Event News
23.03.2017 | Life Sciences
23.03.2017 | Power and Electrical Engineering
23.03.2017 | Earth Sciences