Cardiac injury leads to significant structural changes in the heart, including enlargement, excess formation of fibrous growth tissue, and abnormalities of the coronary vasculature. While associated factors have been targeted for therapeutic intervention, the results have been conflicting.
Most studies have investigated these changes after six days of injury. However, advanced stages of remodeling have already begun by day seven following injury. New research reveals that morphological changes in response to cardiac injury occur rapidly, with implications for the development of therapeutic strategies. The results are published in the October issue of The American Journal of Pathology.
"We analyzed and have demonstrated for the first time the early interaction and coordination of morphological changes, cell populations, and gene expression following pathological cardiac insult," reports lead investigator Troy A. Baudino, PhD, of the Cardiovascular Research Institute at Texas A&M Health Science Center, Temple, TX. "The time course of our study allows these events to be correlated to one another, providing valuable insight for future studies of cardiac pathology."
The investigators induced heart injury in mice through transverse aortic constriction (TAC). A control group of mice underwent a surgical procedure without the aortic constriction, for comparison. They evaluated acute cardiac modeling events beginning two days after surgery, including changes in hypertrophy, collagen deposition, capillary density, and cell populations.
Within 48 hours after injury, the left ventricular free wall and septum were significantly enlarged, with an increase in heart weight and relative wall thickness compared to controls. In addition to this hypertrophy, a significant decrease in capillary density was observed two days after TAC. Increased levels of pericytes, which are connective tissue cells in small blood vessels, were associated with the reduction in capillary density, supporting earlier research that suggested a role for pericytes in stabilizing vessels and minimizing vascular remodeling. "The participation of pericytes could mark the period where degradation transitions to new capillary formation and re-vascularization," says Dr. Baudino.
Investigators observed increased fibroblasts and collagen seven days following TAC, contributing to the increased heart weight between two and seven days after injury. "We found that these changes take place concurrently with vascular re-growth," says Dr. Baudino. "It is possible that while the expanding fibroblast population is depositing excess extracellular material, a process that stiffens the heart, it also simultaneously stimulates capillary growth, to deliver more oxygen and nutrients to the expanding myocardium. Given this implication, regulation of fibroblasts may offer an advantageous route of therapy."
Dr. Baudino notes that the human response to pressure overload such as hypertension is more gradual than in the TAC mouse model and may vary with respect to the specific timing of remodeling. "Future studies should examine the remodeling events in a clinical setting. However, our results provide a basis for investigating how these events are coordinated during remodeling and the importance of possible intervention before pathological remodeling appears," he concludes.
David Sampson | EurekAlert!
NIH scientists describe potential antibody treatment for multidrug-resistant K. pneumoniae
14.03.2018 | NIH/National Institute of Allergy and Infectious Diseases
Researchers identify key step in viral replication
13.03.2018 | University of Pittsburgh Schools of the Health Sciences
Animal photoreceptors capture light with photopigments. Researchers from the University of Göttingen have now discovered that these photopigments fulfill an...
On 15 March, the AWI research aeroplane Polar 5 will depart for Greenland. Concentrating on the furthest northeast region of the island, an international team...
The world’s second-largest ice shelf was the destination for a Polarstern expedition that ended in Punta Arenas, Chile on 14th March 2018. Oceanographers from...
At the 2018 ILA Berlin Air Show from April 25–29, the Fraunhofer Institute for Laser Technology ILT is showcasing extreme high-speed Laser Material Deposition (EHLA): A video documents how for metal components that are highly loaded, EHLA has already proved itself as an alternative to hard chrome plating, which is now allowed only under special conditions.
When the EU restricted the use of hexavalent chromium compounds to special applications requiring authorization, the move prompted a rethink in the surface...
At the ILA Berlin, hall 4, booth 202, Fraunhofer FHR will present two radar sensors for navigation support of drones. The sensors are valuable components in the implementation of autonomous flying drones: they function as obstacle detectors to prevent collisions. Radar sensors also operate reliably in restricted visibility, e.g. in foggy or dusty conditions. Due to their ability to measure distances with high precision, the radar sensors can also be used as altimeters when other sources of information such as barometers or GPS are not available or cannot operate optimally.
Drones play an increasingly important role in the area of logistics and services. Well-known logistic companies place great hope in these compact, aerial...
16.03.2018 | Event News
13.03.2018 | Event News
08.03.2018 | Event News
16.03.2018 | Earth Sciences
16.03.2018 | Physics and Astronomy
16.03.2018 | Life Sciences