In an article in the current issue of Spectroscopy, researchers at the Ohio State University College of Medicine and Public Health describe the clinical evaluation of thrombotic thrombocytopenic purpura (TTP) using Surface Enhanced Laser Desorption/Ionization Time of Flight (SELDI-TOF) mass spectrometry.
TTP is a condition caused by a deficiency in a metalloproteinase called ADAMTS13. With a timely diagnosis, TTP patients usually respond to plasma exchange therapy. However, many patients relapse after therapy. Thus, there is a need for both a rapid diagnostic test to help clinicians start therapy and for a biomarker that can anticipate recurrence.
Without enough ADAMTS13, extensive blood clots may occur throughout the body and can cause death. ADAMTS13 prevents this by breaking apart another protein that causes the clotting process. By measuring one of the broken fragments using SELDI-TOF, the researchers found that they could determine ADAMTS13 activity levels in the patient’s blood.
SELDI-TOF mass spectrometry offers an unique property to specifically capture and concentrate the target protein in a binding array. Afterwards it becomes relatively simple to measure the analyte. In this application, an immobilized metal affinity capture (IMAC) array was chosen which could bind to a product of ADAMTS13 proteolysis, ahistidine-containing fragment. The product was then measured by the mass spectrometer. Thus, the method was used to evaluate ADAMTS13 activity in TTP patients.
Writing in the article, Haifeng M. Wu, MD, states “Since the implementation of our SELDI-TOF based method, fast turnaround time for the detection of ADAMTS13 activity has greatly helped us in making the correct diagnosis, instituting appropriate therapy, and improving patient outcomes.”
The article is “Application of SELDI-TOF mass spectrometry in clinical evaluation of thrombotic thrombocytopenic purpura” by Haifeng M. Wu, Spero R. Cataland, Michael Bissell and Ming Jin. It appears in Spectroscopy, Volume 20, Issue 5/6 (2007), published by IOS Press.
Astrid Engelen | alfa
Improving memory with magnets
28.03.2017 | McGill University
Graphene-based neural probes probe brain activity in high resolution
28.03.2017 | Graphene Flagship
The Institute of Semiconductor Technology and the Institute of Physical and Theoretical Chemistry, both members of the Laboratory for Emerging Nanometrology (LENA), at Technische Universität Braunschweig are partners in a new European research project entitled ChipScope, which aims to develop a completely new and extremely small optical microscope capable of observing the interior of living cells in real time. A consortium of 7 partners from 5 countries will tackle this issue with very ambitious objectives during a four-year research program.
To demonstrate the usefulness of this new scientific tool, at the end of the project the developed chip-sized microscope will be used to observe in real-time...
Astronomers from Bonn and Tautenburg in Thuringia (Germany) used the 100-m radio telescope at Effelsberg to observe several galaxy clusters. At the edges of these large accumulations of dark matter, stellar systems (galaxies), hot gas, and charged particles, they found magnetic fields that are exceptionally ordered over distances of many million light years. This makes them the most extended magnetic fields in the universe known so far.
The results will be published on March 22 in the journal „Astronomy & Astrophysics“.
Galaxy clusters are the largest gravitationally bound structures in the universe. With a typical extent of about 10 million light years, i.e. 100 times the...
Researchers at the Goethe University Frankfurt, together with partners from the University of Tübingen in Germany and Queen Mary University as well as Francis Crick Institute from London (UK) have developed a novel technology to decipher the secret ubiquitin code.
Ubiquitin is a small protein that can be linked to other cellular proteins, thereby controlling and modulating their functions. The attachment occurs in many...
In the eternal search for next generation high-efficiency solar cells and LEDs, scientists at Los Alamos National Laboratory and their partners are creating...
Silicon nanosheets are thin, two-dimensional layers with exceptional optoelectronic properties very similar to those of graphene. Albeit, the nanosheets are less stable. Now researchers at the Technical University of Munich (TUM) have, for the first time ever, produced a composite material combining silicon nanosheets and a polymer that is both UV-resistant and easy to process. This brings the scientists a significant step closer to industrial applications like flexible displays and photosensors.
Silicon nanosheets are thin, two-dimensional layers with exceptional optoelectronic properties very similar to those of graphene. Albeit, the nanosheets are...
20.03.2017 | Event News
14.03.2017 | Event News
07.03.2017 | Event News
28.03.2017 | Life Sciences
28.03.2017 | Information Technology
28.03.2017 | Physics and Astronomy